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CDK9 Regulates Apoptosis of Myoblast Cells by Modulation of microRNA-1 Expression

Tarhriz, Vahideh ; Wagner, Kay Dietrich ; Masoumi, Zahra LU orcid ; Molavi, Ommoleila ; Hejazi, Mohammad Saeid and Ghanbarian, Hossein (2018) In Journal of Cellular Biochemistry 119(1). p.547-554
Abstract

Cdk9 is the catalytic core of the positive transcription elongation factor b (P-TEFb) and regulates transcriptional elongation factors by phosphorylation of RNA pol II. Apart from its role on myogenic gene expression, Cdk9 regulation of muscle-specific microRNAs in the early stage of cardiomyogenesis is poorly understood. Here we demonstrate that Cdk9 not only regulates myogenic transcription factors, but also controls muscle-specific microRNAs. During cardiac differentiation of mouse embryonic stem cells, high Cdk9 expression preceded up-regulation of miR-1. To investigate potential regulatory roles of Cdk9 on cardiac microRNAs and myogenesis genes, we overexpressed Cdk9 in myoblast C2C12 cells, which resulted in significant induction... (More)

Cdk9 is the catalytic core of the positive transcription elongation factor b (P-TEFb) and regulates transcriptional elongation factors by phosphorylation of RNA pol II. Apart from its role on myogenic gene expression, Cdk9 regulation of muscle-specific microRNAs in the early stage of cardiomyogenesis is poorly understood. Here we demonstrate that Cdk9 not only regulates myogenic transcription factors, but also controls muscle-specific microRNAs. During cardiac differentiation of mouse embryonic stem cells, high Cdk9 expression preceded up-regulation of miR-1. To investigate potential regulatory roles of Cdk9 on cardiac microRNAs and myogenesis genes, we overexpressed Cdk9 in myoblast C2C12 cells, which resulted in significant induction of miR-1 and miR-206, while miR-133 was downregulated. Moreover, expression levels of MyoD and Srf, key regulators of myogenesis, also increased in cells with overexpression of Cdk9. We further observed Cdk9-mediated apoptosis in C2C12 cells corresponding to induction of miR-1 expression levels. Thus, Cdk9 plays a complex role in myocyte progenitor differentiation and apoptosis by regulating myogenic protein and muscle-specific microRNA expression. J. Cell. Biochem. 119: 547–554, 2018.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
APOPTOSIS, CDK9, IN VITRO DIFFERENTIATION, microRNA, MYOCYTE PROGENITOR, PROLIFERATION
in
Journal of Cellular Biochemistry
volume
119
issue
1
pages
8 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85022333521
  • pmid:28608935
ISSN
0730-2312
DOI
10.1002/jcb.26213
language
English
LU publication?
yes
id
7f7abb74-022e-4b75-ac5c-68ea550521a4
date added to LUP
2020-04-22 14:27:47
date last changed
2024-07-10 15:06:05
@article{7f7abb74-022e-4b75-ac5c-68ea550521a4,
  abstract     = {{<p>Cdk9 is the catalytic core of the positive transcription elongation factor b (P-TEFb) and regulates transcriptional elongation factors by phosphorylation of RNA pol II. Apart from its role on myogenic gene expression, Cdk9 regulation of muscle-specific microRNAs in the early stage of cardiomyogenesis is poorly understood. Here we demonstrate that Cdk9 not only regulates myogenic transcription factors, but also controls muscle-specific microRNAs. During cardiac differentiation of mouse embryonic stem cells, high Cdk9 expression preceded up-regulation of miR-1. To investigate potential regulatory roles of Cdk9 on cardiac microRNAs and myogenesis genes, we overexpressed Cdk9 in myoblast C2C12 cells, which resulted in significant induction of miR-1 and miR-206, while miR-133 was downregulated. Moreover, expression levels of MyoD and Srf, key regulators of myogenesis, also increased in cells with overexpression of Cdk9. We further observed Cdk9-mediated apoptosis in C2C12 cells corresponding to induction of miR-1 expression levels. Thus, Cdk9 plays a complex role in myocyte progenitor differentiation and apoptosis by regulating myogenic protein and muscle-specific microRNA expression. J. Cell. Biochem. 119: 547–554, 2018.</p>}},
  author       = {{Tarhriz, Vahideh and Wagner, Kay Dietrich and Masoumi, Zahra and Molavi, Ommoleila and Hejazi, Mohammad Saeid and Ghanbarian, Hossein}},
  issn         = {{0730-2312}},
  keywords     = {{APOPTOSIS; CDK9; IN VITRO DIFFERENTIATION; microRNA; MYOCYTE PROGENITOR; PROLIFERATION}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{547--554}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Journal of Cellular Biochemistry}},
  title        = {{CDK9 Regulates Apoptosis of Myoblast Cells by Modulation of microRNA-1 Expression}},
  url          = {{http://dx.doi.org/10.1002/jcb.26213}},
  doi          = {{10.1002/jcb.26213}},
  volume       = {{119}},
  year         = {{2018}},
}