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An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes

Lollike, K ; Sørensen, O LU ; Bundgaard, J R ; Segal, A W ; Boyhan, A and Borregaard, N (1995) In Journal of Immunological Methods 185(1). p.1-8
Abstract

Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Antibody Specificity, Calcium-Binding Proteins/analysis, Enzyme-Linked Immunosorbent Assay, Humans, Leukocytes, Mononuclear/chemistry, Neutrophils/chemistry, Recombinant Proteins
in
Journal of Immunological Methods
volume
185
issue
1
pages
8 pages
publisher
Elsevier
external identifiers
  • pmid:7665891
  • scopus:0029087737
ISSN
0022-1759
DOI
10.1016/0022-1759(95)00099-v
language
English
LU publication?
no
id
7db97644-bf47-442e-af6a-01ff78c31be5
date added to LUP
2020-11-05 13:40:06
date last changed
2024-01-02 21:02:50
@article{7db97644-bf47-442e-af6a-01ff78c31be5,
  abstract     = {{<p>Grancalcin is a newly discovered cytosolic calcium-binding protein, belonging to the group of EF-hand proteins. Grancalcin is specifically associated with cells originating in the bone marrow. Grancalcin binds reversibly to secretory vesicles and plasma membranes in human neutrophils and might therefore play a role in the regulation of vesicle/granule exocytosis. We describe here the production of recombinant grancalcin, the generation of antibodies to the protein and the development of a specific, accurate and sensitive ELISA for the detection of human leukocytic grancalcin. This ELISA may be useful for monitoring leukocyte infiltration into tissues.</p>}},
  author       = {{Lollike, K and Sørensen, O and Bundgaard, J R and Segal, A W and Boyhan, A and Borregaard, N}},
  issn         = {{0022-1759}},
  keywords     = {{Antibody Specificity; Calcium-Binding Proteins/analysis; Enzyme-Linked Immunosorbent Assay; Humans; Leukocytes, Mononuclear/chemistry; Neutrophils/chemistry; Recombinant Proteins}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{1}},
  pages        = {{1--8}},
  publisher    = {{Elsevier}},
  series       = {{Journal of Immunological Methods}},
  title        = {{An ELISA for grancalcin, a novel cytosolic calcium-binding protein present in leukocytes}},
  url          = {{http://dx.doi.org/10.1016/0022-1759(95)00099-v}},
  doi          = {{10.1016/0022-1759(95)00099-v}},
  volume       = {{185}},
  year         = {{1995}},
}