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Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells

Grassi, Daniela A. LU ; Brattås, Per Ludvik LU ; Jönsson, Marie E. LU ; Atacho, Diahann LU orcid ; Karlsson, Ofelia ; Nolbrant, Sara LU ; Parmar, Malin LU orcid and Jakobsson, Johan LU orcid (2020) In Heliyon 6(1).
Abstract

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be differentiated into many different cell types of the central nervous system. One challenge when using pluripotent stem cells is to develop robust and efficient differentiation protocols that result in homogenous cultures of the desired cell type. Here, we have utilized the SMAD-inhibitors SB431542 and Noggin in a fully defined monolayer culture model to differentiate human pluripotent cells into homogenous forebrain neural progenitors. Temporal fate analysis revealed that this protocol results in forebrain-patterned neural progenitor cells that start to express early neuronal markers after two weeks of differentiation, allowing for the analysis of gene... (More)

Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be differentiated into many different cell types of the central nervous system. One challenge when using pluripotent stem cells is to develop robust and efficient differentiation protocols that result in homogenous cultures of the desired cell type. Here, we have utilized the SMAD-inhibitors SB431542 and Noggin in a fully defined monolayer culture model to differentiate human pluripotent cells into homogenous forebrain neural progenitors. Temporal fate analysis revealed that this protocol results in forebrain-patterned neural progenitor cells that start to express early neuronal markers after two weeks of differentiation, allowing for the analysis of gene expression changes during neurogenesis. Using this system, we were able to identify many previously uncharacterized long intergenic non-coding RNAs that display dynamic expression during human forebrain neurogenesis. Cell biology; Genetics; Neuroscience; Developmental genetics; Cellular neuroscience; lincRNAs; Forebrain development; Induced pluripotent stem cells; Neural progenitor cells; Differentiation

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author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cell biology, Cellular neuroscience, Developmental genetics, Differentiation, Forebrain development, Genetics, Induced pluripotent stem cells, lincRNAs, Neural progenitor cells, Neuroscience
in
Heliyon
volume
6
issue
1
article number
e03067
publisher
Elsevier
external identifiers
  • pmid:31909251
  • scopus:85077151143
ISSN
2405-8440
DOI
10.1016/j.heliyon.2019.e03067
language
English
LU publication?
yes
id
7f605452-998b-4e8e-a1d7-9cc9ae6fb2bf
date added to LUP
2020-01-10 11:14:20
date last changed
2024-10-02 19:19:25
@article{7f605452-998b-4e8e-a1d7-9cc9ae6fb2bf,
  abstract     = {{<p>Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be differentiated into many different cell types of the central nervous system. One challenge when using pluripotent stem cells is to develop robust and efficient differentiation protocols that result in homogenous cultures of the desired cell type. Here, we have utilized the SMAD-inhibitors SB431542 and Noggin in a fully defined monolayer culture model to differentiate human pluripotent cells into homogenous forebrain neural progenitors. Temporal fate analysis revealed that this protocol results in forebrain-patterned neural progenitor cells that start to express early neuronal markers after two weeks of differentiation, allowing for the analysis of gene expression changes during neurogenesis. Using this system, we were able to identify many previously uncharacterized long intergenic non-coding RNAs that display dynamic expression during human forebrain neurogenesis. Cell biology; Genetics; Neuroscience; Developmental genetics; Cellular neuroscience; lincRNAs; Forebrain development; Induced pluripotent stem cells; Neural progenitor cells; Differentiation</p>}},
  author       = {{Grassi, Daniela A. and Brattås, Per Ludvik and Jönsson, Marie E. and Atacho, Diahann and Karlsson, Ofelia and Nolbrant, Sara and Parmar, Malin and Jakobsson, Johan}},
  issn         = {{2405-8440}},
  keywords     = {{Cell biology; Cellular neuroscience; Developmental genetics; Differentiation; Forebrain development; Genetics; Induced pluripotent stem cells; lincRNAs; Neural progenitor cells; Neuroscience}},
  language     = {{eng}},
  number       = {{1}},
  publisher    = {{Elsevier}},
  series       = {{Heliyon}},
  title        = {{Profiling of lincRNAs in human pluripotent stem cell derived forebrain neural progenitor cells}},
  url          = {{http://dx.doi.org/10.1016/j.heliyon.2019.e03067}},
  doi          = {{10.1016/j.heliyon.2019.e03067}},
  volume       = {{6}},
  year         = {{2020}},
}