Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae
(1999) In FEBS Letters 462(1-2). p.37-42- Abstract
Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and... (More)
Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.
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- author
- Petersson, Jens LU ; Pattison, Johanna ; Kruckeberg, Arthur L ; Berden, Jan A and Persson, B L
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- keywords
- Carrier Proteins/biosynthesis, Cell Membrane/metabolism, Fungal Proteins/biosynthesis, Green Fluorescent Proteins, Luminescent Proteins/genetics, Phosphates/physiology, Proton-Phosphate Symporters, Recombinant Fusion Proteins/biosynthesis, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae Proteins
- in
- FEBS Letters
- volume
- 462
- issue
- 1-2
- pages
- 37 - 42
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:0032738114
- pmid:10580087
- ISSN
- 0014-5793
- DOI
- 10.1016/s0014-5793(99)01471-4
- language
- English
- LU publication?
- no
- id
- 7c242e41-7a13-4bf7-b5d3-a32a00ba253e
- date added to LUP
- 2021-07-08 16:21:26
- date last changed
- 2024-01-05 12:59:14
@article{7c242e41-7a13-4bf7-b5d3-a32a00ba253e, abstract = {{<p>Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.</p>}}, author = {{Petersson, Jens and Pattison, Johanna and Kruckeberg, Arthur L and Berden, Jan A and Persson, B L}}, issn = {{0014-5793}}, keywords = {{Carrier Proteins/biosynthesis; Cell Membrane/metabolism; Fungal Proteins/biosynthesis; Green Fluorescent Proteins; Luminescent Proteins/genetics; Phosphates/physiology; Proton-Phosphate Symporters; Recombinant Fusion Proteins/biosynthesis; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins}}, language = {{eng}}, number = {{1-2}}, pages = {{37--42}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae}}, url = {{http://dx.doi.org/10.1016/s0014-5793(99)01471-4}}, doi = {{10.1016/s0014-5793(99)01471-4}}, volume = {{462}}, year = {{1999}}, }