Advanced

Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway

Muller, A. H.; Gough, S. P.; Bollivar, D. W.; Meldal, M.; Willows, R. D. and Hansson, Mats LU (2011) In Analytical Biochemistry 419(2). p.271-276
Abstract
Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid... (More)
Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Hordeum/metabolism, Culture Media/chemistry, Thin Layer, Chromatography, Chlorophyllides/*chemical synthesis/chemistry/isolation & purification, *Biosynthetic Pathways, Absorption, Biochemistry/*methods, Magnesium/metabolism, Polysorbates, Rhodobacter capsulatus/metabolism, Spectrum Analysis
in
Analytical Biochemistry
volume
419
issue
2
pages
271 - 276
publisher
Elsevier
external identifiers
  • scopus:80054056008
ISSN
1096-0309
DOI
10.1016/j.ab.2011.08.028
language
English
LU publication?
no
id
c2d2fb32-0de5-400f-8baf-4990920f5258 (old id 8001602)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/21925479
date added to LUP
2015-09-30 13:14:12
date last changed
2017-01-01 03:00:41
@article{c2d2fb32-0de5-400f-8baf-4990920f5258,
  abstract     = {Chlorophyllide a is a metabolite late in the biosynthesis of chlorophylls and bacteriochlorophylls. Isolation procedures for chlorophyllide a from Rhodobacter capsulatus CB1200 and barley (Hordeum vulgare L.) are described and compared. R. capsulatus CB1200 is a double mutant in the bacteriochlorophyllide a biosynthetic pathway, and chlorophyllide a is excreted by the cells when grown in Tween 80-containing liquid medium. It was purified by liquid or solid phase extraction, yielding 7 mg of chlorophyllide a from 1 L of culture. In a second approach, intrinsic chlorophyllase activity was used to dephytylate chlorophyll in an acetonic preparation of leaves of wild-type or chlorophyll b-deficient barley. Purification was achieved by liquid phase extraction, yielding 14 mug of chlorophyllide a per gram of barley leaves. Chlorophyllide a was identified by thin layer chromatography, absorption spectroscopy, and mass spectrometry.},
  author       = {Muller, A. H. and Gough, S. P. and Bollivar, D. W. and Meldal, M. and Willows, R. D. and Hansson, Mats},
  issn         = {1096-0309},
  keyword      = {Hordeum/metabolism,Culture Media/chemistry,Thin Layer,Chromatography,Chlorophyllides/*chemical synthesis/chemistry/isolation & purification,*Biosynthetic Pathways,Absorption,Biochemistry/*methods,Magnesium/metabolism,Polysorbates,Rhodobacter capsulatus/metabolism,Spectrum Analysis},
  language     = {eng},
  number       = {2},
  pages        = {271--276},
  publisher    = {Elsevier},
  series       = {Analytical Biochemistry},
  title        = {Methods for the preparation of chlorophyllide a: an intermediate of the chlorophyll biosynthetic pathway},
  url          = {http://dx.doi.org/10.1016/j.ab.2011.08.028},
  volume       = {419},
  year         = {2011},
}