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Purification, crystallization, and preliminary X-ray analysis of Bacillus subtilis ferrochelatase

Hansson, Mats LU and Al-Karadaghi, Salam LU (1995) In Proteins 23(4). p.607-609
Abstract
Bacillus subtilis ferrochelatase (EC 4.99.1.1), the final enzyme in protoheme IX biosynthesis, was produced with an inducible T7 RNA polymerase expression system in Escherichia coli and purified from the soluble cell fraction. It was crystallized from polyethylene glycol solution using the microseeding technique. The crystals diffract to a minimum Bragg spacing of 2.1 A. The space group is P4(2) with unit cell dimensions a = b = 50.2 A, c = 120.1 A.
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organization
publishing date
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Contribution to journal
publication status
published
subject
keywords
Genes, Ferrochelatase/*chemistry/isolation & purification, Escherichia coli, X-Ray, DNA-Directed RNA Polymerases, Crystallography, Crystallization, Molecular, Bacillus subtilis/*enzymology/genetics, Cloning, Bacterial, Plasmids, Polyethylene Glycols, *Protein Conformation, Recombinant Proteins/chemistry/isolation & purification, Viral Proteins
in
Proteins
volume
23
issue
4
pages
607 - 609
publisher
John Wiley & Sons
external identifiers
  • scopus:0029586760
ISSN
0887-3585
DOI
10.1002/prot.340230419
language
English
LU publication?
yes
id
7d6ea0ec-357e-46a5-93d4-394349558e18 (old id 8001513)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/8749860
date added to LUP
2015-10-01 10:12:38
date last changed
2017-01-01 07:09:34
@article{7d6ea0ec-357e-46a5-93d4-394349558e18,
  abstract     = {Bacillus subtilis ferrochelatase (EC 4.99.1.1), the final enzyme in protoheme IX biosynthesis, was produced with an inducible T7 RNA polymerase expression system in Escherichia coli and purified from the soluble cell fraction. It was crystallized from polyethylene glycol solution using the microseeding technique. The crystals diffract to a minimum Bragg spacing of 2.1 A. The space group is P4(2) with unit cell dimensions a = b = 50.2 A, c = 120.1 A.},
  author       = {Hansson, Mats and Al-Karadaghi, Salam},
  issn         = {0887-3585},
  keyword      = {Genes,Ferrochelatase/*chemistry/isolation & purification,Escherichia coli,X-Ray,DNA-Directed RNA Polymerases,Crystallography,Crystallization,Molecular,Bacillus subtilis/*enzymology/genetics,Cloning,Bacterial,Plasmids,Polyethylene Glycols,*Protein Conformation,Recombinant Proteins/chemistry/isolation & purification,Viral Proteins},
  language     = {eng},
  number       = {4},
  pages        = {607--609},
  publisher    = {John Wiley & Sons},
  series       = {Proteins},
  title        = {Purification, crystallization, and preliminary X-ray analysis of Bacillus subtilis ferrochelatase},
  url          = {http://dx.doi.org/10.1002/prot.340230419},
  volume       = {23},
  year         = {1995},
}