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Characterization of mutations in barley fch2 encoding chlorophyllide a oxygenase

Mueller, A. H.; Dockter, C.; Gough, S. P.; Lundqvist, U.; von Wettstein, D. and Hansson, Mats LU (2012) In Plant and Cell Physiology 53(7). p.1232-1246
Abstract
The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the gene for chlorophyllide a oxygenase. A five-base insertion was found in fch2 and base deletions in clo-f2.101, clo-f2.105, clo-f2.2800 and clo-f2.3613. In clo-f2.105 and clo-f2.108, nonsense base exchanges were discovered. All of these mutations led to a premature stop of translation and none of the mutants formed Chl b. The mutant clo-f2.2807... (More)
The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the gene for chlorophyllide a oxygenase. A five-base insertion was found in fch2 and base deletions in clo-f2.101, clo-f2.105, clo-f2.2800 and clo-f2.3613. In clo-f2.105 and clo-f2.108, nonsense base exchanges were discovered. All of these mutations led to a premature stop of translation and none of the mutants formed Chl b. The mutant clo-f2.2807 was transcript deficient and formed no Chl b. Missense mutations in clo-f2.102 (leading to the amino acid exchange D495N) and clo-f2.103 (G280D) resulted in a total lack of Chl b, whereas in the missense mutants clo-f2.107 (P419L), clo-f2.109 (A94T), clo-f2.122 (C320Y), clo-f2.123 (A94T), clo-f2.133 (A376V) and clo-f2.181 (L373F) intermediate contents of Chl b were determined. The missense mutations affect conserved residues, and their effect on chlorophyllide a oxygenase is discussed. The mutations in clo-f2.102, clo-f2.103, clo-f2.133 and clo-f2.181 may influence electron transfer as illustrated in the active site of a structural model protein. The changes in clo-f2.107, clo-f2.109, clo-f2.122 and clo-f2.123 may lead to Chlb deficiency by interfering with the regulation of chlorophyllide a oxygenase. The correlation of mutations and phenotypes strongly supports that the barley locus fch2 encodes chlorophyllide a oxygenase. (Less)
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subject
keywords
Hordeum/enzymology/*genetics, Genes, Plant, Enzymologic, Gene Expression Regulation, Frameshift Mutation, Electron Transport, Nonsense/genetics/metabolism, Codon, Molecular, Cloning, Plant/genetics/metabolism, Chromosomes, Chromosome Mapping, Chlorophyll/genetics/metabolism, Catalytic Domain, Alleles, Amino Acid Sequence, Molecular Sequence Data, *Mutation, Missense, Oxygenases/*genetics/metabolism, Phenotype, Plant Proteins/genetics/*metabolism, Protein Structure, Tertiary, Sequence Alignment, Synteny
in
Plant and Cell Physiology
volume
53
issue
7
pages
1232 - 1246
publisher
Oxford University Press
external identifiers
  • scopus:84863938407
ISSN
1471-9053
DOI
10.1093/pcp/pcs062
language
English
LU publication?
no
id
f7867a0d-bda9-436c-8d78-a9e2c93ea2cd (old id 8001631)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22537757
date added to LUP
2015-09-30 14:12:31
date last changed
2017-07-23 03:21:59
@article{f7867a0d-bda9-436c-8d78-a9e2c93ea2cd,
  abstract     = {The barley (Hordeum vulgare L.) mutants fch2 and clo-f2 comprise an allelic group of 14 Chl b-deficient lines. The genetic map position of fch2 corresponds to the physical map position of the gene encoding chlorophyllide a oxygenase. This enzyme converts chlorophyllide a to chlorophyllide b and it is essential for Chl b biosynthesis. The fch2 and clo-f2 barley lines were shown to be mutated in the gene for chlorophyllide a oxygenase. A five-base insertion was found in fch2 and base deletions in clo-f2.101, clo-f2.105, clo-f2.2800 and clo-f2.3613. In clo-f2.105 and clo-f2.108, nonsense base exchanges were discovered. All of these mutations led to a premature stop of translation and none of the mutants formed Chl b. The mutant clo-f2.2807 was transcript deficient and formed no Chl b. Missense mutations in clo-f2.102 (leading to the amino acid exchange D495N) and clo-f2.103 (G280D) resulted in a total lack of Chl b, whereas in the missense mutants clo-f2.107 (P419L), clo-f2.109 (A94T), clo-f2.122 (C320Y), clo-f2.123 (A94T), clo-f2.133 (A376V) and clo-f2.181 (L373F) intermediate contents of Chl b were determined. The missense mutations affect conserved residues, and their effect on chlorophyllide a oxygenase is discussed. The mutations in clo-f2.102, clo-f2.103, clo-f2.133 and clo-f2.181 may influence electron transfer as illustrated in the active site of a structural model protein. The changes in clo-f2.107, clo-f2.109, clo-f2.122 and clo-f2.123 may lead to Chlb deficiency by interfering with the regulation of chlorophyllide a oxygenase. The correlation of mutations and phenotypes strongly supports that the barley locus fch2 encodes chlorophyllide a oxygenase.},
  author       = {Mueller, A. H. and Dockter, C. and Gough, S. P. and Lundqvist, U. and von Wettstein, D. and Hansson, Mats},
  issn         = {1471-9053},
  keyword      = {Hordeum/enzymology/*genetics,Genes,Plant,Enzymologic,Gene Expression Regulation,Frameshift Mutation,Electron Transport,Nonsense/genetics/metabolism,Codon,Molecular,Cloning,Plant/genetics/metabolism,Chromosomes,Chromosome Mapping,Chlorophyll/genetics/metabolism,Catalytic Domain,Alleles,Amino Acid Sequence,Molecular Sequence Data,*Mutation,Missense,Oxygenases/*genetics/metabolism,Phenotype,Plant Proteins/genetics/*metabolism,Protein Structure,Tertiary,Sequence Alignment,Synteny},
  language     = {eng},
  number       = {7},
  pages        = {1232--1246},
  publisher    = {Oxford University Press},
  series       = {Plant and Cell Physiology},
  title        = {Characterization of mutations in barley fch2 encoding chlorophyllide a oxygenase},
  url          = {http://dx.doi.org/10.1093/pcp/pcs062},
  volume       = {53},
  year         = {2012},
}