Helicobacter pylori lipopolysaccharide in the IL-2 milieu activates lymphocytes from dyspeptic children
(2003) 5th International Workshop on Pathogenesis and Host Response in Helicobacter Infections 36(3). p.141-145- Abstract
- In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the... (More)
- In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the presence of IL-2, to IL-2 alone and to H. pylori LPS+IL-2. However, there was no difference in PHA-driven proliferation of PBML from the patients of those two groups. A negative correlation between the responsiveness to H. pylori LPS of PBML and occurrence of type B inflammation in gastric mucosa was demonstrated. The results suggest a contribution of H. pylori LPS to an outcome of H. pylori infection. It is speculated that H. pylori LPS by an activation of immunocompetent cells may reduce gastric inflammation, decrease bacterial load and prolong H. pylori infection. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/310897
- author
- Rudnicka, W ; Jarosinska, A ; Bak-Romaniszyn, L ; Moran, A ; Planeta-Malecka, I ; Wadström, Torkel LU and Chmiela, M
- organization
- publishing date
- 2003
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- LPS, IL-2, Helicobacter pylori, inflammation, cell proliferation
- host publication
- FEMS Immunology and Medical Microbiology (Helicobacter Pathogenesis and Immunology. Selected papers)
- volume
- 36
- issue
- 3
- pages
- 141 - 145
- publisher
- Elsevier
- conference name
- 5th International Workshop on Pathogenesis and Host Response in Helicobacter Infections
- conference dates
- 0001-01-02
- external identifiers
-
- wos:000182940400005
- pmid:12738383
- scopus:0038061766
- ISSN
- 2049-632X
- 0928-8244
- DOI
- 10.1016/S0928-8244(03)00023-3
- language
- English
- LU publication?
- yes
- id
- 7fae56d6-198a-4043-9617-ccc8eb2a2511 (old id 310897)
- date added to LUP
- 2016-04-01 12:10:35
- date last changed
- 2024-01-08 11:09:51
@inproceedings{7fae56d6-198a-4043-9617-ccc8eb2a2511,
abstract = {{In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the presence of IL-2, to IL-2 alone and to H. pylori LPS+IL-2. However, there was no difference in PHA-driven proliferation of PBML from the patients of those two groups. A negative correlation between the responsiveness to H. pylori LPS of PBML and occurrence of type B inflammation in gastric mucosa was demonstrated. The results suggest a contribution of H. pylori LPS to an outcome of H. pylori infection. It is speculated that H. pylori LPS by an activation of immunocompetent cells may reduce gastric inflammation, decrease bacterial load and prolong H. pylori infection. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.}},
author = {{Rudnicka, W and Jarosinska, A and Bak-Romaniszyn, L and Moran, A and Planeta-Malecka, I and Wadström, Torkel and Chmiela, M}},
booktitle = {{FEMS Immunology and Medical Microbiology (Helicobacter Pathogenesis and Immunology. Selected papers)}},
issn = {{2049-632X}},
keywords = {{LPS; IL-2; Helicobacter pylori; inflammation; cell proliferation}},
language = {{eng}},
number = {{3}},
pages = {{141--145}},
publisher = {{Elsevier}},
title = {{Helicobacter pylori lipopolysaccharide in the IL-2 milieu activates lymphocytes from dyspeptic children}},
url = {{http://dx.doi.org/10.1016/S0928-8244(03)00023-3}},
doi = {{10.1016/S0928-8244(03)00023-3}},
volume = {{36}},
year = {{2003}},
}