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Subpopulations of organoid-forming cells have different motility

Jimenez, David Gomez LU ; Santos, Sofia Carreira LU ; Greiff, Lennart LU ; Alm, Kersti LU and Lindstedt, Malin LU (2020) In Applied Sciences (Switzerland) 10(13).
Abstract

Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 105 μm3. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44+ cells displayed greater motility and tumor-forming... (More)

Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 105 μm3. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44+ cells displayed greater motility and tumor-forming capacity than those of CD44- cells. These results were in line with previous reports highlighting increased invasive and tumorigenic potential in tumor cells expressing high levels of CD44. Our method provides insight into the formation of tumor organoids, and could be used to assess stemness-associated biomarkers and drug screenings on the basis of tumor organoids.

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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cancer stem cells, Digital holography, Organoid formation, Oropharyngeal squamous-cell carcinoma
in
Applied Sciences (Switzerland)
volume
10
issue
13
article number
4673
publisher
MDPI AG
external identifiers
  • scopus:85087739015
ISSN
2076-3417
DOI
10.3390/app10134673
language
English
LU publication?
yes
id
7e871da1-4b60-4991-88ef-f7fac2d5fe11
date added to LUP
2020-07-22 09:23:15
date last changed
2022-10-17 00:47:22
@article{7e871da1-4b60-4991-88ef-f7fac2d5fe11,
  abstract     = {{<p>Cancer stem cells from oropharyngeal squamous cell carcinoma (OPSCC) have the ability to self-renew and differentiate into heterogeneous three-dimensional structures carrying features of tumor cells. Here, we describe a simple and label-free method for generating tumor organoids, and imaging them using live digital holographic microscopy (DHM) on the basis of the phase shift caused by light passing through the cells. We show early events of cell aggregation during tumor-organoid formation, and display their heterogeneity in terms of optical parameters up to an optical volume of 10<sup>5</sup> μm<sup>3</sup>. Lastly, by sorting OPSCC epithelial cells, we demonstrate that CD44<sup>+</sup> cells displayed greater motility and tumor-forming capacity than those of CD44<sup>-</sup> cells. These results were in line with previous reports highlighting increased invasive and tumorigenic potential in tumor cells expressing high levels of CD44. Our method provides insight into the formation of tumor organoids, and could be used to assess stemness-associated biomarkers and drug screenings on the basis of tumor organoids.</p>}},
  author       = {{Jimenez, David Gomez and Santos, Sofia Carreira and Greiff, Lennart and Alm, Kersti and Lindstedt, Malin}},
  issn         = {{2076-3417}},
  keywords     = {{Cancer stem cells; Digital holography; Organoid formation; Oropharyngeal squamous-cell carcinoma}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{13}},
  publisher    = {{MDPI AG}},
  series       = {{Applied Sciences (Switzerland)}},
  title        = {{Subpopulations of organoid-forming cells have different motility}},
  url          = {{http://dx.doi.org/10.3390/app10134673}},
  doi          = {{10.3390/app10134673}},
  volume       = {{10}},
  year         = {{2020}},
}