Recipient T lymphocytes modulate the severity of antibody-mediated transfusion-related acute lung injury
(2010) In Blood 116(16). p.9-3073- Abstract
Transfusion-related acute lung injury (TRALI) is a serious complication of transfusion and has been ranked as one of the leading causes of transfusion-related fatalities. Nonetheless, many details of the immunopathogenesis of TRALI, particularly with respect to recipient factors are unknown. We used a murine model of antibody-mediated TRALI in an attempt to understand the role that recipient lymphocytes might play in TRALI reactions. Intravenous injection of an IgG2a antimurine major histocompatibility complex class I antibody (34-1-2s) into BALB/c mice induced moderate hypothermia and pulmonary granulocyte accumulation but no pulmonary edema nor mortality. In contrast, 34-1-2s injections into mice with severe combined immunodeficiency... (More)
Transfusion-related acute lung injury (TRALI) is a serious complication of transfusion and has been ranked as one of the leading causes of transfusion-related fatalities. Nonetheless, many details of the immunopathogenesis of TRALI, particularly with respect to recipient factors are unknown. We used a murine model of antibody-mediated TRALI in an attempt to understand the role that recipient lymphocytes might play in TRALI reactions. Intravenous injection of an IgG2a antimurine major histocompatibility complex class I antibody (34-1-2s) into BALB/c mice induced moderate hypothermia and pulmonary granulocyte accumulation but no pulmonary edema nor mortality. In contrast, 34-1-2s injections into mice with severe combined immunodeficiency caused severe hypothermia, severe pulmonary edema, and approximately 40% mortality indicating a critical role for T and B lymphocytes in suppressing TRALI reactions. Adoptive transfer of purified CD8(+) T lymphocytes or CD4(+) T cells but not CD19(+) B cells into the severe combined immunodeficiency mice alleviated the antibody-induced hypothermia, lung damage, and mortality, suggesting that T lymphocytes were responsible for the protective effect. Taken together, these results suggest that recipient T lymphocytes play a significant role in suppressing antibody-mediated TRALI reactions. They identify a potentially new recipient mechanism that controls the severity of TRALI reactions.
(Less)
- author
- Fung, Yoke Lin ; Kim, Michael ; Tabuchi, Arata ; Aslam, Rukhsana ; Speck, Edwin R ; Chow, Leola ; Kuebler, Wolfgang M ; Freedman, John and Semple, John W LU
- publishing date
- 2010-10-21
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acute Lung Injury, Animals, Antibodies, Blood Transfusion, Chemokine CXCL2, Histocompatibility Antigens Class I, Hypothermia, Immunoglobulin G, Lung, Lymphocyte Transfusion, Male, Mice, Mice, Inbred BALB C, Mice, SCID, Neutrophils, T-Lymphocytes, Journal Article, Research Support, Non-U.S. Gov't
- in
- Blood
- volume
- 116
- issue
- 16
- pages
- 7 pages
- publisher
- American Society of Hematology
- external identifiers
-
- pmid:20616220
- scopus:77958171596
- ISSN
- 1528-0020
- DOI
- 10.1182/blood-2010-05-284570
- language
- English
- LU publication?
- no
- id
- 8050c716-0bfe-4d13-8900-d80993466663
- date added to LUP
- 2016-09-23 12:05:43
- date last changed
- 2024-04-05 07:01:22
@article{8050c716-0bfe-4d13-8900-d80993466663,
abstract = {{<p>Transfusion-related acute lung injury (TRALI) is a serious complication of transfusion and has been ranked as one of the leading causes of transfusion-related fatalities. Nonetheless, many details of the immunopathogenesis of TRALI, particularly with respect to recipient factors are unknown. We used a murine model of antibody-mediated TRALI in an attempt to understand the role that recipient lymphocytes might play in TRALI reactions. Intravenous injection of an IgG2a antimurine major histocompatibility complex class I antibody (34-1-2s) into BALB/c mice induced moderate hypothermia and pulmonary granulocyte accumulation but no pulmonary edema nor mortality. In contrast, 34-1-2s injections into mice with severe combined immunodeficiency caused severe hypothermia, severe pulmonary edema, and approximately 40% mortality indicating a critical role for T and B lymphocytes in suppressing TRALI reactions. Adoptive transfer of purified CD8(+) T lymphocytes or CD4(+) T cells but not CD19(+) B cells into the severe combined immunodeficiency mice alleviated the antibody-induced hypothermia, lung damage, and mortality, suggesting that T lymphocytes were responsible for the protective effect. Taken together, these results suggest that recipient T lymphocytes play a significant role in suppressing antibody-mediated TRALI reactions. They identify a potentially new recipient mechanism that controls the severity of TRALI reactions.</p>}},
author = {{Fung, Yoke Lin and Kim, Michael and Tabuchi, Arata and Aslam, Rukhsana and Speck, Edwin R and Chow, Leola and Kuebler, Wolfgang M and Freedman, John and Semple, John W}},
issn = {{1528-0020}},
keywords = {{Acute Lung Injury; Animals; Antibodies; Blood Transfusion; Chemokine CXCL2; Histocompatibility Antigens Class I; Hypothermia; Immunoglobulin G; Lung; Lymphocyte Transfusion; Male; Mice; Mice, Inbred BALB C; Mice, SCID; Neutrophils; T-Lymphocytes; Journal Article; Research Support, Non-U.S. Gov't}},
language = {{eng}},
month = {{10}},
number = {{16}},
pages = {{9--3073}},
publisher = {{American Society of Hematology}},
series = {{Blood}},
title = {{Recipient T lymphocytes modulate the severity of antibody-mediated transfusion-related acute lung injury}},
url = {{http://dx.doi.org/10.1182/blood-2010-05-284570}},
doi = {{10.1182/blood-2010-05-284570}},
volume = {{116}},
year = {{2010}},
}