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Nuclear magnetic resonance in conjunction with functional genomics suggests mitochondrial dysfunction in a murine model of cancer cachexia

Constantinou, Caterina; Fontes de Oliveira, Cibely LU ; Mintzopoulos, Dionyssios; Busquets, Silvia; He, Jianxin; Kesarwani, Meenu; Mindrinos, Michael N; Rahme, Laurence G; Argilés, Josep M and Tzika, A Aria (2011) In International Journal of Molecular Medicine 27(1). p.15-24
Abstract

Cancer patients commonly suffer from cachexia, a syndrome in which tumors induce metabolic changes in the host that lead to massive loss in skeletal muscle mass. Using a preclinical mouse model of cancer cachexia, we tested the hypothesis that tumor inoculation causes a reduction in ATP synthesis and genome-wide aberrant expression in skeletal muscle. Mice implanted with Lewis lung carcinomas were examined by in vivo 31P nuclear magnetic resonance (NMR). We examined ATP synthesis rate and the expression of genes that play key-regulatory roles in skeletal muscle metabolism. Our in vivo NMR results showed reduced ATP synthesis rate in tumor-bearing (TB) mice relative to control (C) mice, and were cross-validated with whole genome... (More)

Cancer patients commonly suffer from cachexia, a syndrome in which tumors induce metabolic changes in the host that lead to massive loss in skeletal muscle mass. Using a preclinical mouse model of cancer cachexia, we tested the hypothesis that tumor inoculation causes a reduction in ATP synthesis and genome-wide aberrant expression in skeletal muscle. Mice implanted with Lewis lung carcinomas were examined by in vivo 31P nuclear magnetic resonance (NMR). We examined ATP synthesis rate and the expression of genes that play key-regulatory roles in skeletal muscle metabolism. Our in vivo NMR results showed reduced ATP synthesis rate in tumor-bearing (TB) mice relative to control (C) mice, and were cross-validated with whole genome transcriptome data showing atypical expression levels of skeletal muscle regulatory genes such as peroxisomal proliferator activator receptor γ coactivator 1 ß (PGC-1ß), a major regulator of mitochondrial biogenesis and, mitochondrial uncoupling protein 3 (UCP3). Aberrant pattern of gene expression was also associated with genes involved in inflammation and immune response, protein and lipid catabolism, mitochondrial biogenesis and uncoupling, and inadequate oxidative stress defenses, and these effects led to cachexia. Our findings suggest that reduced ATP synthesis is linked to mitochondrial dysfunction, ultimately leading to skeletal muscle wasting and thus advance our understanding of skeletal muscle dysfunction suffered by cancer patients. This study represents a new line of research that can support the development of novel therapeutics in the molecular medicine of skeletal muscle wasting. Such therapeutics would have wide-spread applications not only for cancer patients, but also for many individuals suffering from other chronic or endstage diseases that exhibit muscle wasting, a condition for which only marginally effective treatments are currently available.

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publishing date
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Contribution to journal
publication status
published
keywords
Adenosine Triphosphate, Animals, Cachexia, Genomics, Humans, Magnetic Resonance Spectroscopy, Male, Mice, Mice, Inbred C57BL, Mitochondria, Muscle, Skeletal, Muscular Atrophy, Neoplasm Transplantation, Neoplasms, Random Allocation, Transcription Factors, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't
in
International Journal of Molecular Medicine
volume
27
issue
1
pages
10 pages
publisher
D.A. Spandidos
external identifiers
  • scopus:78651088003
ISSN
1791-244X
DOI
10.3892/ijmm.2010.557
language
English
LU publication?
no
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7fde7229-f12c-4b56-8883-e67bf1af9476
date added to LUP
2017-02-28 16:17:11
date last changed
2017-08-13 05:05:11
@article{7fde7229-f12c-4b56-8883-e67bf1af9476,
  abstract     = {<p>Cancer patients commonly suffer from cachexia, a syndrome in which tumors induce metabolic changes in the host that lead to massive loss in skeletal muscle mass. Using a preclinical mouse model of cancer cachexia, we tested the hypothesis that tumor inoculation causes a reduction in ATP synthesis and genome-wide aberrant expression in skeletal muscle. Mice implanted with Lewis lung carcinomas were examined by in vivo 31P nuclear magnetic resonance (NMR). We examined ATP synthesis rate and the expression of genes that play key-regulatory roles in skeletal muscle metabolism. Our in vivo NMR results showed reduced ATP synthesis rate in tumor-bearing (TB) mice relative to control (C) mice, and were cross-validated with whole genome transcriptome data showing atypical expression levels of skeletal muscle regulatory genes such as peroxisomal proliferator activator receptor γ coactivator 1 ß (PGC-1ß), a major regulator of mitochondrial biogenesis and, mitochondrial uncoupling protein 3 (UCP3). Aberrant pattern of gene expression was also associated with genes involved in inflammation and immune response, protein and lipid catabolism, mitochondrial biogenesis and uncoupling, and inadequate oxidative stress defenses, and these effects led to cachexia. Our findings suggest that reduced ATP synthesis is linked to mitochondrial dysfunction, ultimately leading to skeletal muscle wasting and thus advance our understanding of skeletal muscle dysfunction suffered by cancer patients. This study represents a new line of research that can support the development of novel therapeutics in the molecular medicine of skeletal muscle wasting. Such therapeutics would have wide-spread applications not only for cancer patients, but also for many individuals suffering from other chronic or endstage diseases that exhibit muscle wasting, a condition for which only marginally effective treatments are currently available.</p>},
  author       = {Constantinou, Caterina and Fontes de Oliveira, Cibely and Mintzopoulos, Dionyssios and Busquets, Silvia and He, Jianxin and Kesarwani, Meenu and Mindrinos, Michael N and Rahme, Laurence G and Argilés, Josep M and Tzika, A Aria},
  issn         = {1791-244X},
  keyword      = {Adenosine Triphosphate,Animals,Cachexia,Genomics,Humans,Magnetic Resonance Spectroscopy,Male,Mice,Mice, Inbred C57BL,Mitochondria,Muscle, Skeletal,Muscular Atrophy,Neoplasm Transplantation,Neoplasms,Random Allocation,Transcription Factors,Journal Article,Research Support, N.I.H., Extramural,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {15--24},
  publisher    = {D.A. Spandidos},
  series       = {International Journal of Molecular Medicine},
  title        = {Nuclear magnetic resonance in conjunction with functional genomics suggests mitochondrial dysfunction in a murine model of cancer cachexia},
  url          = {http://dx.doi.org/10.3892/ijmm.2010.557},
  volume       = {27},
  year         = {2011},
}