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Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation

Wali, Gautam ; Li, Yan ; Abu-Bonsrah, Dad ; Kirik, Deniz LU ; Parish, Clare L. and Sue, Carolyn M. (2023) In STAR Protocols 4(2).
Abstract

High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis. We describe the use of a notch signaling inhibitor to generate homogeneous neuronal populations at densities amenable to individual neurite identification. We detail neurite morphology assessment via measuring multiple parameters including neurite length, branches, roots, segments and extremities, and neuron maturation.

Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell Biology, Cell culture, Cell Differentiation, Microscopy, Neuroscience, Stem Cells
in
STAR Protocols
volume
4
issue
2
article number
102325
publisher
Cell Press
external identifiers
  • pmid:37300830
  • scopus:85161319324
ISSN
2666-1667
DOI
10.1016/j.xpro.2023.102325
language
English
LU publication?
yes
id
7dd4f772-18cd-4df0-b975-09538f27e5e5
date added to LUP
2023-08-15 11:30:44
date last changed
2024-04-20 01:36:03
@article{7dd4f772-18cd-4df0-b975-09538f27e5e5,
  abstract     = {{<p>High-throughput imaging allows in vitro assessment of neuron morphology for screening populations under developmental, homeostatic, and/or disease conditions. Here, we present a protocol to differentiate cryopreserved human cortical neuronal progenitors into mature cortical neurons for high-throughput imaging analysis. We describe the use of a notch signaling inhibitor to generate homogeneous neuronal populations at densities amenable to individual neurite identification. We detail neurite morphology assessment via measuring multiple parameters including neurite length, branches, roots, segments and extremities, and neuron maturation.</p>}},
  author       = {{Wali, Gautam and Li, Yan and Abu-Bonsrah, Dad and Kirik, Deniz and Parish, Clare L. and Sue, Carolyn M.}},
  issn         = {{2666-1667}},
  keywords     = {{Cell Biology; Cell culture; Cell Differentiation; Microscopy; Neuroscience; Stem Cells}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{Cell Press}},
  series       = {{STAR Protocols}},
  title        = {{Generation of human-induced pluripotent-stem-cell-derived cortical neurons for high-throughput imaging of neurite morphology and neuron maturation}},
  url          = {{http://dx.doi.org/10.1016/j.xpro.2023.102325}},
  doi          = {{10.1016/j.xpro.2023.102325}},
  volume       = {{4}},
  year         = {{2023}},
}