Diverse transcription factors are involved in the quantitative regulation of transcriptional activation of κ promoters
Bemark, Mats LU
and Leanderson, Tomas
LU
(1997)
In European Journal of Immunology
27(6).
p.1308-1318
- Abstract
Immunoglobulin κ promoters show sequence divergence but conserved function between different subgroups. Here we show that three separate 5' elements are required for synergistic stimulation of transcription with the decamer in a κ promoter. These sites are a 5' E-box, a 3' AT-rich region in the pentadecamer (pd) element, and the κ-Y element. Elf-1 is a novel κ-Y element ligand induced upon mitogenic stimulation of resting B lymphocytes. Furthermore, the 5' E2A-like E-box in the pd element could be substituted by an upstream stimulatory factor motif with conservation of function. Thus, the synergistic activation requirements of κ transcription is strictly dependent on the quantitative presence of transcription factor-binding motifs 5' of... (More)
Immunoglobulin κ promoters show sequence divergence but conserved function between different subgroups. Here we show that three separate 5' elements are required for synergistic stimulation of transcription with the decamer in a κ promoter. These sites are a 5' E-box, a 3' AT-rich region in the pentadecamer (pd) element, and the κ-Y element. Elf-1 is a novel κ-Y element ligand induced upon mitogenic stimulation of resting B lymphocytes. Furthermore, the 5' E2A-like E-box in the pd element could be substituted by an upstream stimulatory factor motif with conservation of function. Thus, the synergistic activation requirements of κ transcription is strictly dependent on the quantitative presence of transcription factor-binding motifs 5' of the decamer, but these differ qualitatively in that they may bind an array of proteins with conserved function.
(Less)
- author
- Bemark, Mats
LU
and Leanderson, Tomas
LU
- organization
- publishing date
- 1997-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- κ promoter, Elf-1, Oct protein, Transcription
- in
- European Journal of Immunology
- volume
- 27
- issue
- 6
- pages
- 1308 - 1318
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:9209478
- scopus:0030989911
- ISSN
- 0014-2980
- DOI
- 10.1002/eji.1830270603
- language
- English
- LU publication?
- yes
- id
- 7d4ffaa2-e690-4067-91b5-970b8d84cb6f
- date added to LUP
- 2023-12-06 17:10:29
- date last changed
- 2024-01-09 09:52:30
@article{7d4ffaa2-e690-4067-91b5-970b8d84cb6f,
abstract = {{<p>Immunoglobulin κ promoters show sequence divergence but conserved function between different subgroups. Here we show that three separate 5' elements are required for synergistic stimulation of transcription with the decamer in a κ promoter. These sites are a 5' E-box, a 3' AT-rich region in the pentadecamer (pd) element, and the κ-Y element. Elf-1 is a novel κ-Y element ligand induced upon mitogenic stimulation of resting B lymphocytes. Furthermore, the 5' E2A-like E-box in the pd element could be substituted by an upstream stimulatory factor motif with conservation of function. Thus, the synergistic activation requirements of κ transcription is strictly dependent on the quantitative presence of transcription factor-binding motifs 5' of the decamer, but these differ qualitatively in that they may bind an array of proteins with conserved function.</p>}},
author = {{Bemark, Mats and Leanderson, Tomas}},
issn = {{0014-2980}},
keywords = {{κ promoter; Elf-1; Oct protein; Transcription}},
language = {{eng}},
number = {{6}},
pages = {{1308--1318}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{Diverse transcription factors are involved in the quantitative regulation of transcriptional activation of κ promoters}},
url = {{http://dx.doi.org/10.1002/eji.1830270603}},
doi = {{10.1002/eji.1830270603}},
volume = {{27}},
year = {{1997}},
}