Comparison of platelet immunity in patients with SLE and with ITP
(2000) In Transfusion Science 22(1-2). p.19-27- Abstract
Idiopathic thrombocytopenic purpura (ITP) is characterized by the development of a specific anti-platelet autoantibody immune response mediating the development of thrombocytopenia. Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of a wide variety of autoantibodies. In 15-20% of SLE cases, patients develop thrombocytopenia which appears to be autoimmune in nature (SLE-TP). To better understand the pathogenesis of the thrombocytopenia associated with SLE, we investigated the overlapping platelet and cellular immune features between SLE and ITP. Thirty-one patients with SLE, eight with SLE-TP, and 17 with ITP, were studied and compared to 60 healthy controls. We evaluated platelet-associated... (More)
Idiopathic thrombocytopenic purpura (ITP) is characterized by the development of a specific anti-platelet autoantibody immune response mediating the development of thrombocytopenia. Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of a wide variety of autoantibodies. In 15-20% of SLE cases, patients develop thrombocytopenia which appears to be autoimmune in nature (SLE-TP). To better understand the pathogenesis of the thrombocytopenia associated with SLE, we investigated the overlapping platelet and cellular immune features between SLE and ITP. Thirty-one patients with SLE, eight with SLE-TP, and 17 with ITP, were studied and compared to 60 healthy controls. We evaluated platelet-associated IgG, platelet microparticles, reticulated platelets, platelet HLA-DR expression, in vivo cytokine levels, lymphocyte proliferation, and the T lymphocyte anti-platelet immune response in these patients. Patients with SLE-TP and those with ITP had increased platelet-associated IgG, an increased percentage of platelet microparticles, a higher percentage of reticulated platelets and larger platelets, suggesting antibody-mediated platelet destruction and increased platelet production. More than 50% of patients with ITP had increased HLA-DR on their platelet surface whereas subjects with SLE-TP did not. Analysis of serum cytokines demonstrated increased levels of IL-10, IL-15 and TNF-alpha in patients with SLE, but in those with ITP, only increased levels of IL-15 were seen, no increases in any of these cytokines were observed in patients with in SLE-TP. The ability of lymphocytes to proliferate in response to phorbol myristate acetate (PMA) stimulation was increased in SLE-TP, but was normal in both SLE and ITP. Lymphocytes from subjects with ITP displayed an increased ability to proliferate on exposure to platelets, in contrast, those with SLE-TP did not. While the number of subjects evaluated with SLE-TP was small, these data reveal a number of differences in the immunopathogenesis between SLE-TP and ITP.
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- author
- Lazarus, A H ; Ellis, J ; Semple, J W LU ; Mody, M ; Crow, A R and Freedman, J
- publishing date
- 2000-04-20
- type
- Contribution to journal
- publication status
- published
- keywords
- Adult, Antigen-Presenting Cells/cytology, Blood Platelets/cytology, Cell Division/drug effects, Cytokines/blood, Cytological Techniques, Humans, Immunity, Lupus Erythematosus, Systemic/blood, Lymphocyte Activation, Lymphocytes/cytology, Mitogens/pharmacology, Platelet Count, Platelet Function Tests, Purpura, Thrombocytopenic, Idiopathic/blood
- in
- Transfusion Science
- volume
- 22
- issue
- 1-2
- pages
- 19 - 27
- publisher
- Pergamon Press Ltd.
- external identifiers
-
- scopus:0347899606
- pmid:10771375
- ISSN
- 0955-3886
- DOI
- 10.1016/s0955-3886(00)00004-7
- language
- English
- LU publication?
- no
- id
- 7de2a5a8-519b-4958-8902-53dcf3d76f54
- date added to LUP
- 2022-11-09 15:27:15
- date last changed
- 2024-01-03 18:43:37
@article{7de2a5a8-519b-4958-8902-53dcf3d76f54,
abstract = {{<p>Idiopathic thrombocytopenic purpura (ITP) is characterized by the development of a specific anti-platelet autoantibody immune response mediating the development of thrombocytopenia. Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the production of a wide variety of autoantibodies. In 15-20% of SLE cases, patients develop thrombocytopenia which appears to be autoimmune in nature (SLE-TP). To better understand the pathogenesis of the thrombocytopenia associated with SLE, we investigated the overlapping platelet and cellular immune features between SLE and ITP. Thirty-one patients with SLE, eight with SLE-TP, and 17 with ITP, were studied and compared to 60 healthy controls. We evaluated platelet-associated IgG, platelet microparticles, reticulated platelets, platelet HLA-DR expression, in vivo cytokine levels, lymphocyte proliferation, and the T lymphocyte anti-platelet immune response in these patients. Patients with SLE-TP and those with ITP had increased platelet-associated IgG, an increased percentage of platelet microparticles, a higher percentage of reticulated platelets and larger platelets, suggesting antibody-mediated platelet destruction and increased platelet production. More than 50% of patients with ITP had increased HLA-DR on their platelet surface whereas subjects with SLE-TP did not. Analysis of serum cytokines demonstrated increased levels of IL-10, IL-15 and TNF-alpha in patients with SLE, but in those with ITP, only increased levels of IL-15 were seen, no increases in any of these cytokines were observed in patients with in SLE-TP. The ability of lymphocytes to proliferate in response to phorbol myristate acetate (PMA) stimulation was increased in SLE-TP, but was normal in both SLE and ITP. Lymphocytes from subjects with ITP displayed an increased ability to proliferate on exposure to platelets, in contrast, those with SLE-TP did not. While the number of subjects evaluated with SLE-TP was small, these data reveal a number of differences in the immunopathogenesis between SLE-TP and ITP.</p>}},
author = {{Lazarus, A H and Ellis, J and Semple, J W and Mody, M and Crow, A R and Freedman, J}},
issn = {{0955-3886}},
keywords = {{Adult; Antigen-Presenting Cells/cytology; Blood Platelets/cytology; Cell Division/drug effects; Cytokines/blood; Cytological Techniques; Humans; Immunity; Lupus Erythematosus, Systemic/blood; Lymphocyte Activation; Lymphocytes/cytology; Mitogens/pharmacology; Platelet Count; Platelet Function Tests; Purpura, Thrombocytopenic, Idiopathic/blood}},
language = {{eng}},
month = {{04}},
number = {{1-2}},
pages = {{19--27}},
publisher = {{Pergamon Press Ltd.}},
series = {{Transfusion Science}},
title = {{Comparison of platelet immunity in patients with SLE and with ITP}},
url = {{http://dx.doi.org/10.1016/s0955-3886(00)00004-7}},
doi = {{10.1016/s0955-3886(00)00004-7}},
volume = {{22}},
year = {{2000}},
}