Activated Peyer′s patch B cells sample antigen directly from M cells in the subepithelial dome
Komban, Rathan Joy ; Strömberg, Anneli ; Biram, Adi ; Cervin, Jakob ; Lebrero-Fernández, Cristina ; Mabbott, Neil ; Yrlid, Ulf LU ; Shulman, Ziv ; Bemark, Mats LU
and Lycke, Nils
(2019)
In Nature Communications
10.
p.1-15
- Abstract
The germinal center (GC) reaction in Peyer′s patches (PP) requires continuous access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7− B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40% of antigen-specific SED B cells bind antigen within 2 h, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell-deficient mice, but is unperturbed in mice depleted of classical dendritic cells... (More)
The germinal center (GC) reaction in Peyer′s patches (PP) requires continuous access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6+CCR1+GL7− B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40% of antigen-specific SED B cells bind antigen within 2 h, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell-deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines.
(Less)
- author
- Komban, Rathan Joy
; Strömberg, Anneli
; Biram, Adi
; Cervin, Jakob
; Lebrero-Fernández, Cristina
; Mabbott, Neil
; Yrlid, Ulf
LU
; Shulman, Ziv
; Bemark, Mats
LU
and Lycke, Nils
- publishing date
- 2019-12-01
- type
- Contribution to journal
- publication status
- published
- in
- Nature Communications
- volume
- 10
- article number
- 2423
- pages
- 1 - 15
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:31160559
- scopus:85066610734
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-019-10144-w
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2019, The Author(s).
- id
- 7e14efc1-1e69-4e5f-ad01-6ee1461dd9a8
- date added to LUP
- 2023-12-06 17:18:11
- date last changed
- 2024-04-19 12:56:22
@article{7e14efc1-1e69-4e5f-ad01-6ee1461dd9a8,
abstract = {{<p>The germinal center (GC) reaction in Peyer′s patches (PP) requires continuous access to antigens, but how this is achieved is not known. Here we show that activated antigen-specific CCR6<sup>+</sup>CCR1<sup>+</sup>GL7<sup>−</sup> B cells make close contact with M cells in the subepithelial dome (SED). Using in situ photoactivation analysis of antigen-specific SED B cells, we find migration of cells towards the GC. Following antigen injection into ligated intestinal loops containing PPs, 40% of antigen-specific SED B cells bind antigen within 2 h, whereas unspecifc cells do not, indicating B cell-receptor involvment. Antigen-loading is not observed in M cell-deficient mice, but is unperturbed in mice depleted of classical dendritic cells (DC). Thus, we report a M cell-B cell antigen-specific transporting pathway in PP that is independent of DC. We propose that this antigen transporting pathway has a critical role in gut IgA responses, and should be taken into account when developing mucosal vaccines.</p>}},
author = {{Komban, Rathan Joy and Strömberg, Anneli and Biram, Adi and Cervin, Jakob and Lebrero-Fernández, Cristina and Mabbott, Neil and Yrlid, Ulf and Shulman, Ziv and Bemark, Mats and Lycke, Nils}},
issn = {{2041-1723}},
language = {{eng}},
month = {{12}},
pages = {{1--15}},
publisher = {{Nature Publishing Group}},
series = {{Nature Communications}},
title = {{Activated Peyer′s patch B cells sample antigen directly from M cells in the subepithelial dome}},
url = {{http://dx.doi.org/10.1038/s41467-019-10144-w}},
doi = {{10.1038/s41467-019-10144-w}},
volume = {{10}},
year = {{2019}},
}