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Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential

Lam, Matti LU ; Sanosaka, Tsukasa ; Lundin, Anders LU ; Imaizumi, Kent ; Etal, Damla ; Karlsson, Fredrik H LU ; Clausen, Maryam ; Cairns, Jonathan ; Hicks, Ryan and Kohyama, Jun , et al. (2019) In Genes to cells : devoted to molecular & cellular mechanisms 24(12). p.836-847
Abstract

We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic... (More)

We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell Line, Cell Lineage, Cells, Cultured, Human Embryonic Stem Cells/classification, Humans, Induced Pluripotent Stem Cells/classification, Neural Stem Cells/cytology, Neurogenesis, Neuroglia/cytology, Single-Cell Analysis
in
Genes to cells : devoted to molecular & cellular mechanisms
volume
24
issue
12
pages
12 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85075163983
  • pmid:31651061
ISSN
1356-9597
DOI
10.1111/gtc.12731
language
English
LU publication?
no
id
7f19688b-3f63-4c85-8047-0c3b30e7edba
date added to LUP
2021-08-09 15:31:13
date last changed
2024-04-06 06:25:57
@article{7f19688b-3f63-4c85-8047-0c3b30e7edba,
  abstract     = {{<p>We used single-cell RNA sequencing (seq) on several human induced pluripotent stem (iPS) cell-derived neural stem cell (NSC) lines and one fetal brain-derived NSC line to study inherent cell type heterogeneity at proliferating neural stem cell stage and uncovered predisposed presence of neurogenic and gliogenic progenitors. We observed heterogeneity in neurogenic progenitors that differed between the iPS cell-derived NSC lines and the fetal-derived NSC line, and we also observed differences in spontaneous differentiation potential for inhibitory and excitatory neurons between the iPS cell-derived NSC lines and the fetal-derived NSC line. In addition, using a recently published glia patterning protocol we enriched for gliogenic progenitors and generated glial cells from an iPS cell-derived NSC line.</p>}},
  author       = {{Lam, Matti and Sanosaka, Tsukasa and Lundin, Anders and Imaizumi, Kent and Etal, Damla and Karlsson, Fredrik H and Clausen, Maryam and Cairns, Jonathan and Hicks, Ryan and Kohyama, Jun and Kele, Malin and Okano, Hideyuki and Falk, Anna}},
  issn         = {{1356-9597}},
  keywords     = {{Cell Line; Cell Lineage; Cells, Cultured; Human Embryonic Stem Cells/classification; Humans; Induced Pluripotent Stem Cells/classification; Neural Stem Cells/cytology; Neurogenesis; Neuroglia/cytology; Single-Cell Analysis}},
  language     = {{eng}},
  number       = {{12}},
  pages        = {{836--847}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Genes to cells : devoted to molecular & cellular mechanisms}},
  title        = {{Single-cell study of neural stem cells derived from human iPSCs reveals distinct progenitor populations with neurogenic and gliogenic potential}},
  url          = {{https://lup.lub.lu.se/search/files/101034264/Single_cell_study_of_neural_stem.pdf}},
  doi          = {{10.1111/gtc.12731}},
  volume       = {{24}},
  year         = {{2019}},
}