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Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster

Gustafsson, Robert ; Berntsson, Ronnie P-A ; Martínez-Carranza, Markel ; El Tekle, Geniver ; Odegrip, Richard ; Johnson, Eric A and Stenmark, Pål LU orcid (2017) In FEBS Letters 591(22). p.3781-3792
Abstract

Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.

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author
; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
Bacterial Proteins/chemistry, Cloning, Molecular, Clostridium botulinum/chemistry, Crystallography, X-Ray, Models, Molecular, Multigene Family, Phosphatidylinositols/metabolism, Protein Binding, Protein Conformation
in
FEBS Letters
volume
591
issue
22
pages
3781 - 3792
publisher
Wiley-Blackwell
external identifiers
  • scopus:85032900490
  • pmid:29067689
ISSN
1873-3468
DOI
10.1002/1873-3468.12889
language
English
LU publication?
no
additional info
© 2017 Federation of European Biochemical Societies.
id
7f65a6a9-b6f7-47a9-b78c-d0f6dbbeb99d
date added to LUP
2019-04-30 07:53:18
date last changed
2024-04-30 05:25:54
@article{7f65a6a9-b6f7-47a9-b78c-d0f6dbbeb99d,
  abstract     = {{<p>Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.</p>}},
  author       = {{Gustafsson, Robert and Berntsson, Ronnie P-A and Martínez-Carranza, Markel and El Tekle, Geniver and Odegrip, Richard and Johnson, Eric A and Stenmark, Pål}},
  issn         = {{1873-3468}},
  keywords     = {{Bacterial Proteins/chemistry; Cloning, Molecular; Clostridium botulinum/chemistry; Crystallography, X-Ray; Models, Molecular; Multigene Family; Phosphatidylinositols/metabolism; Protein Binding; Protein Conformation}},
  language     = {{eng}},
  number       = {{22}},
  pages        = {{3781--3792}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster}},
  url          = {{http://dx.doi.org/10.1002/1873-3468.12889}},
  doi          = {{10.1002/1873-3468.12889}},
  volume       = {{591}},
  year         = {{2017}},
}