An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes
(1995) In Journal of Immunological Methods 185(1). p.1-8- Abstract
Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.
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- author
- Lollike, K ; Sørensen, O LU ; Bundgaard, J R ; Segal, A W ; Boyhan, A and Borregaard, N
- publishing date
- 1995-09-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Antibody Specificity, Calcium-Binding Proteins/analysis, Enzyme-Linked Immunosorbent Assay, Humans, Leukocytes, Mononuclear/chemistry, Neutrophils/chemistry, Recombinant Proteins
- in
- Journal of Immunological Methods
- volume
- 185
- issue
- 1
- pages
- 8 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:7665891
- scopus:0029087737
- ISSN
- 0022-1759
- DOI
- 10.1016/0022-1759(95)00099-v
- language
- English
- LU publication?
- no
- id
- 7db97644-bf47-442e-af6a-01ff78c31be5
- date added to LUP
- 2020-11-05 13:40:06
- date last changed
- 2024-01-02 21:02:50
@article{7db97644-bf47-442e-af6a-01ff78c31be5, abstract = {{<p>Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.</p>}}, author = {{Lollike, K and Sørensen, O and Bundgaard, J R and Segal, A W and Boyhan, A and Borregaard, N}}, issn = {{0022-1759}}, keywords = {{Antibody Specificity; Calcium-Binding Proteins/analysis; Enzyme-Linked Immunosorbent Assay; Humans; Leukocytes, Mononuclear/chemistry; Neutrophils/chemistry; Recombinant Proteins}}, language = {{eng}}, month = {{09}}, number = {{1}}, pages = {{1--8}}, publisher = {{Elsevier}}, series = {{Journal of Immunological Methods}}, title = {{An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes}}, url = {{http://dx.doi.org/10.1016/0022-1759(95)00099-v}}, doi = {{10.1016/0022-1759(95)00099-v}}, volume = {{185}}, year = {{1995}}, }