Continuous superporous agarose beds for chromatography and electrophoresis
(1999) In Journal of chromatography. A 832(1-2). p.29-39- Abstract
- Continuous agarose beds (monoliths) were prepared by casting agarose
emulsions designed to generate superporous agarose. The gel structures
obtained were transected by superpores (diameters could be varied in the
range 20–200 μm) through which liquids could be pumped. The pore
structure and the basic properties of the continuous gel were
investigated by microscopy and size exclusion chromatography. The
chromatographic behaviour was approximately the same as for beds packed
with homogeneous agarose beads with a particle diameter equivalent to
the distance between the superpores. In one application, the superporous
continuous agarose bed was derivatized with a NAD+ analogue
and... (More) - Continuous agarose beds (monoliths) were prepared by casting agarose
emulsions designed to generate superporous agarose. The gel structures
obtained were transected by superpores (diameters could be varied in the
range 20–200 μm) through which liquids could be pumped. The pore
structure and the basic properties of the continuous gel were
investigated by microscopy and size exclusion chromatography. The
chromatographic behaviour was approximately the same as for beds packed
with homogeneous agarose beads with a particle diameter equivalent to
the distance between the superpores. In one application, the superporous
continuous agarose bed was derivatized with a NAD+ analogue
and used in the affinity purification of bovine lactate dehydrogenase
from a crude extract. In another application, a new superporous
composite gel material was prepared by adding hydroxyapatite particles
to the agarose phase. The composite bed was used to separate a protein
mixture by hydroxyapatite chromatography. In a third application, the
continuous superporous agarose material was used as an electrophoresis
gel. Here, a water-immiscible organic liquid was pumped through the
superpores to dissipate the joule heat evolved, thus allowing high
current densities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7fce8762-1fa5-4771-ba07-92f8ad82996d
- author
- Gustavsson, Per-Erik LU and Larsson, Per-Olof LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Monolithic adsorbent, Affinity chromatography, Internally cooled electrophoresis gel, Lactate dehydrogenase, Hydroxyapatite particles
- in
- Journal of chromatography. A
- volume
- 832
- issue
- 1-2
- pages
- 11 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0032969578
- ISSN
- 0021-9673
- DOI
- 10.1016/S0021-9673(98)00979-0
- language
- English
- LU publication?
- yes
- id
- 7fce8762-1fa5-4771-ba07-92f8ad82996d
- date added to LUP
- 2024-05-18 21:26:23
- date last changed
- 2024-05-30 11:37:09
@article{7fce8762-1fa5-4771-ba07-92f8ad82996d, abstract = {{Continuous agarose beds (monoliths) were prepared by casting agarose <br> emulsions designed to generate superporous agarose. The gel structures <br> obtained were transected by superpores (diameters could be varied in the<br> range 20–200 μm) through which liquids could be pumped. The pore <br> structure and the basic properties of the continuous gel were <br> investigated by microscopy and size exclusion chromatography. The <br> chromatographic behaviour was approximately the same as for beds packed <br> with homogeneous agarose beads with a particle diameter equivalent to <br> the distance between the superpores. In one application, the superporous<br> continuous agarose bed was derivatized with a NAD<sup>+</sup> analogue <br> and used in the affinity purification of bovine lactate dehydrogenase <br> from a crude extract. In another application, a new superporous <br> composite gel material was prepared by adding hydroxyapatite particles <br> to the agarose phase. The composite bed was used to separate a protein <br> mixture by hydroxyapatite chromatography. In a third application, the <br> continuous superporous agarose material was used as an electrophoresis <br> gel. Here, a water-immiscible organic liquid was pumped through the <br> superpores to dissipate the joule heat evolved, thus allowing high <br> current densities.}}, author = {{Gustavsson, Per-Erik and Larsson, Per-Olof}}, issn = {{0021-9673}}, keywords = {{Monolithic adsorbent; Affinity chromatography; Internally cooled electrophoresis gel; Lactate dehydrogenase; Hydroxyapatite particles}}, language = {{eng}}, number = {{1-2}}, pages = {{29--39}}, publisher = {{Elsevier}}, series = {{Journal of chromatography. A}}, title = {{Continuous superporous agarose beds for chromatography and electrophoresis}}, url = {{http://dx.doi.org/10.1016/S0021-9673(98)00979-0}}, doi = {{10.1016/S0021-9673(98)00979-0}}, volume = {{832}}, year = {{1999}}, }