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Practical Considerations for the Use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain

Aldrin-Kirk, Patrick LU and Björklund, Tomas LU (2019) In Methods in molecular biology (Clifton, N.J.) 1937. p.59-87
Abstract

Chemogenetics is the process of genetically expressing a macromolecule receptor capable of modulating the activity of the cell in response to selective chemical ligand. This chapter will cover the chemogenetic technologies that are available to date, focusing on the commonly available engineered or otherwise modified ligand-gated ion channels and G-protein-coupled receptors in the context of neuromodulation. First, we will give a brief overview of each chemogenetic approach as well as in vitro/in vivo applications, then we will list their strengths and weaknesses. Finally, we will provide tips for ligand application in each case.Each technology has specific limitations that make them more or less suitable for different applications in... (More)

Chemogenetics is the process of genetically expressing a macromolecule receptor capable of modulating the activity of the cell in response to selective chemical ligand. This chapter will cover the chemogenetic technologies that are available to date, focusing on the commonly available engineered or otherwise modified ligand-gated ion channels and G-protein-coupled receptors in the context of neuromodulation. First, we will give a brief overview of each chemogenetic approach as well as in vitro/in vivo applications, then we will list their strengths and weaknesses. Finally, we will provide tips for ligand application in each case.Each technology has specific limitations that make them more or less suitable for different applications in neuroscience although we will focus mainly on the most commonly used and versatile family named designer receptors exclusively activated by designer drugs or DREADDs. We here describe the most common cases where these can be implemented and provide tips on how and where these technologies can be applied in the field of neuroscience.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Allatostatin, Chemogenetics, DREADD, Engineered receptors, G-Protein-coupled receptor, Gene therapy, GluCl, Ligand, Ligand-gated ion channel, Neuronal modulation, PSAM/PSEM, TRPV1
in
Methods in molecular biology (Clifton, N.J.)
volume
1937
pages
29 pages
publisher
Springer
external identifiers
  • scopus:85060945000
ISSN
1940-6029
DOI
10.1007/978-1-4939-9065-8_4
language
English
LU publication?
yes
id
7c8d75aa-0660-4840-a423-2c51a1ea88b3
date added to LUP
2019-02-12 10:40:48
date last changed
2019-03-05 04:32:32
@article{7c8d75aa-0660-4840-a423-2c51a1ea88b3,
  abstract     = {<p>Chemogenetics is the process of genetically expressing a macromolecule receptor capable of modulating the activity of the cell in response to selective chemical ligand. This chapter will cover the chemogenetic technologies that are available to date, focusing on the commonly available engineered or otherwise modified ligand-gated ion channels and G-protein-coupled receptors in the context of neuromodulation. First, we will give a brief overview of each chemogenetic approach as well as in vitro/in vivo applications, then we will list their strengths and weaknesses. Finally, we will provide tips for ligand application in each case.Each technology has specific limitations that make them more or less suitable for different applications in neuroscience although we will focus mainly on the most commonly used and versatile family named designer receptors exclusively activated by designer drugs or DREADDs. We here describe the most common cases where these can be implemented and provide tips on how and where these technologies can be applied in the field of neuroscience.</p>},
  author       = {Aldrin-Kirk, Patrick and Björklund, Tomas},
  issn         = {1940-6029},
  keyword      = {Allatostatin,Chemogenetics,DREADD,Engineered receptors,G-Protein-coupled receptor,Gene therapy,GluCl,Ligand,Ligand-gated ion channel,Neuronal modulation,PSAM/PSEM,TRPV1},
  language     = {eng},
  month        = {01},
  pages        = {59--87},
  publisher    = {Springer},
  series       = {Methods in molecular biology (Clifton, N.J.)},
  title        = {Practical Considerations for the Use of DREADD and Other Chemogenetic Receptors to Regulate Neuronal Activity in the Mammalian Brain},
  url          = {http://dx.doi.org/10.1007/978-1-4939-9065-8_4},
  volume       = {1937},
  year         = {2019},
}