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Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis

Song, Chenchen ; Sun, Xuan LU ; Wang, Yao ; Bülow, Leif LU ; Mecklenburg, Michael ; Wu, Changxin ; Meng, Qinglai and Xie, Bin LU (2023) In Frontiers in cellular and infection microbiology 13.
Abstract
Antibiotic resistance has become a serious threat to global public health and economic development. Rapid and accurate identification of a patient status for antimicrobial resistance (AMR) are urgently needed in clinical diagnosis. Here we describe the development of an assay method for activity fingerprinting of AMR β-lactamases using panels of 7 β-lactam antibiotics in 35 min. New Deli Metallo β-lactamase-1 (NDM-1) and penicillinase were demonstrated as two different classes of β-lactamases. The panel consisted of three classes of antibiotics, including: penicillins (penicillin G, piperacillin), cephalosporins (cefepime, ceftriaxone, cefazolin) and carbapenems (meropenem and imipenem). The assay employed a scheme combines the catalytic... (More)
Antibiotic resistance has become a serious threat to global public health and economic development. Rapid and accurate identification of a patient status for antimicrobial resistance (AMR) are urgently needed in clinical diagnosis. Here we describe the development of an assay method for activity fingerprinting of AMR β-lactamases using panels of 7 β-lactam antibiotics in 35 min. New Deli Metallo β-lactamase-1 (NDM-1) and penicillinase were demonstrated as two different classes of β-lactamases. The panel consisted of three classes of antibiotics, including: penicillins (penicillin G, piperacillin), cephalosporins (cefepime, ceftriaxone, cefazolin) and carbapenems (meropenem and imipenem). The assay employed a scheme combines the catalytic reaction of AMR β-lactamases on antibiotic substrates with a flow-injected thermometric biosensor that allows the direct detection of the heat generated from the enzymatic catalysis, and eliminates the need for custom substrates and multiple detection schemes. In order to differentiate classes of β-lactamases, characterization of the enzyme activity under different catalytic condition, such as, buffer composition, ion strength and pH were investigated. This assay could provide a tool for fast diagnosis of patient AMR status which makes possible for the future accurate treatment with selected antibiotics. (Less)
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type
Contribution to journal
publication status
published
subject
in
Frontiers in cellular and infection microbiology
volume
13
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85171556867
  • pmid:37743856
ISSN
2235-2988
DOI
10.3389/fcimb.2023.1222156
language
English
LU publication?
yes
id
7f39fad3-1fef-4960-ae7c-61094f7c56dd
date added to LUP
2023-09-12 09:49:48
date last changed
2023-12-14 03:00:44
@article{7f39fad3-1fef-4960-ae7c-61094f7c56dd,
  abstract     = {{Antibiotic resistance has become a serious threat to global public health and economic development. Rapid and accurate identification of a patient status for antimicrobial resistance (AMR) are urgently needed in clinical diagnosis. Here we describe the development of an assay method for activity fingerprinting of AMR β-lactamases using panels of 7 β-lactam antibiotics in 35 min. New Deli Metallo β-lactamase-1 (NDM-1) and penicillinase were demonstrated as two different classes of β-lactamases. The panel consisted of three classes of antibiotics, including: penicillins (penicillin G, piperacillin), cephalosporins (cefepime, ceftriaxone, cefazolin) and carbapenems (meropenem and imipenem). The assay employed a scheme combines the catalytic reaction of AMR β-lactamases on antibiotic substrates with a flow-injected thermometric biosensor that allows the direct detection of the heat generated from the enzymatic catalysis, and eliminates the need for custom substrates and multiple detection schemes. In order to differentiate classes of β-lactamases, characterization of the enzyme activity under different catalytic condition, such as, buffer composition, ion strength and pH were investigated. This assay could provide a tool for fast diagnosis of patient AMR status which makes possible for the future accurate treatment with selected antibiotics.}},
  author       = {{Song, Chenchen and Sun, Xuan and Wang, Yao and Bülow, Leif and Mecklenburg, Michael and Wu, Changxin and Meng, Qinglai and Xie, Bin}},
  issn         = {{2235-2988}},
  language     = {{eng}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in cellular and infection microbiology}},
  title        = {{Activity fingerprinting of AMR β-lactamase towards a fast and accurate diagnosis}},
  url          = {{http://dx.doi.org/10.3389/fcimb.2023.1222156}},
  doi          = {{10.3389/fcimb.2023.1222156}},
  volume       = {{13}},
  year         = {{2023}},
}