Artificially controlled aggregation of proteins and targeting in hematopoietic cells
(2003) In Journal of Leukocyte Biology 74(5). p.800-809- Abstract
- The targeting mechanisms for granule proteins in hematopoietic cells are largely unknown. Aggregation is believed to be important for protein sorting-for-entry and sorting-by-retention in endocrine and neuroendocrine cells. We asked whether artificially induced multimerization/aggregation of chimeric proteins could affect their sorting in hematopoietic cells. A system was used that permits ligand-controlled intracellular oligomerization of hybrid proteins containing the FK506-binding protein (FKBP). The hybrid proteins ELA(FKBP)(3) with neutrophil elastase (ELA) and (FKBP*)(4)-FCS-hGH with a furin cleavage site (FCS) and human growth hormone (hGH) were expressed in the myeloblastic 32D and the rat basophilic leukemia (RBL-1) hematopoietic... (More)
- The targeting mechanisms for granule proteins in hematopoietic cells are largely unknown. Aggregation is believed to be important for protein sorting-for-entry and sorting-by-retention in endocrine and neuroendocrine cells. We asked whether artificially induced multimerization/aggregation of chimeric proteins could affect their sorting in hematopoietic cells. A system was used that permits ligand-controlled intracellular oligomerization of hybrid proteins containing the FK506-binding protein (FKBP). The hybrid proteins ELA(FKBP)(3) with neutrophil elastase (ELA) and (FKBP*)(4)-FCS-hGH with a furin cleavage site (FCS) and human growth hormone (hGH) were expressed in the myeloblastic 32D and the rat basophilic leukemia (RBL-1) hematopoietic cell lines. ELA alone is normally targeted to secretory lysosomes. However, the hybrid proteins and ligand-induced aggregates of them were constitutively secreted and not targeted. The hGH that was released at the FCS in (FKBP*)(4)-FCS-hGH was also constitutively secreted. We conclude that protein multimerization/aggregation per se is not enough to facilitate sorting-for-entry to secretory lysosomes in hematopoietic cells and that improperly folded proteins may be eliminated from sorting by constitutive secretion. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/292388
- author
- Rosén, Hanna LU ; Gao, Ying LU ; Johnsson, E and Olsson, Inge LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- secretary lysosomes, storage, multimerization, quality control, secretion
- in
- Journal of Leukocyte Biology
- volume
- 74
- issue
- 5
- pages
- 800 - 809
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000187392400021
- pmid:12960262
- scopus:0347991982
- pmid:12960262
- ISSN
- 1938-3673
- DOI
- 10.1189/jlb.0203066
- language
- English
- LU publication?
- yes
- id
- 7e29d3a5-06e9-4766-9d52-c066dbf871b5 (old id 292388)
- date added to LUP
- 2016-04-01 11:57:09
- date last changed
- 2022-01-26 20:38:27
@article{7e29d3a5-06e9-4766-9d52-c066dbf871b5, abstract = {{The targeting mechanisms for granule proteins in hematopoietic cells are largely unknown. Aggregation is believed to be important for protein sorting-for-entry and sorting-by-retention in endocrine and neuroendocrine cells. We asked whether artificially induced multimerization/aggregation of chimeric proteins could affect their sorting in hematopoietic cells. A system was used that permits ligand-controlled intracellular oligomerization of hybrid proteins containing the FK506-binding protein (FKBP). The hybrid proteins ELA(FKBP)(3) with neutrophil elastase (ELA) and (FKBP*)(4)-FCS-hGH with a furin cleavage site (FCS) and human growth hormone (hGH) were expressed in the myeloblastic 32D and the rat basophilic leukemia (RBL-1) hematopoietic cell lines. ELA alone is normally targeted to secretory lysosomes. However, the hybrid proteins and ligand-induced aggregates of them were constitutively secreted and not targeted. The hGH that was released at the FCS in (FKBP*)(4)-FCS-hGH was also constitutively secreted. We conclude that protein multimerization/aggregation per se is not enough to facilitate sorting-for-entry to secretory lysosomes in hematopoietic cells and that improperly folded proteins may be eliminated from sorting by constitutive secretion.}}, author = {{Rosén, Hanna and Gao, Ying and Johnsson, E and Olsson, Inge}}, issn = {{1938-3673}}, keywords = {{secretary lysosomes; storage; multimerization; quality control; secretion}}, language = {{eng}}, number = {{5}}, pages = {{800--809}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Leukocyte Biology}}, title = {{Artificially controlled aggregation of proteins and targeting in hematopoietic cells}}, url = {{http://dx.doi.org/10.1189/jlb.0203066}}, doi = {{10.1189/jlb.0203066}}, volume = {{74}}, year = {{2003}}, }