Molecularly imprinted monodisperse microspheres for competitive radioassay
(1999) In Analytical Communications 36(2). p.35-38- Abstract
In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micronscale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the 'classical' particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7da154d4-e5f2-47eb-bbb2-1b10f510494a
- author
- Ye, Lei LU ; Cormack, Peter A.G. and Mosbach, Klaus LU
- organization
- publishing date
- 1999-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Communications
- volume
- 36
- issue
- 2
- pages
- 4 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:0002791791
- ISSN
- 1359-7337
- DOI
- 10.1039/a809014i
- language
- English
- LU publication?
- yes
- id
- 7da154d4-e5f2-47eb-bbb2-1b10f510494a
- date added to LUP
- 2019-06-18 09:04:58
- date last changed
- 2022-04-18 07:06:22
@article{7da154d4-e5f2-47eb-bbb2-1b10f510494a, abstract = {{<p>In the present study, molecularly imprinted affinity sorbents against theophylline and 17β-estradiol are synthesised via precipitation polymerisation, a synthetic method that yields monodisperse, spherical polymer particles in the micronscale range, quickly, cleanly and in good yield. The specific binding sites that are created during the imprinting process are analysed via radioligand binding analysis. The molecularly imprinted microspheres are highly specific and have higher load capacities compared to the 'classical' particles obtained by grinding the imprinted monolith. The successful imprinting against model compounds with very different hydrophobicities demonstrates the generality of the current simple approach.</p>}}, author = {{Ye, Lei and Cormack, Peter A.G. and Mosbach, Klaus}}, issn = {{1359-7337}}, language = {{eng}}, month = {{01}}, number = {{2}}, pages = {{35--38}}, publisher = {{Royal Society of Chemistry}}, series = {{Analytical Communications}}, title = {{Molecularly imprinted monodisperse microspheres for competitive radioassay}}, url = {{http://dx.doi.org/10.1039/a809014i}}, doi = {{10.1039/a809014i}}, volume = {{36}}, year = {{1999}}, }