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Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16

Kunasundari, Balakrishnan; Rodriguez Arza, Carlos LU ; Maurer, Frans H J LU ; Murugaiyah, Vikneswaran; Kaur, Gurjeet and Sudesh, Kumar (2017) In Separation and Purification Technology 172. p.1-6
Abstract

In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB)... (More)

In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB) production that is more ecofriendly.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cupriavidus necator H16, PHA, Poly(3-hydroxybutyrate), Recovery
in
Separation and Purification Technology
volume
172
pages
6 pages
publisher
Elsevier
external identifiers
  • scopus:84982733906
  • wos:000385602300001
ISSN
1383-5866
DOI
10.1016/j.seppur.2016.07.043
language
English
LU publication?
yes
id
7d188ea7-cda6-4b20-8ae3-3b7962617b8e
date added to LUP
2016-10-19 10:11:39
date last changed
2018-01-07 11:31:28
@article{7d188ea7-cda6-4b20-8ae3-3b7962617b8e,
  abstract     = {<p>In the present study, we observed that laboratory rats readily consumed lyophilized cells of Cupriavidus necator H16 cultivated using palm oil containing 39 wt% poly(3-hydroxybutyrate) [P(3HB)] as sole diet source. The test animals excreted whitish fecal pellets containing 82–97 wt% P(3HB). The remaining impurities could be easily removed by washing the pellets with water and/or low concentrations of detergent, which resulted in P(3HB) granules of a high purity. The molecular masses and thermal properties of P(3HB) obtained by this method were almost similar to P(3HB) extracted from bacterial cells using chloroform. The method reported here is simple and eliminates the need for solvents and strong chemicals, thus resulting in P(3HB) production that is more ecofriendly.</p>},
  author       = {Kunasundari, Balakrishnan and Rodriguez Arza, Carlos and Maurer, Frans H J and Murugaiyah, Vikneswaran and Kaur, Gurjeet and Sudesh, Kumar},
  issn         = {1383-5866},
  keyword      = {Cupriavidus necator H16,PHA,Poly(3-hydroxybutyrate),Recovery},
  language     = {eng},
  month        = {01},
  pages        = {1--6},
  publisher    = {Elsevier},
  series       = {Separation and Purification Technology},
  title        = {Biological recovery and properties of poly(3-hydroxybutyrate) from Cupriavidus necator H16},
  url          = {http://dx.doi.org/10.1016/j.seppur.2016.07.043},
  volume       = {172},
  year         = {2017},
}