Design of recombinant antibody microarrays for cell surface membrane proteomics
(2008) In Journal of Proteome Research 7(1). p.319-327- Abstract
- Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be... (More)
- Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/809740
- author
- Dexlin Mellby, Linda LU ; Ingvarsson, Johan LU ; Frendeus, Björn ; Borrebaeck, Carl LU and Wingren, Christer LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Proteome Research
- volume
- 7
- issue
- 1
- pages
- 319 - 327
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- pmid:18047267
- wos:000252154200038
- scopus:38649117889
- pmid:18047267
- ISSN
- 1535-3893
- DOI
- 10.1021/pr070257x
- language
- English
- LU publication?
- yes
- id
- 35e58c77-1d84-4fc7-ad97-d0058d92ce8d (old id 809740)
- date added to LUP
- 2016-04-01 11:44:01
- date last changed
- 2022-01-26 17:23:19
@article{35e58c77-1d84-4fc7-ad97-d0058d92ce8d, abstract = {{Generating proteomic maps of membrane proteins, common targets for therapeutic interventions and disease diagnostics, has turned out to be a major challenge. Antibody-based microarrays are among the novel rapidly evolving proteomic technologies that may enable global proteome analysis to be performed. Here, we have designed the first generation of a scaleable human recombinant scFv antibody microarray technology platform for cell surface membrane proteomics as well as glycomics targeting intact cells. The results showed that rapid and multiplexed profiling of the cell surface proteome (and glycome) could be performed in a highly specific and sensitive manner and that differential expression patterns due to external stimuli could be monitored.}}, author = {{Dexlin Mellby, Linda and Ingvarsson, Johan and Frendeus, Björn and Borrebaeck, Carl and Wingren, Christer}}, issn = {{1535-3893}}, language = {{eng}}, number = {{1}}, pages = {{319--327}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Journal of Proteome Research}}, title = {{Design of recombinant antibody microarrays for cell surface membrane proteomics}}, url = {{http://dx.doi.org/10.1021/pr070257x}}, doi = {{10.1021/pr070257x}}, volume = {{7}}, year = {{2008}}, }