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Human Non-Hematopoietic CD271pos/CD140alow/neg Bone Marrow Stroma Cells Fulfill Stringent Stem Cell Criteria in Serial Transplantations

Ghazanfari, Roshanak LU ; Li, Hongzhe LU ; Zacharaki, Dimitra LU ; Lim, Hooi Ching LU and Scheding, Stefan LU (2016) In Stem Cells and Development 25(21). p.1652-1658
Abstract

Human bone marrow contains a population of non-hematopoietic stromal stem/progenitor cells (BMSCs), which play a central role for bone marrow stroma and the hematopoietic microenvironment. However, the precise characteristics and potential stem cell properties of defined BMSC populations have not yet been thoroughly investigated. Using standard adherent colony-forming unit fibroblast (CFU-F) assays, we have previously shown that BMSCs were highly enriched in the nonhematopoietic CD271pos/CD140alow/neg fraction of normal adult human bone marrow. In this study, we demonstrate that prospectively isolated CD271pos/CD140alow/neg BMSCs expressed high levels of hematopoiesis supporting genes and... (More)

Human bone marrow contains a population of non-hematopoietic stromal stem/progenitor cells (BMSCs), which play a central role for bone marrow stroma and the hematopoietic microenvironment. However, the precise characteristics and potential stem cell properties of defined BMSC populations have not yet been thoroughly investigated. Using standard adherent colony-forming unit fibroblast (CFU-F) assays, we have previously shown that BMSCs were highly enriched in the nonhematopoietic CD271pos/CD140alow/neg fraction of normal adult human bone marrow. In this study, we demonstrate that prospectively isolated CD271pos/CD140alow/neg BMSCs expressed high levels of hematopoiesis supporting genes and signature mesenchymal and multipotency genes on a single cell basis. Furthermore, CD271pos/CD140alow/neg BMSCs gave rise to non-adherent sphere colonies (mesenspheres) with typical surface marker profile and trilineage in vitro differentiation potential. Importantly, serial transplantations of CD271pos/CD140alow/neg BMSC-derived mesenspheres (single cell and bulk) into immunodeficient NOD scid gamma (NSG) mice showed increased mesensphere numbers and full differentiation potential after both primary and secondary transplantations. In contrast, BMSC self-renewal potential decreased under standard adherent culture conditions. These data therefore indicate that CD271pos/CD140alow/neg BMSCs represent a population of primary stem cells with MSC phenotype and sphere-forming capacity that fulfill stringent functional stem cell criteria in vivo in a serial transplantation setting.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
BMSC, bone marrow stromal cells, mesenspheres, self-renewal, serial transplantation, stem cells
in
Stem Cells and Development
volume
25
issue
21
pages
7 pages
publisher
Mary Ann Liebert, Inc.
external identifiers
  • scopus:84992709243
  • wos:000387035800004
ISSN
1547-3287
DOI
10.1089/scd.2016.0169
language
English
LU publication?
yes
id
7da4ff00-4d09-4357-85e3-2414974044e5
date added to LUP
2016-11-14 09:25:27
date last changed
2017-10-01 05:25:46
@article{7da4ff00-4d09-4357-85e3-2414974044e5,
  abstract     = {<p>Human bone marrow contains a population of non-hematopoietic stromal stem/progenitor cells (BMSCs), which play a central role for bone marrow stroma and the hematopoietic microenvironment. However, the precise characteristics and potential stem cell properties of defined BMSC populations have not yet been thoroughly investigated. Using standard adherent colony-forming unit fibroblast (CFU-F) assays, we have previously shown that BMSCs were highly enriched in the nonhematopoietic CD271<sup>pos</sup>/CD140a<sup>low/neg</sup> fraction of normal adult human bone marrow. In this study, we demonstrate that prospectively isolated CD271<sup>pos</sup>/CD140a<sup>low/neg</sup> BMSCs expressed high levels of hematopoiesis supporting genes and signature mesenchymal and multipotency genes on a single cell basis. Furthermore, CD271<sup>pos</sup>/CD140a<sup>low/neg</sup> BMSCs gave rise to non-adherent sphere colonies (mesenspheres) with typical surface marker profile and trilineage in vitro differentiation potential. Importantly, serial transplantations of CD271<sup>pos</sup>/CD140a<sup>low/neg</sup> BMSC-derived mesenspheres (single cell and bulk) into immunodeficient NOD scid gamma (NSG) mice showed increased mesensphere numbers and full differentiation potential after both primary and secondary transplantations. In contrast, BMSC self-renewal potential decreased under standard adherent culture conditions. These data therefore indicate that CD271<sup>pos</sup>/CD140a<sup>low/neg</sup> BMSCs represent a population of primary stem cells with MSC phenotype and sphere-forming capacity that fulfill stringent functional stem cell criteria in vivo in a serial transplantation setting.</p>},
  author       = {Ghazanfari, Roshanak and Li, Hongzhe and Zacharaki, Dimitra and Lim, Hooi Ching and Scheding, Stefan},
  issn         = {1547-3287},
  keyword      = {BMSC,bone marrow stromal cells,mesenspheres,self-renewal,serial transplantation,stem cells},
  language     = {eng},
  month        = {11},
  number       = {21},
  pages        = {1652--1658},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Stem Cells and Development},
  title        = {Human Non-Hematopoietic CD271<sup>pos</sup>/CD140a<sup>low/neg</sup> Bone Marrow Stroma Cells Fulfill Stringent Stem Cell Criteria in Serial Transplantations},
  url          = {http://dx.doi.org/10.1089/scd.2016.0169},
  volume       = {25},
  year         = {2016},
}