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Long-term full-thickness embryonic rabbit retinal transplants

Ghosh, F LU ; Johansson, Kjell LU and Ehinger, B LU (1999) In Investigative Ophthalmology and Visual Science 40(1). p.42-133
Abstract

PURPOSE: To establish the light and electron microscopic morphology of long-term full-thickness embryonic rabbit retinal transplants, with special attention paid to graft- host integration.

METHODS: Eighteen rabbits received a complete embryonic neuroretina 19 days after conception. The transplants were positioned under the host retina, flat against the host retinal pigment epithelium with proper polarity, using a vitrectomy technique. After surviving 3 to 10 months, the transplants were examined by light and electron microscopy.

RESULTS: The outer retina of the host had degenerated in all specimens. In 16 of the 18 eyes, well-laminated transplants with correct polarity, measuring up to 3.2 mm in length, were found. The... (More)

PURPOSE: To establish the light and electron microscopic morphology of long-term full-thickness embryonic rabbit retinal transplants, with special attention paid to graft- host integration.

METHODS: Eighteen rabbits received a complete embryonic neuroretina 19 days after conception. The transplants were positioned under the host retina, flat against the host retinal pigment epithelium with proper polarity, using a vitrectomy technique. After surviving 3 to 10 months, the transplants were examined by light and electron microscopy.

RESULTS: The outer retina of the host had degenerated in all specimens. In 16 of the 18 eyes, well-laminated transplants with correct polarity, measuring up to 3.2 mm in length, were found. The transplants displayed long outer segments facing the host retinal pigment epithelium, and they were laminated to the level of the inner plexiform layer in which fusion with the host was often evident. Fusion was more prominent in the oldest transplants. Electron microscopy revealed bundles of neurites at different levels of maturation in close contact with Müller cell fimbriae at regular intervals along the graft-host border.

CONCLUSIONS: Full-thickness embryonic rabbit retinal transplants positioned with correct polarity develop into large laminated retinas and survive without immunosuppression for at least 10 months. Host and graft adapt and almost reconstruct the normal retinal appearance. Ultrastructurally, well-developed photoreceptors and many normal synapse types are seen, and neuron sprouting is evident at the graft-host border.

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organization
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Contribution to journal
publication status
published
subject
keywords
Animals, Cell Polarity, Fetal Tissue Transplantation, Neural Pathways, Neurons, Rabbits, Retina, Retinal Cone Photoreceptor Cells, Retinal Ganglion Cells, Retinal Rod Photoreceptor Cells, Journal Article, Research Support, Non-U.S. Gov't
in
Investigative Ophthalmology and Visual Science
volume
40
issue
1
pages
10 pages
publisher
ASSOC RESEARCH VISION OPHTHALMOLOGY INC
external identifiers
  • scopus:0032952546
ISSN
0146-0404
language
English
LU publication?
yes
id
7f94f080-7ebe-4956-a894-5282d55bcbbd
alternative location
http://iovs.arvojournals.org/article.aspx?articleid=2162040
date added to LUP
2017-05-17 11:39:19
date last changed
2017-08-27 06:41:05
@article{7f94f080-7ebe-4956-a894-5282d55bcbbd,
  abstract     = {<p>PURPOSE: To establish the light and electron microscopic morphology of long-term full-thickness embryonic rabbit retinal transplants, with special attention paid to graft- host integration.</p><p>METHODS: Eighteen rabbits received a complete embryonic neuroretina 19 days after conception. The transplants were positioned under the host retina, flat against the host retinal pigment epithelium with proper polarity, using a vitrectomy technique. After surviving 3 to 10 months, the transplants were examined by light and electron microscopy.</p><p>RESULTS: The outer retina of the host had degenerated in all specimens. In 16 of the 18 eyes, well-laminated transplants with correct polarity, measuring up to 3.2 mm in length, were found. The transplants displayed long outer segments facing the host retinal pigment epithelium, and they were laminated to the level of the inner plexiform layer in which fusion with the host was often evident. Fusion was more prominent in the oldest transplants. Electron microscopy revealed bundles of neurites at different levels of maturation in close contact with Müller cell fimbriae at regular intervals along the graft-host border.</p><p>CONCLUSIONS: Full-thickness embryonic rabbit retinal transplants positioned with correct polarity develop into large laminated retinas and survive without immunosuppression for at least 10 months. Host and graft adapt and almost reconstruct the normal retinal appearance. Ultrastructurally, well-developed photoreceptors and many normal synapse types are seen, and neuron sprouting is evident at the graft-host border.</p>},
  author       = {Ghosh, F and Johansson, Kjell and Ehinger, B},
  issn         = {0146-0404},
  keyword      = {Animals,Cell Polarity,Fetal Tissue Transplantation,Neural Pathways,Neurons,Rabbits,Retina,Retinal Cone Photoreceptor Cells,Retinal Ganglion Cells,Retinal Rod Photoreceptor Cells,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {42--133},
  publisher    = {ASSOC RESEARCH VISION OPHTHALMOLOGY INC},
  series       = {Investigative Ophthalmology and Visual Science},
  title        = {Long-term full-thickness embryonic rabbit retinal transplants},
  volume       = {40},
  year         = {1999},
}