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Acoustophoretic removal of proteins from blood components.

Tenje, Maria ; Lundgren, Maria LU ; Swärd-Nilsson, Ann-Margret ; Kjeldsen-Kragh, Jens LU ; Lyxe, Lena and Lenshof, Andreas LU orcid (2015) In Biomedical Microdevices 17(5). p.95-95
Abstract
This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC... (More)
This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomedical Microdevices
volume
17
issue
5
pages
95 - 95
publisher
Springer
external identifiers
  • pmid:26315975
  • wos:000362281200012
  • scopus:84940501940
  • pmid:26315975
ISSN
1572-8781
DOI
10.1007/s10544-015-0003-5
language
English
LU publication?
yes
id
129b4f5f-c4a9-4a9c-a072-6190e928367b (old id 8043838)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26315975?dopt=Abstract
date added to LUP
2016-04-01 11:16:26
date last changed
2024-11-05 02:39:08
@article{129b4f5f-c4a9-4a9c-a072-6190e928367b,
  abstract     = {{This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97 %, compared to just above 90 % of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.}},
  author       = {{Tenje, Maria and Lundgren, Maria and Swärd-Nilsson, Ann-Margret and Kjeldsen-Kragh, Jens and Lyxe, Lena and Lenshof, Andreas}},
  issn         = {{1572-8781}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{95--95}},
  publisher    = {{Springer}},
  series       = {{Biomedical Microdevices}},
  title        = {{Acoustophoretic removal of proteins from blood components.}},
  url          = {{http://dx.doi.org/10.1007/s10544-015-0003-5}},
  doi          = {{10.1007/s10544-015-0003-5}},
  volume       = {{17}},
  year         = {{2015}},
}