Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16
(2017) In Separation and Purification Technology 172. p.1-6- Abstract
In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB)... (More)
In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB) production that is more ecofriendly.
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- author
- Kunasundari, Balakrishnan ; Rodriguez Arza, Carlos LU ; Maurer, Frans H J LU ; Murugaiyah, Vikneswaran ; Kaur, Gurjeet and Sudesh, Kumar
- organization
- publishing date
- 2017-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cupriavidus necator H16, PHA, Poly(3-hydroxybutyrate), Recovery
- in
- Separation and Purification Technology
- volume
- 172
- pages
- 6 pages
- publisher
- Elsevier
- external identifiers
-
- wos:000385602300001
- scopus:84982733906
- ISSN
- 1383-5866
- DOI
- 10.1016/j.seppur.2016.07.043
- language
- English
- LU publication?
- yes
- id
- 7d188ea7-cda6-4b20-8ae3-3b7962617b8e
- date added to LUP
- 2016-10-19 10:11:39
- date last changed
- 2025-01-12 13:27:54
@article{7d188ea7-cda6-4b20-8ae3-3b7962617b8e,
abstract = {{<p>In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB) production that is more ecofriendly.</p>}},
author = {{Kunasundari, Balakrishnan and Rodriguez Arza, Carlos and Maurer, Frans H J and Murugaiyah, Vikneswaran and Kaur, Gurjeet and Sudesh, Kumar}},
issn = {{1383-5866}},
keywords = {{Cupriavidus necator H16; PHA; Poly(3-hydroxybutyrate); Recovery}},
language = {{eng}},
month = {{01}},
pages = {{1--6}},
publisher = {{Elsevier}},
series = {{Separation and Purification Technology}},
title = {{Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16}},
url = {{http://dx.doi.org/10.1016/j.seppur.2016.07.043}},
doi = {{10.1016/j.seppur.2016.07.043}},
volume = {{172}},
year = {{2017}},
}