Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gamma delta T-cell ligand T10
(2004) In Acta Crystallographica. Section D: Biological Crystallography 60(4). p.656-664- Abstract
- T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 Angstrom resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a=78.2, b=70.0, c=139.2 Angstrom, beta=106.8degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alphasimilar or equal to0.46. Native Patterson... (More)
- T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 Angstrom resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a=78.2, b=70.0, c=139.2 Angstrom, beta=106.8degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alphasimilar or equal to0.46. Native Patterson analyses pointed to the presence of pseudo-translation among the four molecules present in the asymmetric unit. Data analysis, structure determination and model refinement are discussed. (Less)
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https://lup.lub.lu.se/record/140805
- author
- Rudolph, MG ; Wingren, Christer LU ; Crowley, MP ; Chien, YH and Wilson, IA
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta Crystallographica. Section D: Biological Crystallography
- volume
- 60
- issue
- 4
- pages
- 656 - 664
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- scopus:7944237130
- pmid:15039553
- ISSN
- 1399-0047
- DOI
- 10.1107/S0907444904002239
- language
- English
- LU publication?
- yes
- id
- 7d6437d3-b70b-42c2-b1da-df585fc20fe1 (old id 140805)
- date added to LUP
- 2016-04-01 15:49:55
- date last changed
- 2022-01-28 07:22:26
@article{7d6437d3-b70b-42c2-b1da-df585fc20fe1,
abstract = {{T10 is a non-classical class Ib-like major histocompatibility complex (MHC) cell-surface antigen which binds directly to certain gammadelta T-cell receptors in the absence of any exogenous and endogenous ligands, such as peculiar lipids or glycolipids. The crystal structure at 2.5 Angstrom resolution of murine T10 was determined by molecular replacement using data from an almost perfectly twinned monoclinic crystal. The space group is P2(1), with unit-cell parameters a=78.2, b=70.0, c=139.2 Angstrom, beta=106.8degrees. Self-rotation function analysis and various intensity statistics revealed the presence of pseudo-merohedral twinning, but these tests underestimated the true twin fraction of alphasimilar or equal to0.46. Native Patterson analyses pointed to the presence of pseudo-translation among the four molecules present in the asymmetric unit. Data analysis, structure determination and model refinement are discussed.}},
author = {{Rudolph, MG and Wingren, Christer and Crowley, MP and Chien, YH and Wilson, IA}},
issn = {{1399-0047}},
language = {{eng}},
number = {{4}},
pages = {{656--664}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Acta Crystallographica. Section D: Biological Crystallography}},
title = {{Combined pseudo-merohedral twinning, non-crystallographic symmetry and pseudo-translation in a monoclinic crystal form of the gamma delta T-cell ligand T10}},
url = {{http://dx.doi.org/10.1107/S0907444904002239}},
doi = {{10.1107/S0907444904002239}},
volume = {{60}},
year = {{2004}},
}