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Induced variations in brassinosteroid genes define barley height and sturdiness, and expand the green revolution genetic toolkit

Dockter, C.; Gruszka, D.; Braumann, I.; Druka, A.; Druka, I.; Franckowiak, J.; Gough, S. P.; Janeczko, A.; Kurowska, M. and Lundqvist, J., et al. (2014) In Plant Physiology 166(4). p.1912-1927
Abstract
Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related... (More)
Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROID-INSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroid-related genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars. (Less)
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published
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Plant Physiology
volume
166
issue
4
pages
1912 - 1927
publisher
American Society of Plant Biologists
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  • scopus:84914146005
ISSN
1532-2548
DOI
10.1104/pp.114.250738
language
English
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no
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9c0be234-aefa-4a86-b611-3cc4b8511f48 (old id 8001703)
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http://www.ncbi.nlm.nih.gov/pubmed/25332507
date added to LUP
2015-09-30 09:44:03
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2017-08-27 05:46:05
@article{9c0be234-aefa-4a86-b611-3cc4b8511f48,
  abstract     = {Reduced plant height and culm robustness are quantitative characteristics important for assuring cereal crop yield and quality under adverse weather conditions. A very limited number of short-culm mutant alleles were introduced into commercial crop cultivars during the Green Revolution. We identified phenotypic traits, including sturdy culm, specific for deficiencies in brassinosteroid biosynthesis and signaling in semidwarf mutants of barley (Hordeum vulgare). This set of characteristic traits was explored to perform a phenotypic screen of near-isogenic short-culm mutant lines from the brachytic, breviaristatum, dense spike, erectoides, semibrachytic, semidwarf, and slender dwarf mutant groups. In silico mapping of brassinosteroid-related genes in the barley genome in combination with sequencing of barley mutant lines assigned more than 20 historic mutants to three brassinosteroid-biosynthesis genes (BRASSINOSTEROID-6-OXIDASE, CONSTITUTIVE PHOTOMORPHOGENIC DWARF, and DIMINUTO) and one brassinosteroid-signaling gene (BRASSINOSTEROID-INSENSITIVE1 [HvBRI1]). Analyses of F2 and M2 populations, allelic crosses, and modeling of nonsynonymous amino acid exchanges in protein crystal structures gave a further understanding of the control of barley plant architecture and sturdiness by brassinosteroid-related genes. Alternatives to the widely used but highly temperature-sensitive uzu1.a allele of HvBRI1 represent potential genetic building blocks for breeding strategies with sturdy and climate-tolerant barley cultivars.},
  author       = {Dockter, C. and Gruszka, D. and Braumann, I. and Druka, A. and Druka, I. and Franckowiak, J. and Gough, S. P. and Janeczko, A. and Kurowska, M. and Lundqvist, J. and Lundqvist, U. and Marzec, M. and Matyszczak, I. and Muller, A. H. and Oklestkova, J. and Schulz, B. and Zakhrabekova, Shakhira and Hansson, Mats},
  issn         = {1532-2548},
  language     = {eng},
  number       = {4},
  pages        = {1912--1927},
  publisher    = {American Society of Plant Biologists},
  series       = {Plant Physiology},
  title        = {Induced variations in brassinosteroid genes define barley height and sturdiness, and expand the green revolution genetic toolkit},
  url          = {http://dx.doi.org/10.1104/pp.114.250738},
  volume       = {166},
  year         = {2014},
}