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Separation of Nucleic Acids Using Single- and Multimodal Chromatography

Matos, Tiago LU and Bülow, Leif LU (2019) In Current protein & peptide science 20(1). p.49-55
Abstract

The needs for purified nucleic acids for preparative and analytical applications have increased constantly, demanding for the development of new and more efficient methods for their recovery and isolation. DNA molecules harbour some intrinsic chemical properties that render them suitable for chromatographic separations. These include a negatively charged phosphate backbone as well as a hydrophobic character originating mainly from the major groove of DNA which exposes the base pairs on the surface of the molecule. In addition, single stranded DNA often allows for a free exposure of the hydrophobic aromatic bases. In this review, multimodal chromatography (MMC) has been evaluated as an alternative tool for complex separations of nucleic... (More)

The needs for purified nucleic acids for preparative and analytical applications have increased constantly, demanding for the development of new and more efficient methods for their recovery and isolation. DNA molecules harbour some intrinsic chemical properties that render them suitable for chromatographic separations. These include a negatively charged phosphate backbone as well as a hydrophobic character originating mainly from the major groove of DNA which exposes the base pairs on the surface of the molecule. In addition, single stranded DNA often allows for a free exposure of the hydrophobic aromatic bases. In this review, multimodal chromatography (MMC) has been evaluated as an alternative tool for complex separations of nucleic acids. MMC embraces more than one kind of interaction between the chromatographic ligand and the target molecules. These resins have often proved superior to conventional single-mode chromatographic materials for DNA isolation, including, e.g., the purification of plasmid DNA from crude cell lysates and for the preparation of DNA fragments before or after a polymerase chain reaction (PCR).

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Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
anion exchange, chromatography, DNA, elution, Hydrophobic interactions, multimodal, plasmid.
in
Current protein & peptide science
volume
20
issue
1
pages
7 pages
publisher
Bentham Science Publishers
external identifiers
  • scopus:85056617319
ISSN
1875-5550
DOI
10.2174/1389203718666171024112556
language
English
LU publication?
yes
id
80904e63-6fc1-4e25-bc59-f49f4dd516aa
date added to LUP
2018-11-26 12:43:47
date last changed
2019-02-03 05:37:08
@article{80904e63-6fc1-4e25-bc59-f49f4dd516aa,
  abstract     = {<p>The needs for purified nucleic acids for preparative and analytical applications have increased constantly, demanding for the development of new and more efficient methods for their recovery and isolation. DNA molecules harbour some intrinsic chemical properties that render them suitable for chromatographic separations. These include a negatively charged phosphate backbone as well as a hydrophobic character originating mainly from the major groove of DNA which exposes the base pairs on the surface of the molecule. In addition, single stranded DNA often allows for a free exposure of the hydrophobic aromatic bases. In this review, multimodal chromatography (MMC) has been evaluated as an alternative tool for complex separations of nucleic acids. MMC embraces more than one kind of interaction between the chromatographic ligand and the target molecules. These resins have often proved superior to conventional single-mode chromatographic materials for DNA isolation, including, e.g., the purification of plasmid DNA from crude cell lysates and for the preparation of DNA fragments before or after a polymerase chain reaction (PCR).</p>},
  author       = {Matos, Tiago and Bülow, Leif},
  issn         = {1875-5550},
  keyword      = {anion exchange,chromatography,DNA,elution,Hydrophobic interactions,multimodal,plasmid.},
  language     = {eng},
  number       = {1},
  pages        = {49--55},
  publisher    = {Bentham Science Publishers},
  series       = {Current protein & peptide science},
  title        = {Separation of Nucleic Acids Using Single- and Multimodal Chromatography},
  url          = {http://dx.doi.org/10.2174/1389203718666171024112556},
  volume       = {20},
  year         = {2019},
}