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Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells

Wang, Wei ; Di Nisio, Elena ; Licursi, Valerio ; Cacci, Emanuele LU ; Lupo, Giuseppe ; Kokaia, Zaal LU orcid ; Galanti, Sergio ; Degan, Paolo ; D’Angelo, Sara and Castagnola, Patrizio , et al. (2022) In Life 12(11).
Abstract

We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture... (More)

We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture conditions under normal gravity. These results suggest that reduced gravity counteracts the interactions of cells with the extracellular matrix, inducing morphological and transcriptional changes that can be observed in low-density cultures.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
focal adhesion, human neural stem progenitor cells, laminin, microgravity, space conditions
in
Life
volume
12
issue
11
article number
1827
publisher
MDPI AG
external identifiers
  • scopus:85141693372
  • pmid:36362982
ISSN
0024-3019
DOI
10.3390/life12111827
language
English
LU publication?
yes
id
7e0d839e-c3da-46bb-9923-c44a860be114
date added to LUP
2022-11-30 10:13:30
date last changed
2024-06-11 19:29:22
@article{7e0d839e-c3da-46bb-9923-c44a860be114,
  abstract     = {{<p>We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture conditions under normal gravity. These results suggest that reduced gravity counteracts the interactions of cells with the extracellular matrix, inducing morphological and transcriptional changes that can be observed in low-density cultures.</p>}},
  author       = {{Wang, Wei and Di Nisio, Elena and Licursi, Valerio and Cacci, Emanuele and Lupo, Giuseppe and Kokaia, Zaal and Galanti, Sergio and Degan, Paolo and D’Angelo, Sara and Castagnola, Patrizio and Tavella, Sara and Negri, Rodolfo}},
  issn         = {{0024-3019}},
  keywords     = {{focal adhesion; human neural stem progenitor cells; laminin; microgravity; space conditions}},
  language     = {{eng}},
  number       = {{11}},
  publisher    = {{MDPI AG}},
  series       = {{Life}},
  title        = {{Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells}},
  url          = {{http://dx.doi.org/10.3390/life12111827}},
  doi          = {{10.3390/life12111827}},
  volume       = {{12}},
  year         = {{2022}},
}