Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis
(2022) In Neuro-Ophthalmology 46(4). p.215-219- Abstract
In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein... (More)
In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (μg/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41–5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10–1.67) or control rats (0.93, IQR 0.45–4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.
(Less)
- author
- Weissert, Robert ; Hugosson, Therése LU and Petzold, Axel
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- axonal degeneration, neurofilament protein, Optic neuritis, retina, tissue
- in
- Neuro-Ophthalmology
- volume
- 46
- issue
- 4
- pages
- 215 - 219
- publisher
- Taylor & Francis
- external identifiers
-
- scopus:85124070323
- pmid:35859627
- ISSN
- 0165-8107
- DOI
- 10.1080/01658107.2022.2025852
- language
- English
- LU publication?
- yes
- id
- 7fdcd30b-27f2-483a-8df6-5898bbb1ce72
- date added to LUP
- 2022-04-06 11:59:56
- date last changed
- 2024-06-19 23:58:08
@article{7fdcd30b-27f2-483a-8df6-5898bbb1ce72,
abstract = {{<p>In optic neuritis (ON), transient thickening of the macular retinal nerve fibre layer (RNFL) can be observed. This optical coherence tomography-based observation is not understood. The axonal diameter correlates with the neurofilament (Nf) protein content, but there are no data on the retinal tissue concentration of Nfs. The myelin-oligodendrocyte-glycoprotein (MOG) induced experimental autoimmune encephalomyelitis (EAE) model was used to investigate the retinas of Brown Norway rats with (i) visual evoked potentials (VEP) confirmed ON, (ii) VEP confirmed absence of ON and (iii) control animals. Twenty retinas were collected from MOG-EAE and control rats 27 days after immunisation. Retinal tissue Nf concentrations per total protein (μg/mg) were significantly higher in MOG-EAE rats with ON (median 4.29, interquartile range [IQR] 3.41–5.97) compared with MOG-EAE rats without ON (1.14, IQR 1.10–1.67) or control rats (0.93, IQR 0.45–4.00). The data suggest that up-regulation of Nf expression in the retinal ganglion cells precedes development of RNFL atrophy and plausibly explains the transient increase of axonal diameter and RNFL thickening.</p>}},
author = {{Weissert, Robert and Hugosson, Therése and Petzold, Axel}},
issn = {{0165-8107}},
keywords = {{axonal degeneration; neurofilament protein; Optic neuritis; retina; tissue}},
language = {{eng}},
number = {{4}},
pages = {{215--219}},
publisher = {{Taylor & Francis}},
series = {{Neuro-Ophthalmology}},
title = {{Upregulated Retinal Neurofilament Expression in Experimental Optic Neuritis}},
url = {{http://dx.doi.org/10.1080/01658107.2022.2025852}},
doi = {{10.1080/01658107.2022.2025852}},
volume = {{46}},
year = {{2022}},
}