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Design of recombinant antibody microarrays for cell surface membrane proteomics

Dexlin Mellby, Linda LU ; Ingvarsson, Johan LU ; Frendeus, Björn; Borrebaeck, Carl LU and Wingren, Christer LU (2008) In Journal of Proteome Research 7(1). p.319-327
Abstract
Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be... (More)
Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Proteome Research
volume
7
issue
1
pages
319 - 327
publisher
The American Chemical Society
external identifiers
  • pmid:18047267
  • wos:000252154200038
  • scopus:38649117889
ISSN
1535-3893
DOI
10.1021/pr070257x
project
CREATE Health
language
English
LU publication?
yes
id
35e58c77-1d84-4fc7-ad97-d0058d92ce8d (old id 809740)
date added to LUP
2008-01-07 11:54:31
date last changed
2017-08-27 03:51:47
@article{35e58c77-1d84-4fc7-ad97-d0058d92ce8d,
  abstract     = {Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.},
  author       = {Dexlin Mellby, Linda and Ingvarsson, Johan and Frendeus, Björn and Borrebaeck, Carl and Wingren, Christer},
  issn         = {1535-3893},
  language     = {eng},
  number       = {1},
  pages        = {319--327},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {Design of recombinant antibody microarrays for cell surface membrane proteomics},
  url          = {http://dx.doi.org/10.1021/pr070257x},
  volume       = {7},
  year         = {2008},
}