Associations of human femoral condyle cartilage structure and composition with viscoelastic and constituent-specific material properties at different stages of osteoarthritis
Ebrahimi, Mohammadhossein ; Turkiewicz, Aleksandra LU ; Finnilä, Mikko A.J. ; Saarakkala, Simo ; Englund, Martin LU
; Korhonen, Rami K.
and Tanska, Petri
(2022)
In Journal of Biomechanics
145.
- Abstract
The relationships between structure and function in human knee femoral cartilage are not well-known at different stages of osteoarthritis. Thus, our aim was to characterize the depth-dependent composition and structure (proteoglycan content, collagen network organization and collagen content) of normal and osteoarthritic human femoral condyle cartilage (n = 47) and relate them to their viscoelastic and constituent-specific mechanical properties that are obtained through dynamic sinusoidal testing and fibril-reinforced poroelastic material modeling of stress-relaxation testing, respectively. We characterized the proteoglycan content using digital densitometry, collagen network organization (orientation angle and anisotropy) using... (More)
The relationships between structure and function in human knee femoral cartilage are not well-known at different stages of osteoarthritis. Thus, our aim was to characterize the depth-dependent composition and structure (proteoglycan content, collagen network organization and collagen content) of normal and osteoarthritic human femoral condyle cartilage (n = 47) and relate them to their viscoelastic and constituent-specific mechanical properties that are obtained through dynamic sinusoidal testing and fibril-reinforced poroelastic material modeling of stress-relaxation testing, respectively. We characterized the proteoglycan content using digital densitometry, collagen network organization (orientation angle and anisotropy) using polarized light microscopy and collagen content using Fourier transform infrared spectroscopy. In the superficial cartilage (0–10 % of thickness), the collagen network disorganization and proteoglycan loss were associated with the smaller initial fibril network modulus - a parameter representing the pretension of the collagen network. Furthermore, the proteoglycan loss was associated with the greater strain-dependent fibril network modulus - a measure of nonlinear mechanical behavior. The proteoglycan loss was also associated with greater cartilage viscosity at a low loading frequency (0.005 Hz), while the collagen network disorganization was associated with greater cartilage viscosity at a high loading frequency (1 Hz). Our results suggest that proteoglycan loss and collagen network disorganization reduce the pretension of the collagen network while proteoglycan degradation also increases the nonlinear mechanical behavior of the collagen network. Further, the results also highlight that proteoglycan loss and collagen disorganization increase the viscosity of femoral cartilage, but their contribution to increased viscosity occurs in completely different loading frequencies.
(Less)
- author
- Ebrahimi, Mohammadhossein
; Turkiewicz, Aleksandra
LU
; Finnilä, Mikko A.J.
; Saarakkala, Simo
; Englund, Martin
LU
; Korhonen, Rami K.
and Tanska, Petri
- organization
- publishing date
- 2022-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Biomechanics, Collagen fibril network, Fibril-reinforced poroelastic, Finite element modeling, Proteoglycan
- in
- Journal of Biomechanics
- volume
- 145
- article number
- 111390
- publisher
- Elsevier
- external identifiers
-
- pmid:36442429
- scopus:85142763340
- ISSN
- 0021-9290
- DOI
- 10.1016/j.jbiomech.2022.111390
- language
- English
- LU publication?
- yes
- id
- 7f1a771b-bf5f-456d-917c-30e8b775269a
- date added to LUP
- 2022-12-20 15:26:12
- date last changed
- 2024-04-18 16:48:50
@article{7f1a771b-bf5f-456d-917c-30e8b775269a,
abstract = {{<p>The relationships between structure and function in human knee femoral cartilage are not well-known at different stages of osteoarthritis. Thus, our aim was to characterize the depth-dependent composition and structure (proteoglycan content, collagen network organization and collagen content) of normal and osteoarthritic human femoral condyle cartilage (n = 47) and relate them to their viscoelastic and constituent-specific mechanical properties that are obtained through dynamic sinusoidal testing and fibril-reinforced poroelastic material modeling of stress-relaxation testing, respectively. We characterized the proteoglycan content using digital densitometry, collagen network organization (orientation angle and anisotropy) using polarized light microscopy and collagen content using Fourier transform infrared spectroscopy. In the superficial cartilage (0–10 % of thickness), the collagen network disorganization and proteoglycan loss were associated with the smaller initial fibril network modulus - a parameter representing the pretension of the collagen network. Furthermore, the proteoglycan loss was associated with the greater strain-dependent fibril network modulus - a measure of nonlinear mechanical behavior. The proteoglycan loss was also associated with greater cartilage viscosity at a low loading frequency (0.005 Hz), while the collagen network disorganization was associated with greater cartilage viscosity at a high loading frequency (1 Hz). Our results suggest that proteoglycan loss and collagen network disorganization reduce the pretension of the collagen network while proteoglycan degradation also increases the nonlinear mechanical behavior of the collagen network. Further, the results also highlight that proteoglycan loss and collagen disorganization increase the viscosity of femoral cartilage, but their contribution to increased viscosity occurs in completely different loading frequencies.</p>}},
author = {{Ebrahimi, Mohammadhossein and Turkiewicz, Aleksandra and Finnilä, Mikko A.J. and Saarakkala, Simo and Englund, Martin and Korhonen, Rami K. and Tanska, Petri}},
issn = {{0021-9290}},
keywords = {{Biomechanics; Collagen fibril network; Fibril-reinforced poroelastic; Finite element modeling; Proteoglycan}},
language = {{eng}},
publisher = {{Elsevier}},
series = {{Journal of Biomechanics}},
title = {{Associations of human femoral condyle cartilage structure and composition with viscoelastic and constituent-specific material properties at different stages of osteoarthritis}},
url = {{http://dx.doi.org/10.1016/j.jbiomech.2022.111390}},
doi = {{10.1016/j.jbiomech.2022.111390}},
volume = {{145}},
year = {{2022}},
}