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Enhanced human T cell expansion with inverse opal hydrogels†

Santos, Fabião ; Valderas Gutiérrez, Julia LU orcid ; Pérez del Río, Eduardo ; Castellote-Borrell, Miquel ; Rodriguez Rodriguez, Xavier ; Veciana, Jaume ; Ratera, Imma and Guasch, Judith (2022) In Biomaterials Science
Abstract
Advanced personalized immunotherapies still have to overcome several biomedical and technical limitations before they become a routine cancer treatment in spite of recent achievements. In adoptive cell therapy (ACT), the capacity to obtain adequate numbers of therapeutic T cells in the patients following ex vivo treatment should be improved. Moreover, the time and costs to produce these T cells should be reduced. In this work, inverse opal (IOPAL) 3D hydrogels consisting of poly(ethylene) glycol (PEG) covalently combined with heparin were engineered to resemble the environment of lymph nodes, where T cells get activated and proliferate. The introduction of an IOPAL strategy allowed a precise control on the porosity of the hydrogels,... (More)
Advanced personalized immunotherapies still have to overcome several biomedical and technical limitations before they become a routine cancer treatment in spite of recent achievements. In adoptive cell therapy (ACT), the capacity to obtain adequate numbers of therapeutic T cells in the patients following ex vivo treatment should be improved. Moreover, the time and costs to produce these T cells should be reduced. In this work, inverse opal (IOPAL) 3D hydrogels consisting of poly(ethylene) glycol (PEG) covalently combined with heparin were engineered to resemble the environment of lymph nodes, where T cells get activated and proliferate. The introduction of an IOPAL strategy allowed a precise control on the porosity of the hydrogels, providing an increase in the proliferation of primary human CD4+ T cells, when compared with state-of-the-art expansion systems. Additionally, the IOPAL hydrogels also showed a superior expansion compared to hydrogels with the same composition, but without the predetermined pore structure. In summary, we have shown the beneficial effect of having an IOPAL architecture in our 3D hydrogels to help achieving large numbers of cells, while maintaining the desired selected phenotypes required for ACT. (Less)
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author
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publishing date
type
Contribution to journal
publication status
published
subject
in
Biomaterials Science
publisher
Royal Society of Chemistry
external identifiers
  • scopus:85131969250
ISSN
2047-4830
DOI
10.1039/D2BM00486K
language
English
LU publication?
no
id
7dab5ef7-6333-4a4a-ac5f-d5f9ed500760
date added to LUP
2022-06-06 14:14:54
date last changed
2023-07-06 04:08:49
@article{7dab5ef7-6333-4a4a-ac5f-d5f9ed500760,
  abstract     = {{Advanced personalized immunotherapies still have to overcome several biomedical and technical limitations before they become a routine cancer treatment in spite of recent achievements. In adoptive cell therapy (ACT), the capacity to obtain adequate numbers of therapeutic T cells in the patients following ex vivo treatment should be improved. Moreover, the time and costs to produce these T cells should be reduced. In this work, inverse opal (IOPAL) 3D hydrogels consisting of poly(ethylene) glycol (PEG) covalently combined with heparin were engineered to resemble the environment of lymph nodes, where T cells get activated and proliferate. The introduction of an IOPAL strategy allowed a precise control on the porosity of the hydrogels, providing an increase in the proliferation of primary human CD4+ T cells, when compared with state-of-the-art expansion systems. Additionally, the IOPAL hydrogels also showed a superior expansion compared to hydrogels with the same composition, but without the predetermined pore structure. In summary, we have shown the beneficial effect of having an IOPAL architecture in our 3D hydrogels to help achieving large numbers of cells, while maintaining the desired selected phenotypes required for ACT.}},
  author       = {{Santos, Fabião and Valderas Gutiérrez, Julia and Pérez del Río, Eduardo and Castellote-Borrell, Miquel and Rodriguez Rodriguez, Xavier and Veciana, Jaume and Ratera, Imma and Guasch, Judith}},
  issn         = {{2047-4830}},
  language     = {{eng}},
  month        = {{06}},
  publisher    = {{Royal Society of Chemistry}},
  series       = {{Biomaterials Science}},
  title        = {{Enhanced human T cell expansion with inverse opal hydrogels†}},
  url          = {{http://dx.doi.org/10.1039/D2BM00486K}},
  doi          = {{10.1039/D2BM00486K}},
  year         = {{2022}},
}