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Does human papillomavirus-negative condylomata exist?

Arroyo Mühr, Laila Sara; Bzhalava, Davit; Lagheden, Camilla; Eklund, Carina; Johansson, Hanna K LU ; Forslund, Ola LU ; Dillner, Joakim and Hultin, Emilie (2015) In Virology 485. p.283-288
Abstract
Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deeper metagenomics sequencing. Sequencing of whole genome amplified DNA from 40 apparently "HPV-negative" condylomata detected HPV in 37/40 specimens. We found 75 different HPV types, out of which 43 represented novel putative HPV types. Three types were cloned and established as HPV types 200, 201 and 202. Molluscum contagiosum virus was detected in 24 of the 40 samples. In summary, deep sequencing enables detection of HPV in... (More)
Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deeper metagenomics sequencing. Sequencing of whole genome amplified DNA from 40 apparently "HPV-negative" condylomata detected HPV in 37/40 specimens. We found 75 different HPV types, out of which 43 represented novel putative HPV types. Three types were cloned and established as HPV types 200, 201 and 202. Molluscum contagiosum virus was detected in 24 of the 40 samples. In summary, deep sequencing enables detection of HPV in almost all condylomata. "HPV-negative" condylomata might largely be explained by clinical misdiagnosis or the presence of viral variants, distantly related HPV types and/or low viral loads. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Virology
volume
485
pages
283 - 288
publisher
Elsevier
external identifiers
  • pmid:26318260
  • wos:000363993100029
  • scopus:84940094788
ISSN
1096-0341
DOI
10.1016/j.virol.2015.07.023
language
English
LU publication?
yes
id
ad4cf078-a3d7-4357-9e11-3fd1c1a7fb17 (old id 8043800)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26318260?dopt=Abstract
date added to LUP
2015-10-07 21:49:20
date last changed
2017-10-01 03:00:35
@article{ad4cf078-a3d7-4357-9e11-3fd1c1a7fb17,
  abstract     = {Condylomata acuminata is caused by human papillomavirus (HPV). PCR with consensus primers will typically detect HPV in >96% of condylomata. Metagenomic sequencing has found that some "HPV-negative" condylomata do indeed contain HPV. We wished to perform a renewed evaluation of the "HPV-negative" condylomata using deeper metagenomics sequencing. Sequencing of whole genome amplified DNA from 40 apparently "HPV-negative" condylomata detected HPV in 37/40 specimens. We found 75 different HPV types, out of which 43 represented novel putative HPV types. Three types were cloned and established as HPV types 200, 201 and 202. Molluscum contagiosum virus was detected in 24 of the 40 samples. In summary, deep sequencing enables detection of HPV in almost all condylomata. "HPV-negative" condylomata might largely be explained by clinical misdiagnosis or the presence of viral variants, distantly related HPV types and/or low viral loads.},
  author       = {Arroyo Mühr, Laila Sara and Bzhalava, Davit and Lagheden, Camilla and Eklund, Carina and Johansson, Hanna K and Forslund, Ola and Dillner, Joakim and Hultin, Emilie},
  issn         = {1096-0341},
  language     = {eng},
  pages        = {283--288},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Does human papillomavirus-negative condylomata exist?},
  url          = {http://dx.doi.org/10.1016/j.virol.2015.07.023},
  volume       = {485},
  year         = {2015},
}