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Continuous superporous agarose beds for chromatography and electrophoresis

Gustavsson, Per-Erik LU and Larsson, Per-Olof LU (1999) In Journal of chromatography. A 832(1-2). p.29-39
Abstract
Continuous agarose beds (monoliths) were prepared by casting agarose
emulsions designed to generate superporous agarose. The gel structures
obtained were transected by superpores (diameters could be varied in the
range 20–200 μm) through which liquids could be pumped. The pore
structure and the basic properties of the continuous gel were
investigated by microscopy and size exclusion chromatography. The
chromatographic behaviour was approximately the same as for beds packed
with homogeneous agarose beads with a particle diameter equivalent to
the distance between the superpores. In one application, the superporous
continuous agarose bed was derivatized with a NAD+ analogue
and... (More)
Continuous agarose beds (monoliths) were prepared by casting agarose
emulsions designed to generate superporous agarose. The gel structures
obtained were transected by superpores (diameters could be varied in the
range 20–200 μm) through which liquids could be pumped. The pore
structure and the basic properties of the continuous gel were
investigated by microscopy and size exclusion chromatography. The
chromatographic behaviour was approximately the same as for beds packed
with homogeneous agarose beads with a particle diameter equivalent to
the distance between the superpores. In one application, the superporous
continuous agarose bed was derivatized with a NAD+ analogue
and used in the affinity purification of bovine lactate dehydrogenase
from a crude extract. In another application, a new superporous
composite gel material was prepared by adding hydroxyapatite particles
to the agarose phase. The composite bed was used to separate a protein
mixture by hydroxyapatite chromatography. In a third application, the
continuous superporous agarose material was used as an electrophoresis
gel. Here, a water-immiscible organic liquid was pumped through the
superpores to dissipate the joule heat evolved, thus allowing high
current densities. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Monolithic adsorbent, Affinity chromatography, Internally cooled electrophoresis gel, Lactate dehydrogenase, Hydroxyapatite particles
in
Journal of chromatography. A
volume
832
issue
1-2
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:0032969578
ISSN
0021-9673
DOI
10.1016/S0021-9673(98)00979-0
language
English
LU publication?
yes
id
7fce8762-1fa5-4771-ba07-92f8ad82996d
date added to LUP
2024-05-18 21:26:23
date last changed
2024-05-30 11:37:09
@article{7fce8762-1fa5-4771-ba07-92f8ad82996d,
  abstract     = {{Continuous agarose beds (monoliths) were prepared by casting agarose <br>
emulsions designed to generate superporous agarose. The gel structures <br>
obtained were transected by superpores (diameters could be varied in the<br>
 range 20–200 μm) through which liquids could be pumped. The pore <br>
structure and the basic properties of the continuous gel were <br>
investigated by microscopy and size exclusion chromatography. The <br>
chromatographic behaviour was approximately the same as for beds packed <br>
with homogeneous agarose beads with a particle diameter equivalent to <br>
the distance between the superpores. In one application, the superporous<br>
 continuous agarose bed was derivatized with a NAD<sup>+</sup> analogue <br>
and used in the affinity purification of bovine lactate dehydrogenase <br>
from a crude extract. In another application, a new superporous <br>
composite gel material was prepared by adding hydroxyapatite particles <br>
to the agarose phase. The composite bed was used to separate a protein <br>
mixture by hydroxyapatite chromatography. In a third application, the <br>
continuous superporous agarose material was used as an electrophoresis <br>
gel. Here, a water-immiscible organic liquid was pumped through the <br>
superpores to dissipate the joule heat evolved, thus allowing high <br>
current densities.}},
  author       = {{Gustavsson, Per-Erik and Larsson, Per-Olof}},
  issn         = {{0021-9673}},
  keywords     = {{Monolithic adsorbent; Affinity chromatography; Internally cooled electrophoresis gel; Lactate dehydrogenase; Hydroxyapatite particles}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{29--39}},
  publisher    = {{Elsevier}},
  series       = {{Journal of chromatography. A}},
  title        = {{Continuous superporous agarose beds for chromatography and electrophoresis}},
  url          = {{http://dx.doi.org/10.1016/S0021-9673(98)00979-0}},
  doi          = {{10.1016/S0021-9673(98)00979-0}},
  volume       = {{832}},
  year         = {{1999}},
}