Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation
(2023) In STAR Protocols 4(2).- Abstract
High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis. We describe the use of a notch signaling inhibitor to generate homogeneous neuronal populations at densities amenable to individual neurite identification. We detail neurite morphology assessment via measuring multiple parameters including neurite length, branches, roots, segments and extremities, and neuron maturation.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7dd4f772-18cd-4df0-b975-09538f27e5e5
- author
- Wali, Gautam ; Li, Yan ; Abu-Bonsrah, Dad ; Kirik, Deniz LU ; Parish, Clare L. and Sue, Carolyn M.
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell Biology, Cell culture, Cell Differentiation, Microscopy, Neuroscience, Stem Cells
- in
- STAR Protocols
- volume
- 4
- issue
- 2
- article number
- 102325
- publisher
- Cell Press
- external identifiers
-
- pmid:37300830
- scopus:85161319324
- ISSN
- 2666-1667
- DOI
- 10.1016/j.xpro.2023.102325
- language
- English
- LU publication?
- yes
- id
- 7dd4f772-18cd-4df0-b975-09538f27e5e5
- date added to LUP
- 2023-08-15 11:30:44
- date last changed
- 2024-04-20 01:36:03
@article{7dd4f772-18cd-4df0-b975-09538f27e5e5, abstract = {{<p>High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis. We describe the use of a notch signaling inhibitor to generate homogeneous neuronal populations at densities amenable to individual neurite identification. We detail neurite morphology assessment via measuring multiple parameters including neurite length, branches, roots, segments and extremities, and neuron maturation.</p>}}, author = {{Wali, Gautam and Li, Yan and Abu-Bonsrah, Dad and Kirik, Deniz and Parish, Clare L. and Sue, Carolyn M.}}, issn = {{2666-1667}}, keywords = {{Cell Biology; Cell culture; Cell Differentiation; Microscopy; Neuroscience; Stem Cells}}, language = {{eng}}, number = {{2}}, publisher = {{Cell Press}}, series = {{STAR Protocols}}, title = {{Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation}}, url = {{http://dx.doi.org/10.1016/j.xpro.2023.102325}}, doi = {{10.1016/j.xpro.2023.102325}}, volume = {{4}}, year = {{2023}}, }