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Sodium Butyrate Enhancement of Protein Expression

Bassens, C ; Rigaux, P ; Hendrick, V ; Cherlet, M ; Sato, K ; Kotarsky, Knut LU and Werenne, J (2005) 2. p.625-627
Abstract
We studied in chimeric EGFP-Photinus luciferase HeLa cells the expression of fluorescence and luminescence in different cell culture conditions (medium with or without Serum, static or dynamic conditions, in suspension or on microcarriers) as a model to follow the productivity of a recombinant protein. By studying the effect of Butyrate known as general but unpredictable stimulating agent for protein expression, we were able to establish the optimal conditions for efficacy ( concentration, time of addition, ...)
Please use this url to cite or link to this publication:
author
; ; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
host publication
Animal Cell Technology Meets Genomics
volume
2
pages
625 - 627
publisher
Springer
ISBN
978-1-4020-2791-8
DOI
10.1007/1-4020-3103-3_126
language
English
LU publication?
yes
id
7ff79651-cec3-4884-9e94-cdef75379eff (old id 1132291)
date added to LUP
2016-04-04 10:28:41
date last changed
2018-11-21 20:58:59
@inbook{7ff79651-cec3-4884-9e94-cdef75379eff,
  abstract     = {{We studied in chimeric EGFP-Photinus luciferase HeLa cells the expression of fluorescence and luminescence in different cell culture conditions (medium with or without Serum, static or dynamic conditions, in suspension or on microcarriers) as a model to follow the productivity of a recombinant protein. By studying the effect of Butyrate known as general but unpredictable stimulating agent for protein expression, we were able to establish the optimal conditions for efficacy ( concentration, time of addition, ...)}},
  author       = {{Bassens, C and Rigaux, P and Hendrick, V and Cherlet, M and Sato, K and Kotarsky, Knut and Werenne, J}},
  booktitle    = {{Animal Cell Technology Meets Genomics}},
  isbn         = {{978-1-4020-2791-8}},
  language     = {{eng}},
  pages        = {{625--627}},
  publisher    = {{Springer}},
  title        = {{Sodium Butyrate Enhancement of Protein Expression}},
  url          = {{http://dx.doi.org/10.1007/1-4020-3103-3_126}},
  doi          = {{10.1007/1-4020-3103-3_126}},
  volume       = {{2}},
  year         = {{2005}},
}