1,2 Propanediol utilization by Lactobacillus reuteri DSM 20016, role in bioconversion of glycerol to 1,3 propanediol, 3-hydroxypropionaldehyde and 3-hydroxypropionic acid
(2013) In Journal of Genetic Engineering and Biotechnology 11(1). p.53-59- Abstract
The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose.The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72... (More)
The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose.The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72 h and the metabolites produced during the process were detected using HPLC. 1,2 Propanediol was completely converted to propionaldhyde in a time dependent fashion, this process had a higher rate in presence of glucose. Consequently the produced propionaldhyde was converted to propionic acid and propanol in a skewed equimolar manner. In presence of glucose: acetic acid, lactic acid, succinic acid and ethanol were detected while in absence of glucose only minute amounts of acetic acid and lactic acid were detected which indicates presence of different metabolic pathways for glucose and 1,2 propanediol metabolism. Resting cells of L. reuteri induced in presence of 1,2 propanediol have shown significant capabilities to convert aqueous glycerol to 1,3 propanediol, 3-hydroxypropionaldhyde and a compound proposed to be 3-hydroxypropionic acid as detected by gas chromatographic technique.
(Less)
- author
- Amin, Heba M. ; Hashem, Abdelgawad M. ; Ashour, Mohamed S. and Hatti-Kaul, Rajini LU
- organization
- publishing date
- 2013-06-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 1,2 Propanediol (1,2 PD), 1,3 Propanediol (1,3 PD), 3-Hydroxypropionaldehyde (3-HPA), Lactobacillus reuteri, Propionaldhyde, Propionic acid
- in
- Journal of Genetic Engineering and Biotechnology
- volume
- 11
- issue
- 1
- pages
- 7 pages
- publisher
- Academy of Scientific Research and Technology
- external identifiers
-
- scopus:84969834711
- ISSN
- 1687-157X
- DOI
- 10.1016/j.jgeb.2012.12.002
- language
- English
- LU publication?
- yes
- id
- 7fa95bad-0a1c-45c8-b4f9-32f5db20fafc
- date added to LUP
- 2020-05-09 11:06:30
- date last changed
- 2022-04-03 02:19:32
@article{7fa95bad-0a1c-45c8-b4f9-32f5db20fafc,
abstract = {{<p>The objective of the presented work is to demonstrate the metabolism of 1,2 propandiol by Lactobacillus reuteri and to elucidate the metabolites produced during the process. This Metabolic pathway is crucial for biotechnological applications using L. reuteri in bioconversion of glycerol to industrially important plate-form chemicals. L. reuteri grown on minimal media containing 1,2 propanediol was able to utilize the compound as a sole carbon and energy source. The growth of the bacteria was linear with time; however the specific growth rate was significantly low compared to bacteria grown on the same media in the presence of glucose.The fermentation of 1,2 propanediol by L. reuteri in presence and absence of glucose was followed for 72 h and the metabolites produced during the process were detected using HPLC. 1,2 Propanediol was completely converted to propionaldhyde in a time dependent fashion, this process had a higher rate in presence of glucose. Consequently the produced propionaldhyde was converted to propionic acid and propanol in a skewed equimolar manner. In presence of glucose: acetic acid, lactic acid, succinic acid and ethanol were detected while in absence of glucose only minute amounts of acetic acid and lactic acid were detected which indicates presence of different metabolic pathways for glucose and 1,2 propanediol metabolism. Resting cells of L. reuteri induced in presence of 1,2 propanediol have shown significant capabilities to convert aqueous glycerol to 1,3 propanediol, 3-hydroxypropionaldhyde and a compound proposed to be 3-hydroxypropionic acid as detected by gas chromatographic technique.</p>}},
author = {{Amin, Heba M. and Hashem, Abdelgawad M. and Ashour, Mohamed S. and Hatti-Kaul, Rajini}},
issn = {{1687-157X}},
keywords = {{1,2 Propanediol (1,2 PD); 1,3 Propanediol (1,3 PD); 3-Hydroxypropionaldehyde (3-HPA); Lactobacillus reuteri; Propionaldhyde; Propionic acid}},
language = {{eng}},
month = {{06}},
number = {{1}},
pages = {{53--59}},
publisher = {{Academy of Scientific Research and Technology}},
series = {{Journal of Genetic Engineering and Biotechnology}},
title = {{1,2 Propanediol utilization by Lactobacillus reuteri DSM 20016, role in bioconversion of glycerol to 1,3 propanediol, 3-hydroxypropionaldehyde and 3-hydroxypropionic acid}},
url = {{http://dx.doi.org/10.1016/j.jgeb.2012.12.002}},
doi = {{10.1016/j.jgeb.2012.12.002}},
volume = {{11}},
year = {{2013}},
}