Ultrasound-Guided Intrauterine Labeling of Rat Fetuses
(2017) In Gynecologic and Obstetric Investigation- Abstract
Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates... (More)
Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates from the intra-amniotic injections group were labeled at birth, while 93% of the neonates from fetal subcutaneous injections group were tagged, showing a visible spot in the skin at birth. Conclusion: Our results suggest that ultrasound-guided fetal subcutaneous injections might be an adequate strategy for selectively labeling fetuses in multifetal pregnant animals.
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- author
- Gumus, Hatice Gulcin ; Illa, Miriam ; Pla, Laura ; Gonzalez-Neira, Anna ; Demicheva, Elena ; Ley, David LU ; Crispi, Fatima and Gratacos, Eduard
- organization
- publishing date
- 2017-07-18
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animal model, Fetal subcutaneous tagging, Intra-amniotic injections, Pregnancy, Ultrasound-guided injections, Vital dyes
- in
- Gynecologic and Obstetric Investigation
- publisher
- Karger
- external identifiers
-
- pmid:28719908
- scopus:85025434913
- ISSN
- 0378-7346
- DOI
- 10.1159/000454766
- language
- English
- LU publication?
- yes
- id
- 7f46d991-ddce-4c94-abec-4c3389f9d8dd
- date added to LUP
- 2017-08-29 10:15:10
- date last changed
- 2024-03-17 19:41:14
@article{7f46d991-ddce-4c94-abec-4c3389f9d8dd,
abstract = {{<p>Aim: To compare intra-amniotic versus fetal subcutaneous injections for selective fetal labeling in multifetal rat pregnancies. Methods: A total of 14 pregnant rats were randomized to receive intra-amniotic injections of dyes (including Fluorescein, Indigo Carmine, or Evans Blue) or fetal subcutaneous injections (of commercial tattoo ink) both guided by ultrasound at 15-17 days of gestation. Survival, injection, and labeling success rates of both techniques were compared. Results: Survival rates (84.4% for intra-amniotic injections vs. 90.9% for fetal subcutaneous injections) and injection success rates (94% for intra-amniotic injections vs. 100% for fetal subcutaneous injections) were similar among both groups. None of the neonates from the intra-amniotic injections group were labeled at birth, while 93% of the neonates from fetal subcutaneous injections group were tagged, showing a visible spot in the skin at birth. Conclusion: Our results suggest that ultrasound-guided fetal subcutaneous injections might be an adequate strategy for selectively labeling fetuses in multifetal pregnant animals.</p>}},
author = {{Gumus, Hatice Gulcin and Illa, Miriam and Pla, Laura and Gonzalez-Neira, Anna and Demicheva, Elena and Ley, David and Crispi, Fatima and Gratacos, Eduard}},
issn = {{0378-7346}},
keywords = {{Animal model; Fetal subcutaneous tagging; Intra-amniotic injections; Pregnancy; Ultrasound-guided injections; Vital dyes}},
language = {{eng}},
month = {{07}},
publisher = {{Karger}},
series = {{Gynecologic and Obstetric Investigation}},
title = {{Ultrasound-Guided Intrauterine Labeling of Rat Fetuses}},
url = {{http://dx.doi.org/10.1159/000454766}},
doi = {{10.1159/000454766}},
year = {{2017}},
}