Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster
(2017) In FEBS Letters 591(22). p.3781-3792- Abstract
Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.
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https://lup.lub.lu.se/record/7f65a6a9-b6f7-47a9-b78c-d0f6dbbeb99d
- author
- Gustafsson, Robert ; Berntsson, Ronnie P-A ; Martínez-Carranza, Markel ; El Tekle, Geniver ; Odegrip, Richard ; Johnson, Eric A and Stenmark, Pål LU
- publishing date
- 2017-11
- type
- Contribution to journal
- publication status
- published
- keywords
- Bacterial Proteins/chemistry, Cloning, Molecular, Clostridium botulinum/chemistry, Crystallography, X-Ray, Models, Molecular, Multigene Family, Phosphatidylinositols/metabolism, Protein Binding, Protein Conformation
- in
- FEBS Letters
- volume
- 591
- issue
- 22
- pages
- 3781 - 3792
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:29067689
- scopus:85032900490
- ISSN
- 1873-3468
- DOI
- 10.1002/1873-3468.12889
- language
- English
- LU publication?
- no
- additional info
- © 2017 Federation of European Biochemical Societies.
- id
- 7f65a6a9-b6f7-47a9-b78c-d0f6dbbeb99d
- date added to LUP
- 2019-04-30 07:53:18
- date last changed
- 2024-08-20 14:51:24
@article{7f65a6a9-b6f7-47a9-b78c-d0f6dbbeb99d, abstract = {{<p>Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.</p>}}, author = {{Gustafsson, Robert and Berntsson, Ronnie P-A and Martínez-Carranza, Markel and El Tekle, Geniver and Odegrip, Richard and Johnson, Eric A and Stenmark, Pål}}, issn = {{1873-3468}}, keywords = {{Bacterial Proteins/chemistry; Cloning, Molecular; Clostridium botulinum/chemistry; Crystallography, X-Ray; Models, Molecular; Multigene Family; Phosphatidylinositols/metabolism; Protein Binding; Protein Conformation}}, language = {{eng}}, number = {{22}}, pages = {{3781--3792}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Letters}}, title = {{Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster}}, url = {{http://dx.doi.org/10.1002/1873-3468.12889}}, doi = {{10.1002/1873-3468.12889}}, volume = {{591}}, year = {{2017}}, }