Effects of ischemia on regional ligand binding to adrenoceptors in the rat brain
(1992) In Journal of the Neurological Sciences 113(2). p.165-176- Abstract
Changes in ligand binding to adrenoceptors ([3H]prazosin to α1-receptors, [3H]idazoxan to α2-receptors and [125I]cyanopindolol to β-receptors) following transient cerebral ischemia were investigated using autoradiographic methods. The binding was quantified in brain sections from control rats, rats subjected to 15 min of 2-vessel occlusion ischemia, and rats with recirculation times of 1 h, 1 week or 4 weeks after ischemia. No significant change in α1-receptor binding was observed during and immediately following ischemia, but a decrease was noted in the vulnerable hippocampal CA1 region following 1 week's survival. In the parietal cortex, the ligand binding to... (More)
Changes in ligand binding to adrenoceptors ([3H]prazosin to α1-receptors, [3H]idazoxan to α2-receptors and [125I]cyanopindolol to β-receptors) following transient cerebral ischemia were investigated using autoradiographic methods. The binding was quantified in brain sections from control rats, rats subjected to 15 min of 2-vessel occlusion ischemia, and rats with recirculation times of 1 h, 1 week or 4 weeks after ischemia. No significant change in α1-receptor binding was observed during and immediately following ischemia, but a decrease was noted in the vulnerable hippocampal CA1 region following 1 week's survival. In the parietal cortex, the ligand binding to α1-receptors increased at 4 weeks. A reduced [3H]idazoxan binding was observed 1 h after ischemia in the temporal cortex and amygdala. No change in ligand binding to β-receptors was seen in the early phase postischemia, but a marked increase had occurred in the hippocampal CA1 region at 1 and 4 weeks after ischemia (+163% and +142%, respectively), presumably due to accumulation of macrophages expressing β-receptors. The early postischemic changes in receptor binding may represent downregulation of the adrenoceptors by processes activated during ischemia, while neuronal degeneration, compensatory mechanisms in surviving neurons and proliferation of non-neuronal cells may account for the subsequent changes.
(Less)
- author
- Gustafson, Ingvar ; Westerberg, Eva J. and Wieloch, Tadeusz LU
- organization
- publishing date
- 1992-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Adrenergic receptors, Autoradiography, Cerebral ischemia, Glial proliferation, Macrophages, (Rat)
- in
- Journal of the Neurological Sciences
- volume
- 113
- issue
- 2
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0026678218
- pmid:1362586
- ISSN
- 0022-510X
- DOI
- 10.1016/0022-510X(92)90244-F
- language
- English
- LU publication?
- yes
- id
- 7e9c5cee-c779-47d7-87d8-c67058349b6d
- date added to LUP
- 2019-06-13 16:24:14
- date last changed
- 2024-01-01 10:19:42
@article{7e9c5cee-c779-47d7-87d8-c67058349b6d,
abstract = {{<p>Changes in ligand binding to adrenoceptors ([<sup>3</sup>H]prazosin to α<sub>1</sub>-receptors, [<sup>3</sup>H]idazoxan to α<sub>2</sub>-receptors and [<sup>125</sup>I]cyanopindolol to β-receptors) following transient cerebral ischemia were investigated using autoradiographic methods. The binding was quantified in brain sections from control rats, rats subjected to 15 min of 2-vessel occlusion ischemia, and rats with recirculation times of 1 h, 1 week or 4 weeks after ischemia. No significant change in α<sub>1</sub>-receptor binding was observed during and immediately following ischemia, but a decrease was noted in the vulnerable hippocampal CA1 region following 1 week's survival. In the parietal cortex, the ligand binding to α<sub>1</sub>-receptors increased at 4 weeks. A reduced [<sup>3</sup>H]idazoxan binding was observed 1 h after ischemia in the temporal cortex and amygdala. No change in ligand binding to β-receptors was seen in the early phase postischemia, but a marked increase had occurred in the hippocampal CA1 region at 1 and 4 weeks after ischemia (+163% and +142%, respectively), presumably due to accumulation of macrophages expressing β-receptors. The early postischemic changes in receptor binding may represent downregulation of the adrenoceptors by processes activated during ischemia, while neuronal degeneration, compensatory mechanisms in surviving neurons and proliferation of non-neuronal cells may account for the subsequent changes.</p>}},
author = {{Gustafson, Ingvar and Westerberg, Eva J. and Wieloch, Tadeusz}},
issn = {{0022-510X}},
keywords = {{Adrenergic receptors; Autoradiography; Cerebral ischemia; Glial proliferation; Macrophages, (Rat)}},
language = {{eng}},
month = {{01}},
number = {{2}},
pages = {{165--176}},
publisher = {{Elsevier}},
series = {{Journal of the Neurological Sciences}},
title = {{Effects of ischemia on regional ligand binding to adrenoceptors in the rat brain}},
url = {{http://dx.doi.org/10.1016/0022-510X(92)90244-F}},
doi = {{10.1016/0022-510X(92)90244-F}},
volume = {{113}},
year = {{1992}},
}