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Cytosolic Trapping of a Mitochondrial Heat Shock Protein Is an Early Pathological Event in Synucleinopathies

Szegő, Éva M. ; Dominguez-Meijide, Antonio ; Gerhardt, Ellen ; König, Annekatrin ; Koss, David J. ; Li, Wen LU ; Pinho, Raquel ; Fahlbusch, Christiane ; Johnson, Mary and Santos, Patricia , et al. (2019) In Cell Reports 28(1). p.6-77
Abstract

Alpha-synuclein (aSyn) accumulates in intracellular inclusions in synucleinopathies, but the molecular mechanisms leading to disease are unclear. We identify the 10 kDa heat shock protein (HSP10) as a mediator of aSyn-induced mitochondrial impairments in striatal synaptosomes. We find an age-associated increase in the cytosolic levels of HSP10, and a concomitant decrease in the mitochondrial levels, in aSyn transgenic mice. The levels of superoxide dismutase 2, a client of the HSP10/HSP60 folding complex, and synaptosomal spare respiratory capacity are also reduced. Overexpression of HSP10 ameliorates aSyn-associated mitochondrial dysfunction and delays aSyn pathology in vitro and in vivo. Altogether, our data indicate that increased... (More)

Alpha-synuclein (aSyn) accumulates in intracellular inclusions in synucleinopathies, but the molecular mechanisms leading to disease are unclear. We identify the 10 kDa heat shock protein (HSP10) as a mediator of aSyn-induced mitochondrial impairments in striatal synaptosomes. We find an age-associated increase in the cytosolic levels of HSP10, and a concomitant decrease in the mitochondrial levels, in aSyn transgenic mice. The levels of superoxide dismutase 2, a client of the HSP10/HSP60 folding complex, and synaptosomal spare respiratory capacity are also reduced. Overexpression of HSP10 ameliorates aSyn-associated mitochondrial dysfunction and delays aSyn pathology in vitro and in vivo. Altogether, our data indicate that increased levels of aSyn induce mitochondrial deficits, at least partially, by sequestering HSP10 in the cytosol and preventing it from acting in mitochondria. Importantly, these alterations manifest first at presynaptic terminals. Our study not only provides mechanistic insight into synucleinopathies but opens new avenues for targeting underlying cellular pathologies. Szegő et al. identify HSP10 as a modulator of alpha-synuclein-induced mitochondrial impairment in striatal synaptosomes. Age-associated increase in the cytosolic and decrease in mitochondrial levels of HSP10 results in a reduction in the levels of SOD2 and of synaptosomal ATP production on demand. HSP10 overexpression delays alpha-synuclein pathology both in vitro and in vivo.

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Contribution to journal
publication status
published
subject
keywords
alpha-synuclein, HSP10, mitochondria, Parkinson's disease, proteostasis, striatum, synaptosome
in
Cell Reports
volume
28
issue
1
pages
6 - 77
publisher
Cell Press
external identifiers
  • scopus:85067867617
ISSN
2211-1247
DOI
10.1016/j.celrep.2019.06.009
language
English
LU publication?
yes
id
7c099183-7f3b-47b6-ab42-ea22939487bd
date added to LUP
2019-07-08 12:11:07
date last changed
2019-11-25 09:32:53
@article{7c099183-7f3b-47b6-ab42-ea22939487bd,
  abstract     = {<p>Alpha-synuclein (aSyn) accumulates in intracellular inclusions in synucleinopathies, but the molecular mechanisms leading to disease are unclear. We identify the 10 kDa heat shock protein (HSP10) as a mediator of aSyn-induced mitochondrial impairments in striatal synaptosomes. We find an age-associated increase in the cytosolic levels of HSP10, and a concomitant decrease in the mitochondrial levels, in aSyn transgenic mice. The levels of superoxide dismutase 2, a client of the HSP10/HSP60 folding complex, and synaptosomal spare respiratory capacity are also reduced. Overexpression of HSP10 ameliorates aSyn-associated mitochondrial dysfunction and delays aSyn pathology in vitro and in vivo. Altogether, our data indicate that increased levels of aSyn induce mitochondrial deficits, at least partially, by sequestering HSP10 in the cytosol and preventing it from acting in mitochondria. Importantly, these alterations manifest first at presynaptic terminals. Our study not only provides mechanistic insight into synucleinopathies but opens new avenues for targeting underlying cellular pathologies. Szegő et al. identify HSP10 as a modulator of alpha-synuclein-induced mitochondrial impairment in striatal synaptosomes. Age-associated increase in the cytosolic and decrease in mitochondrial levels of HSP10 results in a reduction in the levels of SOD2 and of synaptosomal ATP production on demand. HSP10 overexpression delays alpha-synuclein pathology both in vitro and in vivo.</p>},
  author       = {Szegő, Éva M. and Dominguez-Meijide, Antonio and Gerhardt, Ellen and König, Annekatrin and Koss, David J. and Li, Wen and Pinho, Raquel and Fahlbusch, Christiane and Johnson, Mary and Santos, Patricia and Villar-Piqué, Anna and Thom, Tobias and Rizzoli, Silvio and Schmitz, Matthias and Li, Jiayi and Zerr, Inga and Attems, Johannes and Jahn, Olaf and Outeiro, Tiago F.},
  issn         = {2211-1247},
  language     = {eng},
  number       = {1},
  pages        = {6--77},
  publisher    = {Cell Press},
  series       = {Cell Reports},
  title        = {Cytosolic Trapping of a Mitochondrial Heat Shock Protein Is an Early Pathological Event in Synucleinopathies},
  url          = {http://dx.doi.org/10.1016/j.celrep.2019.06.009},
  doi          = {10.1016/j.celrep.2019.06.009},
  volume       = {28},
  year         = {2019},
}