Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae

Petersson, Jens LU ; Pattison, Johanna ; Kruckeberg, Arthur L ; Berden, Jan A and Persson, B L (1999) In FEBS Letters 462(1-2). p.37-42
Abstract

Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and... (More)

Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Carrier Proteins/biosynthesis, Cell Membrane/metabolism, Fungal Proteins/biosynthesis, Green Fluorescent Proteins, Luminescent Proteins/genetics, Phosphates/physiology, Proton-Phosphate Symporters, Recombinant Fusion Proteins/biosynthesis, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae Proteins
in
FEBS Letters
volume
462
issue
1-2
pages
37 - 42
publisher
Wiley-Blackwell
external identifiers
  • scopus:0032738114
  • pmid:10580087
ISSN
0014-5793
DOI
10.1016/s0014-5793(99)01471-4
language
English
LU publication?
no
id
7c242e41-7a13-4bf7-b5d3-a32a00ba253e
date added to LUP
2021-07-08 16:21:26
date last changed
2024-01-05 12:59:14
@article{7c242e41-7a13-4bf7-b5d3-a32a00ba253e,
  abstract     = {{<p>Green fluorescent protein (GFP) from Aequorea victoria was used as an in vivo reporter protein when fused to the carboxy-terminus of the Pho84 phosphate permease of Saccharomyces cerevisiae. Both components of the fusion protein displayed their native functions and revealed a cellular localization and degradation of the Pho84-GFP chimera consistent with the behavior of the wild-type Pho84 protein. The GFP-tagged chimera allowed for a detection of conditions under which the Pho84 transporter is localized to its functional environment, i.e. the plasma membrane, and conditions linked to relocation of the protein to the vacuole for degradation. By use of the methodology described, GFP should be useful in studies of localization and degradation also of other membrane proteins in vivo.</p>}},
  author       = {{Petersson, Jens and Pattison, Johanna and Kruckeberg, Arthur L and Berden, Jan A and Persson, B L}},
  issn         = {{0014-5793}},
  keywords     = {{Carrier Proteins/biosynthesis; Cell Membrane/metabolism; Fungal Proteins/biosynthesis; Green Fluorescent Proteins; Luminescent Proteins/genetics; Phosphates/physiology; Proton-Phosphate Symporters; Recombinant Fusion Proteins/biosynthesis; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{37--42}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Intracellular localization of an active green fluorescent protein-tagged Pho84 phosphate permease in Saccharomyces cerevisiae}},
  url          = {{http://dx.doi.org/10.1016/s0014-5793(99)01471-4}},
  doi          = {{10.1016/s0014-5793(99)01471-4}},
  volume       = {{462}},
  year         = {{1999}},
}