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1,2 Propanediol utilization by Lactobacillus reuteri DSM 20016, role in bioconversion of glycerol to 1,3 propanediol, 3-hydroxypropionaldehyde and 3-hydroxypropionic acid

Amin, Heba M. ; Hashem, Abdelgawad M. ; Ashour, Mohamed S. and Hatti-Kaul, Rajini LU (2013) In Journal of Genetic Engineering and Biotechnology 11(1). p.53-59
Abstract

The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose.The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72... (More)

The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose.The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72 h and the metabolites produced during the process were detected using HPLC. 1,2 Propanediol was completely converted to propionaldhyde in a time dependent fashion, this process had a higher rate in presence of glucose. Consequently the produced propionaldhyde was converted to propionic acid and propanol in a skewed equimolar manner. In presence of glucose: acetic acid, lactic acid, succinic acid and ethanol were detected while in absence of glucose only minute amounts of acetic acid and lactic acid were detected which indicates presence of different metabolic pathways for glucose and 1,2 propanediol metabolism. Resting cells of L. reuteri induced in presence of 1,2 propanediol have shown significant capabilities to convert aqueous glycerol to 1,3 propanediol, 3-hydroxypropionaldhyde and a compound proposed to be 3-hydroxypropionic acid as detected by gas chromatographic technique.

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author
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Contribution to journal
publication status
published
subject
keywords
1,2 Propanediol (1,2 PD), 1,3 Propanediol (1,3 PD), 3-Hydroxypropionaldehyde (3-HPA), Lactobacillus reuteri, Propionaldhyde, Propionic acid
in
Journal of Genetic Engineering and Biotechnology
volume
11
issue
1
pages
7 pages
publisher
Academy of Scientific Research and Technology
external identifiers
  • scopus:84969834711
ISSN
1687-157X
DOI
10.1016/j.jgeb.2012.12.002
language
English
LU publication?
yes
id
7fa95bad-0a1c-45c8-b4f9-32f5db20fafc
date added to LUP
2020-05-09 11:06:30
date last changed
2020-09-16 04:45:10
@article{7fa95bad-0a1c-45c8-b4f9-32f5db20fafc,
  abstract     = {<p>The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose.The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72 h and the metabolites produced during the process were detected using HPLC. 1,2 Propanediol was completely converted to propionaldhyde in a time dependent fashion, this process had a higher rate in presence of glucose. Consequently the produced propionaldhyde was converted to propionic acid and propanol in a skewed equimolar manner. In presence of glucose: acetic acid, lactic acid, succinic acid and ethanol were detected while in absence of glucose only minute amounts of acetic acid and lactic acid were detected which indicates presence of different metabolic pathways for glucose and 1,2 propanediol metabolism. Resting cells of L. reuteri induced in presence of 1,2 propanediol have shown significant capabilities to convert aqueous glycerol to 1,3 propanediol, 3-hydroxypropionaldhyde and a compound proposed to be 3-hydroxypropionic acid as detected by gas chromatographic technique.</p>},
  author       = {Amin, Heba M. and Hashem, Abdelgawad M. and Ashour, Mohamed S. and Hatti-Kaul, Rajini},
  issn         = {1687-157X},
  language     = {eng},
  month        = {06},
  number       = {1},
  pages        = {53--59},
  publisher    = {Academy of Scientific Research and Technology},
  series       = {Journal of Genetic Engineering and Biotechnology},
  title        = {1,2 Propanediol utilization by Lactobacillus reuteri DSM 20016, role in bioconversion of glycerol to 1,3 propanediol, 3-hydroxypropionaldehyde and 3-hydroxypropionic acid},
  url          = {http://dx.doi.org/10.1016/j.jgeb.2012.12.002},
  doi          = {10.1016/j.jgeb.2012.12.002},
  volume       = {11},
  year         = {2013},
}