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Ultrasound-Guided Intrauterine Labeling of Rat Fetuses

Gumus, Hatice Gulcin; Illa, Miriam; Pla, Laura; Gonzalez-Neira, Anna; Demicheva, Elena; Ley, David LU ; Crispi, Fatima and Gratacos, Eduard (2017) In Gynecologic and Obstetric Investigation
Abstract

Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates... (More)

Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates from the intra-amniotic injections group were labeled at birth, while 93% of the neonates from fetal subcutaneous injections group were tagged, showing a visible spot in the skin at birth. Conclusion: Our results suggest that ultrasound-guided fetal subcutaneous injections might be an adequate strategy for selectively labeling fetuses in multifetal pregnant animals.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animal model, Fetal subcutaneous tagging, Intra-amniotic injections, Pregnancy, Ultrasound-guided injections, Vital dyes
in
Gynecologic and Obstetric Investigation
publisher
Karger
external identifiers
  • scopus:85025434913
ISSN
0378-7346
DOI
10.1159/000454766
language
English
LU publication?
yes
id
7f46d991-ddce-4c94-abec-4c3389f9d8dd
date added to LUP
2017-08-29 10:15:10
date last changed
2018-01-07 12:16:19
@article{7f46d991-ddce-4c94-abec-4c3389f9d8dd,
  abstract     = {<p>Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates from the intra-amniotic injections group were labeled at birth, while 93% of the neonates from fetal subcutaneous injections group were tagged, showing a visible spot in the skin at birth. Conclusion: Our results suggest that ultrasound-guided fetal subcutaneous injections might be an adequate strategy for selectively labeling fetuses in multifetal pregnant animals.</p>},
  author       = {Gumus, Hatice Gulcin and Illa, Miriam and Pla, Laura and Gonzalez-Neira, Anna and Demicheva, Elena and Ley, David and Crispi, Fatima and Gratacos, Eduard},
  issn         = {0378-7346},
  keyword      = {Animal model,Fetal subcutaneous tagging,Intra-amniotic injections,Pregnancy,Ultrasound-guided injections,Vital dyes},
  language     = {eng},
  month        = {07},
  publisher    = {Karger},
  series       = {Gynecologic and Obstetric Investigation},
  title        = {Ultrasound-Guided Intrauterine Labeling of Rat Fetuses},
  url          = {http://dx.doi.org/10.1159/000454766},
  year         = {2017},
}