Oral Microbiota Profile in Patients with Anti-Neutrophil Cytoplasmic Antibody–Associated Vasculitis
(2022) In Microorganisms 10(8).- Abstract
Microbiota has been associated with autoimmune diseases, with nasal Staphylococcus aureus being implicated in the pathogenesis of anti-neutrophil cytoplasmic antibody–associated vasculitis (AAV). Little is known about the role of oral microbiota in AAV. In this study, levels of IgG antibodies to 53 oral bacterial species/subspecies were screened using immunoblotting in plasma/serum in pre-symptomatic AAV-individuals (n = 85), matched controls, and established AAV-patients (n = 78). Saliva microbiota from acute-AAV and controls was sequenced from 16s rDNA amplicons. Information on dental status was extracted from a national register. IgG levels against oral bacteria were lower in established AAV versus pre-AAV and controls. Specifically,... (More)
Microbiota has been associated with autoimmune diseases, with nasal Staphylococcus aureus being implicated in the pathogenesis of anti-neutrophil cytoplasmic antibody–associated vasculitis (AAV). Little is known about the role of oral microbiota in AAV. In this study, levels of IgG antibodies to 53 oral bacterial species/subspecies were screened using immunoblotting in plasma/serum in pre-symptomatic AAV-individuals (n = 85), matched controls, and established AAV-patients (n = 78). Saliva microbiota from acute-AAV and controls was sequenced from 16s rDNA amplicons. Information on dental status was extracted from a national register. IgG levels against oral bacteria were lower in established AAV versus pre-AAV and controls. Specifically, pre-AAV samples had, compared to controls, a higher abundance of periodontitis-associated species paralleling more signs of periodontitis in established AAV-patients than controls. Saliva microbiota in acute-AAV showed higher within-sample diversity but fewer detectable amplicon-sequence variants and taxa in their core microbiota than controls. Acute-AAV was not associated with increased abundance of periodontal bacteria but species in, e.g., Arthrospira, Staphylococcus, Lactobacillus, and Scardovia. In conclusion, the IgG profiles against oral bacteria differed between pre-AAV, established AAV, and controls, and microbiota profiles between acute AAV and controls. The IgG shift from a pre-symptomatic stage to established disease cooccurred with treatment of immunosuppression and/or antibiotics.
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- author
- Esberg, Anders ; Johansson, Linda ; Berglin, Ewa ; Mohammad, Aladdin J. LU ; Jonsson, Andreas P. ; Dahlqvist, Johanna ; Stegmayr, Bernd ; Johansson, Ingegerd and Rantapää-Dahlqvist, Solbritt
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- anti-neutrophil cytoplasmatic antibody–associated vasculitis, caries, granulomatosis with polyangiitis (GPA), IgG, microscopic polyangiitis (MPA), myeloperoxidase (MPO)-ANCA, oral microbiota, periodontal disease, proteinase 3 (PR3)-ANCA, vasculitis
- in
- Microorganisms
- volume
- 10
- issue
- 8
- article number
- 1572
- publisher
- MDPI AG
- external identifiers
-
- scopus:85137345895
- pmid:36013990
- ISSN
- 2076-2607
- DOI
- 10.3390/microorganisms10081572
- language
- English
- LU publication?
- yes
- id
- 7c8071b5-b613-485d-a5e8-184c0974c548
- date added to LUP
- 2022-11-29 15:06:17
- date last changed
- 2024-04-14 17:24:30
@article{7c8071b5-b613-485d-a5e8-184c0974c548,
abstract = {{<p>Microbiota has been associated with autoimmune diseases, with nasal Staphylococcus aureus being implicated in the pathogenesis of anti-neutrophil cytoplasmic antibody–associated vasculitis (AAV). Little is known about the role of oral microbiota in AAV. In this study, levels of IgG antibodies to 53 oral bacterial species/subspecies were screened using immunoblotting in plasma/serum in pre-symptomatic AAV-individuals (n = 85), matched controls, and established AAV-patients (n = 78). Saliva microbiota from acute-AAV and controls was sequenced from 16s rDNA amplicons. Information on dental status was extracted from a national register. IgG levels against oral bacteria were lower in established AAV versus pre-AAV and controls. Specifically, pre-AAV samples had, compared to controls, a higher abundance of periodontitis-associated species paralleling more signs of periodontitis in established AAV-patients than controls. Saliva microbiota in acute-AAV showed higher within-sample diversity but fewer detectable amplicon-sequence variants and taxa in their core microbiota than controls. Acute-AAV was not associated with increased abundance of periodontal bacteria but species in, e.g., Arthrospira, Staphylococcus, Lactobacillus, and Scardovia. In conclusion, the IgG profiles against oral bacteria differed between pre-AAV, established AAV, and controls, and microbiota profiles between acute AAV and controls. The IgG shift from a pre-symptomatic stage to established disease cooccurred with treatment of immunosuppression and/or antibiotics.</p>}},
author = {{Esberg, Anders and Johansson, Linda and Berglin, Ewa and Mohammad, Aladdin J. and Jonsson, Andreas P. and Dahlqvist, Johanna and Stegmayr, Bernd and Johansson, Ingegerd and Rantapää-Dahlqvist, Solbritt}},
issn = {{2076-2607}},
keywords = {{anti-neutrophil cytoplasmatic antibody–associated vasculitis; caries; granulomatosis with polyangiitis (GPA); IgG; microscopic polyangiitis (MPA); myeloperoxidase (MPO)-ANCA; oral microbiota; periodontal disease; proteinase 3 (PR3)-ANCA; vasculitis}},
language = {{eng}},
number = {{8}},
publisher = {{MDPI AG}},
series = {{Microorganisms}},
title = {{Oral Microbiota Profile in Patients with Anti-Neutrophil Cytoplasmic Antibody–Associated Vasculitis}},
url = {{http://dx.doi.org/10.3390/microorganisms10081572}},
doi = {{10.3390/microorganisms10081572}},
volume = {{10}},
year = {{2022}},
}