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Optimization of a Membraneless Glucose/Oxygen Enzymatic Fuel Cell Based on a Bioanode with High Coulombic Efficiency and Current Density

Shao, Minling; Zafar, MNadeem LU ; Falk, Magnus; Ludwig, Roland; Sygmund, Christoph; Peterbauer, Clemens K.; Guschin, Dmitrii A.; MacAodha, Domhnall; Conghaile, Peter O. and Leech, Donal, et al. (2013) In ChemPhysChem 14(10). p.2260-2269
Abstract
After initial testing and optimization of anode biocatalysts, a membraneless glucose/oxygen enzymatic biofuel cell possessing high coulombic efficiency and power output was fabricated and characterized. Agaricus meleagris (AmPDH) and flavodehydrogenase domains of various cellobiose dehydrogenases (DHCDH) were tested during the pre-screening. Myrothecium verrucaria adsorbed on graphite. Optimization showed that the current density for the mixed enzyme electrode could be further improved by using a genetically engineered variant of the non-glycosylated flavodehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophilus expressed in E. coli (ngDH(CtCDHC310Y)) with a high glucose-turnover rate in combination with an... (More)
After initial testing and optimization of anode biocatalysts, a membraneless glucose/oxygen enzymatic biofuel cell possessing high coulombic efficiency and power output was fabricated and characterized. Agaricus meleagris (AmPDH) and flavodehydrogenase domains of various cellobiose dehydrogenases (DHCDH) were tested during the pre-screening. Myrothecium verrucaria adsorbed on graphite. Optimization showed that the current density for the mixed enzyme electrode could be further improved by using a genetically engineered variant of the non-glycosylated flavodehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophilus expressed in E. coli (ngDH(CtCDHC310Y)) with a high glucose-turnover rate in combination with an Os-complex-modified redox polymer with a high concentration of Os complexes as well as a low-density graphite electrode. AmPDH/ngDH(CtCDHC310Y) anode showed not only a similar maximum voltage as with the biofuel cell based only on the ngDH(CtCDHC310Y) anode (0.55 V) but also a substantially improved maximum power output (20 Wcm(-2)) at 300 mV cell voltage in air-saturated physiological buffer. Most importantly, the estimated half-life of the mixed biofuel cell can reach up to 12 h, which is apparently longer than that of a biofuel cell in which the bioanode is based on only one single enzyme. (Less)
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published
subject
keywords
biofuel cells, cellobiose dehydrogenase, coulombic efficiency, electrochemistry, electron transfer
in
ChemPhysChem
volume
14
issue
10
pages
2260 - 2269
publisher
John Wiley & Sons
external identifiers
  • wos:000322236400034
  • scopus:84880945316
ISSN
1439-7641
DOI
10.1002/cphc.201300046
language
English
LU publication?
yes
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7e2c6842-bc1e-4b15-b5e7-53ddca1068b3 (old id 4112512)
date added to LUP
2013-10-28 11:08:58
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2019-02-03 03:14:15
@article{7e2c6842-bc1e-4b15-b5e7-53ddca1068b3,
  abstract     = {After initial testing and optimization of anode biocatalysts, a membraneless glucose/oxygen enzymatic biofuel cell possessing high coulombic efficiency and power output was fabricated and characterized. Agaricus meleagris (AmPDH) and flavodehydrogenase domains of various cellobiose dehydrogenases (DHCDH) were tested during the pre-screening. Myrothecium verrucaria adsorbed on graphite. Optimization showed that the current density for the mixed enzyme electrode could be further improved by using a genetically engineered variant of the non-glycosylated flavodehydrogenase domain of cellobiose dehydrogenase from Corynascus thermophilus expressed in E. coli (ngDH(CtCDHC310Y)) with a high glucose-turnover rate in combination with an Os-complex-modified redox polymer with a high concentration of Os complexes as well as a low-density graphite electrode. AmPDH/ngDH(CtCDHC310Y) anode showed not only a similar maximum voltage as with the biofuel cell based only on the ngDH(CtCDHC310Y) anode (0.55 V) but also a substantially improved maximum power output (20 Wcm(-2)) at 300 mV cell voltage in air-saturated physiological buffer. Most importantly, the estimated half-life of the mixed biofuel cell can reach up to 12 h, which is apparently longer than that of a biofuel cell in which the bioanode is based on only one single enzyme.},
  author       = {Shao, Minling and Zafar, MNadeem and Falk, Magnus and Ludwig, Roland and Sygmund, Christoph and Peterbauer, Clemens K. and Guschin, Dmitrii A. and MacAodha, Domhnall and Conghaile, Peter O. and Leech, Donal and Toscano, Miguel D. and Shleev, Sergey and Schuhmann, Wolfgang and Gorton, Lo},
  issn         = {1439-7641},
  keyword      = {biofuel cells,cellobiose dehydrogenase,coulombic efficiency,electrochemistry,electron transfer},
  language     = {eng},
  number       = {10},
  pages        = {2260--2269},
  publisher    = {John Wiley & Sons},
  series       = {ChemPhysChem},
  title        = {Optimization of a Membraneless Glucose/Oxygen Enzymatic Fuel Cell Based on a Bioanode with High Coulombic Efficiency and Current Density},
  url          = {http://dx.doi.org/10.1002/cphc.201300046},
  volume       = {14},
  year         = {2013},
}