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Acoustophoretic removal of proteins from blood components.

Tenje, Maria; Lundgren, Maria LU ; Swärd-Nilsson, Ann-Margret; Kjeldsen-Kragh, Jens LU ; Lyxe, Lena and Lenshof, Andreas (2015) In Biomedical Microdevices 17(5). p.95-95
Abstract
This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC... (More)
This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomedical Microdevices
volume
17
issue
5
pages
95 - 95
publisher
Springer
external identifiers
  • pmid:26315975
  • wos:000362281200012
  • scopus:84940501940
ISSN
1572-8781
DOI
10.1007/s10544-015-0003-5
language
English
LU publication?
yes
id
129b4f5f-c4a9-4a9c-a072-6190e928367b (old id 8043838)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26315975?dopt=Abstract
date added to LUP
2015-10-07 17:56:57
date last changed
2017-01-01 04:18:39
@article{129b4f5f-c4a9-4a9c-a072-6190e928367b,
  abstract     = {This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.},
  author       = {Tenje, Maria and Lundgren, Maria and Swärd-Nilsson, Ann-Margret and Kjeldsen-Kragh, Jens and Lyxe, Lena and Lenshof, Andreas},
  issn         = {1572-8781},
  language     = {eng},
  number       = {5},
  pages        = {95--95},
  publisher    = {Springer},
  series       = {Biomedical Microdevices},
  title        = {Acoustophoretic removal of proteins from blood components.},
  url          = {http://dx.doi.org/10.1007/s10544-015-0003-5},
  volume       = {17},
  year         = {2015},
}