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Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes

Bozorgzadeh, Somayyeh LU ; Hamidi, Hassan LU ; Ortiz, Roberto LU ; Ludwig, Roland and Gorton, Lo LU (2015) In Physical Chemistry Chemical Physics 17(37). p.24157-24165
Abstract
In the present work, platinum and palladium nanoparticles (PtNPs and PdNPs) were decorated on the surface of multi-walled carbon nanotubes (MWCNTs) by a simple thermal decomposition method. The prepared nanohybrids, PtNPs-MWCNTs and PdNPs-MWCNTs, were cast on the surface of spectrographic graphite electrodes and then Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) was adsorbed on the modified layer. Direct electron transfer between PcCDH and the nanostructured modified electrodes was studied using flow injection amperometry and cyclic voltammetry. The maximum current responses (I-max) and the apparent Michaelis-Menten constants (K-M(app)) for the different PcCDH modified electrodes were calculated by fitting the data to the... (More)
In the present work, platinum and palladium nanoparticles (PtNPs and PdNPs) were decorated on the surface of multi-walled carbon nanotubes (MWCNTs) by a simple thermal decomposition method. The prepared nanohybrids, PtNPs-MWCNTs and PdNPs-MWCNTs, were cast on the surface of spectrographic graphite electrodes and then Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) was adsorbed on the modified layer. Direct electron transfer between PcCDH and the nanostructured modified electrodes was studied using flow injection amperometry and cyclic voltammetry. The maximum current responses (I-max) and the apparent Michaelis-Menten constants (K-M(app)) for the different PcCDH modified electrodes were calculated by fitting the data to the Michaelis-Menten equation and compared. The sensitivity towards lactose was 3.07 and 3.28 mu A mM(-1) at the PcCDH/PtNPs-MWCNTs/SPGE and PcCDH/PdNPs-MWCNTs/SPGE electrodes, respectively, which were higher than those measured at the PcCDH/MWCNTs/SPGE (2.60 mu A mM(-1)) and PcCDH/SPGE (0.92 mu A mM(-1)). The modified electrodes were additionally tested as bioanodes for biofuel cell applications. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Physical Chemistry Chemical Physics
volume
17
issue
37
pages
24157 - 24165
publisher
Royal Society of Chemistry
external identifiers
  • pmid:26323551
  • wos:000361543200052
  • scopus:84941754827
ISSN
1463-9084
DOI
10.1039/c5cp03812j
language
English
LU publication?
yes
id
3f44338e-406b-4fe5-899c-7dd1c89bde0f (old id 8071055)
date added to LUP
2015-10-22 08:51:31
date last changed
2017-09-24 04:15:35
@article{3f44338e-406b-4fe5-899c-7dd1c89bde0f,
  abstract     = {In the present work, platinum and palladium nanoparticles (PtNPs and PdNPs) were decorated on the surface of multi-walled carbon nanotubes (MWCNTs) by a simple thermal decomposition method. The prepared nanohybrids, PtNPs-MWCNTs and PdNPs-MWCNTs, were cast on the surface of spectrographic graphite electrodes and then Phanerochaete chrysosporium cellobiose dehydrogenase (PcCDH) was adsorbed on the modified layer. Direct electron transfer between PcCDH and the nanostructured modified electrodes was studied using flow injection amperometry and cyclic voltammetry. The maximum current responses (I-max) and the apparent Michaelis-Menten constants (K-M(app)) for the different PcCDH modified electrodes were calculated by fitting the data to the Michaelis-Menten equation and compared. The sensitivity towards lactose was 3.07 and 3.28 mu A mM(-1) at the PcCDH/PtNPs-MWCNTs/SPGE and PcCDH/PdNPs-MWCNTs/SPGE electrodes, respectively, which were higher than those measured at the PcCDH/MWCNTs/SPGE (2.60 mu A mM(-1)) and PcCDH/SPGE (0.92 mu A mM(-1)). The modified electrodes were additionally tested as bioanodes for biofuel cell applications.},
  author       = {Bozorgzadeh, Somayyeh and Hamidi, Hassan and Ortiz, Roberto and Ludwig, Roland and Gorton, Lo},
  issn         = {1463-9084},
  language     = {eng},
  number       = {37},
  pages        = {24157--24165},
  publisher    = {Royal Society of Chemistry},
  series       = {Physical Chemistry Chemical Physics},
  title        = {Direct electron transfer of Phanerochaete chrysosporium cellobiose dehydrogenase at platinum and palladium nanoparticles decorated carbon nanotubes modified electrodes},
  url          = {http://dx.doi.org/10.1039/c5cp03812j},
  volume       = {17},
  year         = {2015},
}