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Effects of ischemia on regional ligand binding to adrenoceptors in the rat brain

Gustafson, Ingvar ; Westerberg, Eva J. and Wieloch, Tadeusz LU (1992) In Journal of the Neurological Sciences 113(2). p.165-176
Abstract

Changes in ligand binding to adrenoceptors ([3H]prazosin to α1-receptors, [3H]idazoxan to α2-receptors and [125I]cyanopindolol to β-receptors) following transient cerebral ischemia were investigated using autoradiographic methods. The binding was quantified in brain sections from control rats, rats subjected to 15 min of 2-vessel occlusion ischemia, and rats with recirculation times of 1 h, 1 week or 4 weeks after ischemia. No significant change in α1-receptor binding was observed during and immediately following ischemia, but a decrease was noted in the vulnerable hippocampal CA1 region following 1 week's survival. In the parietal cortex, the ligand binding to... (More)

Changes in ligand binding to adrenoceptors ([3H]prazosin to α1-receptors, [3H]idazoxan to α2-receptors and [125I]cyanopindolol to β-receptors) following transient cerebral ischemia were investigated using autoradiographic methods. The binding was quantified in brain sections from control rats, rats subjected to 15 min of 2-vessel occlusion ischemia, and rats with recirculation times of 1 h, 1 week or 4 weeks after ischemia. No significant change in α1-receptor binding was observed during and immediately following ischemia, but a decrease was noted in the vulnerable hippocampal CA1 region following 1 week's survival. In the parietal cortex, the ligand binding to α1-receptors increased at 4 weeks. A reduced [3H]idazoxan binding was observed 1 h after ischemia in the temporal cortex and amygdala. No change in ligand binding to β-receptors was seen in the early phase postischemia, but a marked increase had occurred in the hippocampal CA1 region at 1 and 4 weeks after ischemia (+163% and +142%, respectively), presumably due to accumulation of macrophages expressing β-receptors. The early postischemic changes in receptor binding may represent downregulation of the adrenoceptors by processes activated during ischemia, while neuronal degeneration, compensatory mechanisms in surviving neurons and proliferation of non-neuronal cells may account for the subsequent changes.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adrenergic receptors, Autoradiography, Cerebral ischemia, Glial proliferation, Macrophages, (Rat)
in
Journal of the Neurological Sciences
volume
113
issue
2
pages
12 pages
publisher
Elsevier
external identifiers
  • scopus:0026678218
  • pmid:1362586
ISSN
0022-510X
DOI
10.1016/0022-510X(92)90244-F
language
English
LU publication?
yes
id
7e9c5cee-c779-47d7-87d8-c67058349b6d
date added to LUP
2019-06-13 16:24:14
date last changed
2021-01-03 09:00:26
@article{7e9c5cee-c779-47d7-87d8-c67058349b6d,
  abstract     = {<p>Changes in ligand binding to adrenoceptors ([<sup>3</sup>H]prazosin to α<sub>1</sub>-receptors, [<sup>3</sup>H]idazoxan to α<sub>2</sub>-receptors and [<sup>125</sup>I]cyanopindolol to β-receptors) following transient cerebral ischemia were investigated using autoradiographic methods. The binding was quantified in brain sections from control rats, rats subjected to 15 min of 2-vessel occlusion ischemia, and rats with recirculation times of 1 h, 1 week or 4 weeks after ischemia. No significant change in α<sub>1</sub>-receptor binding was observed during and immediately following ischemia, but a decrease was noted in the vulnerable hippocampal CA1 region following 1 week's survival. In the parietal cortex, the ligand binding to α<sub>1</sub>-receptors increased at 4 weeks. A reduced [<sup>3</sup>H]idazoxan binding was observed 1 h after ischemia in the temporal cortex and amygdala. No change in ligand binding to β-receptors was seen in the early phase postischemia, but a marked increase had occurred in the hippocampal CA1 region at 1 and 4 weeks after ischemia (+163% and +142%, respectively), presumably due to accumulation of macrophages expressing β-receptors. The early postischemic changes in receptor binding may represent downregulation of the adrenoceptors by processes activated during ischemia, while neuronal degeneration, compensatory mechanisms in surviving neurons and proliferation of non-neuronal cells may account for the subsequent changes.</p>},
  author       = {Gustafson, Ingvar and Westerberg, Eva J. and Wieloch, Tadeusz},
  issn         = {0022-510X},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {165--176},
  publisher    = {Elsevier},
  series       = {Journal of the Neurological Sciences},
  title        = {Effects of ischemia on regional ligand binding to adrenoceptors in the rat brain},
  url          = {http://dx.doi.org/10.1016/0022-510X(92)90244-F},
  doi          = {10.1016/0022-510X(92)90244-F},
  volume       = {113},
  year         = {1992},
}