Survivin Inhibition by Piperine Sensitizes Glioblastoma Cancer Stem Cells and Leads to Better Drug Response
(2022) In International Journal of Molecular Sciences 23(14).- Abstract
Glioblastoma multiforme (GBM) cancer stem cells (GSCs) are one of the strongest contributing factors to treatment resistance in GBM. Identification of biomarkers capable of directly affecting these cells within the bulk tumor is a major challenge associated with the development of new targeting strategies. In this study, we focus on understanding the potential of the multifunctional extraordinaire survivin as a biomarker for GSCs. We analyzed the expression profiles of this gene using various publicly available datasets to understand its importance in stemness and other cancer processes. The findings from these studies were further validated using human GSCs isolated from a GBM cell line. In these GSCs, survivin was inhibited using the... (More)
Glioblastoma multiforme (GBM) cancer stem cells (GSCs) are one of the strongest contributing factors to treatment resistance in GBM. Identification of biomarkers capable of directly affecting these cells within the bulk tumor is a major challenge associated with the development of new targeting strategies. In this study, we focus on understanding the potential of the multifunctional extraordinaire survivin as a biomarker for GSCs. We analyzed the expression profiles of this gene using various publicly available datasets to understand its importance in stemness and other cancer processes. The findings from these studies were further validated using human GSCs isolated from a GBM cell line. In these GSCs, survivin was inhibited using the dietary phytochemical piperine (PIP) and the subsequent effects on stemness, cancer processes and Temozolomide were investigated. In silico analysis identified survivin to be one of the most significant differentially regulated gene in GSCs, in comparison to common stemness markers. Further validation studies on the isolated GSCs showed the importance of survivin in stemness, cancer progression and therapy resistance. Taken together, our study identifies survivin as a more consistent GSC marker and also suggests the possibility of using survivin inhibitors along with standard of care drugs for better therapeutic outcomes.
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- author
- Warrier, Neerada Meenakshi
; Krishnan, Ramesh Kumar
; Prabhu, Vijendra
; Hariharapura, Raghu Chandrashekhar
; Agarwal, Prasoon
LU
and Kumar, Praveen
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glioblastoma cancer stem cells, integrative analysis, piperine, survivin, temozolomide, therapeutic targeting
- in
- International Journal of Molecular Sciences
- volume
- 23
- issue
- 14
- article number
- 7604
- publisher
- MDPI AG
- external identifiers
-
- pmid:35886952
- scopus:85135100984
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms23147604
- language
- English
- LU publication?
- yes
- id
- 7bedf48d-38c0-4b19-91cf-7c1b3efafb24
- date added to LUP
- 2022-10-06 10:22:25
- date last changed
- 2025-02-08 03:18:46
@article{7bedf48d-38c0-4b19-91cf-7c1b3efafb24, abstract = {{<p>Glioblastoma multiforme (GBM) cancer stem cells (GSCs) are one of the strongest contributing factors to treatment resistance in GBM. Identification of biomarkers capable of directly affecting these cells within the bulk tumor is a major challenge associated with the development of new targeting strategies. In this study, we focus on understanding the potential of the multifunctional extraordinaire survivin as a biomarker for GSCs. We analyzed the expression profiles of this gene using various publicly available datasets to understand its importance in stemness and other cancer processes. The findings from these studies were further validated using human GSCs isolated from a GBM cell line. In these GSCs, survivin was inhibited using the dietary phytochemical piperine (PIP) and the subsequent effects on stemness, cancer processes and Temozolomide were investigated. In silico analysis identified survivin to be one of the most significant differentially regulated gene in GSCs, in comparison to common stemness markers. Further validation studies on the isolated GSCs showed the importance of survivin in stemness, cancer progression and therapy resistance. Taken together, our study identifies survivin as a more consistent GSC marker and also suggests the possibility of using survivin inhibitors along with standard of care drugs for better therapeutic outcomes.</p>}}, author = {{Warrier, Neerada Meenakshi and Krishnan, Ramesh Kumar and Prabhu, Vijendra and Hariharapura, Raghu Chandrashekhar and Agarwal, Prasoon and Kumar, Praveen}}, issn = {{1661-6596}}, keywords = {{glioblastoma cancer stem cells; integrative analysis; piperine; survivin; temozolomide; therapeutic targeting}}, language = {{eng}}, number = {{14}}, publisher = {{MDPI AG}}, series = {{International Journal of Molecular Sciences}}, title = {{Survivin Inhibition by Piperine Sensitizes Glioblastoma Cancer Stem Cells and Leads to Better Drug Response}}, url = {{http://dx.doi.org/10.3390/ijms23147604}}, doi = {{10.3390/ijms23147604}}, volume = {{23}}, year = {{2022}}, }