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T Cell Modulation of Intimal Thickening After Vascular Injury. The Bimodal Role of IFN-{gamma} in Immune Deficiency.

Dimayuga, Paul C ; Li, Hongyan ; Chyu, Kuang-Yuh ; Nordin Fredrikson, Gunilla LU ; Nilsson, Jan LU ; Fishbein, Michael C ; Shah, Prediman K and Cercek, Bojan (2005) In Arteriosclerosis, Thrombosis and Vascular Biology 25(Oct 13). p.2528-2534
Abstract
Background - Immune deficiency results in exuberant intimal thickening after arterial injury. The mechanisms involved are not well defined. We investigated the role of T cells and IFN-gamma in the response to injury in normal and immune-deficient Rag-1KO mice. Methods and Results - Carotid arterial injury was induced in wild-type (WT), Rag-1KO mice, and Rag-1KO mice reconstituted with T cell-enriched splenocytes. The exuberant intimal thickening in Rag-1KO mice compared with WT mice 21 days after injury was reduced by T cell transfer (P < 0.01). Exogenous IFN-gamma starting on the day of injury inhibited intimal thickening in Rag-1KO mice. However, antibody neutralization of endogenous IFN-gamma in Rag-1KO mice starting 7 days after... (More)
Background - Immune deficiency results in exuberant intimal thickening after arterial injury. The mechanisms involved are not well defined. We investigated the role of T cells and IFN-gamma in the response to injury in normal and immune-deficient Rag-1KO mice. Methods and Results - Carotid arterial injury was induced in wild-type (WT), Rag-1KO mice, and Rag-1KO mice reconstituted with T cell-enriched splenocytes. The exuberant intimal thickening in Rag-1KO mice compared with WT mice 21 days after injury was reduced by T cell transfer (P < 0.01). Exogenous IFN-gamma starting on the day of injury inhibited intimal thickening in Rag-1KO mice. However, antibody neutralization of endogenous IFN-gamma in Rag-1KO mice starting 7 days after injury decreased intimal thickening, indicating that late presence of IFN-gamma promoted intimal thickening in Rag-1KO mice. Results further suggest that the effect of late IFN-gamma in Rag-1KO mice is mediated in part by increased IRF-1 and iNOS expression, coupled with low SOCS1 expression. Conclusion - T cells inhibit intimal thickening in the early stages of the response to injury through basal IFN-gamma secretion. In the Rag-1KO mice, late IFN-gamma expression promotes intimal thickening. These findings add novel insight to conditions of immune deficiency that affect intimal thickening. (Less)
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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
IFN-gamma, immune deficiency, mice, intimal thickening, Rag-1KO, lymphocytes
in
Arteriosclerosis, Thrombosis and Vascular Biology
volume
25
issue
Oct 13
pages
2528 - 2534
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000233461500017
  • pmid:16224059
  • scopus:33644802641
  • pmid:16224059
ISSN
1524-4636
DOI
10.1161/01.ATV.0000190606.41121.00
language
English
LU publication?
yes
id
7d1dc936-c0f8-4bb0-be9d-3b85e8e852a5 (old id 144650)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16224059&dopt=Abstract
date added to LUP
2016-04-01 12:15:17
date last changed
2022-01-27 01:06:24
@article{7d1dc936-c0f8-4bb0-be9d-3b85e8e852a5,
  abstract     = {{Background - Immune deficiency results in exuberant intimal thickening after arterial injury. The mechanisms involved are not well defined. We investigated the role of T cells and IFN-gamma in the response to injury in normal and immune-deficient Rag-1KO mice. Methods and Results - Carotid arterial injury was induced in wild-type (WT), Rag-1KO mice, and Rag-1KO mice reconstituted with T cell-enriched splenocytes. The exuberant intimal thickening in Rag-1KO mice compared with WT mice 21 days after injury was reduced by T cell transfer (P &lt; 0.01). Exogenous IFN-gamma starting on the day of injury inhibited intimal thickening in Rag-1KO mice. However, antibody neutralization of endogenous IFN-gamma in Rag-1KO mice starting 7 days after injury decreased intimal thickening, indicating that late presence of IFN-gamma promoted intimal thickening in Rag-1KO mice. Results further suggest that the effect of late IFN-gamma in Rag-1KO mice is mediated in part by increased IRF-1 and iNOS expression, coupled with low SOCS1 expression. Conclusion - T cells inhibit intimal thickening in the early stages of the response to injury through basal IFN-gamma secretion. In the Rag-1KO mice, late IFN-gamma expression promotes intimal thickening. These findings add novel insight to conditions of immune deficiency that affect intimal thickening.}},
  author       = {{Dimayuga, Paul C and Li, Hongyan and Chyu, Kuang-Yuh and Nordin Fredrikson, Gunilla and Nilsson, Jan and Fishbein, Michael C and Shah, Prediman K and Cercek, Bojan}},
  issn         = {{1524-4636}},
  keywords     = {{IFN-gamma; immune deficiency; mice; intimal thickening; Rag-1KO; lymphocytes}},
  language     = {{eng}},
  number       = {{Oct 13}},
  pages        = {{2528--2534}},
  publisher    = {{Lippincott Williams & Wilkins}},
  series       = {{Arteriosclerosis, Thrombosis and Vascular Biology}},
  title        = {{T Cell Modulation of Intimal Thickening After Vascular Injury. The Bimodal Role of IFN-{gamma} in Immune Deficiency.}},
  url          = {{http://dx.doi.org/10.1161/01.ATV.0000190606.41121.00}},
  doi          = {{10.1161/01.ATV.0000190606.41121.00}},
  volume       = {{25}},
  year         = {{2005}},
}