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antibody microarray analysis of directly labelled proteomes

Wingren, Christer LU and Borrebaeck, Carl LU (2008) In Current Opinion in Biotechnology 19(1). p.55-61
Abstract
In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic... (More)
In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic standards for performing the antibody microarray data analysis have emerged, though general standard operating procedure(s) remains to be implemented. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Current Opinion in Biotechnology
volume
19
issue
1
pages
55 - 61
publisher
Elsevier
external identifiers
  • wos:000253767600010
  • scopus:39149088290
ISSN
0958-1669
DOI
10.1016/j.copbio.2007.11.010
project
CREATE Health
language
English
LU publication?
yes
id
4fb148f2-e9d0-4d8b-b1a6-de23c003f221 (old id 809746)
date added to LUP
2008-01-07 11:57:38
date last changed
2017-08-20 03:54:13
@article{4fb148f2-e9d0-4d8b-b1a6-de23c003f221,
  abstract     = {In recent years, the antibody microarray technology has made significant progress, going from proof-of-concept designs to established high-performing technology platforms capable of targeting non-fractionated complex proteomes. In these cross-disciplinary efforts, a particular focus has lately been placed on two key technological issues: the sample and data handling. To this end, robust protocols have been designed for direct labelling of whole proteomes compatible with a sensitive fluorescent-based sensing. Tagging of the proteins with biotin in a single-colour approach has, in many cases, proven to be the preferred approach. Furthermore, based on modified approaches, adopted from the DNA microarray field, the first bioinformatic standards for performing the antibody microarray data analysis have emerged, though general standard operating procedure(s) remains to be implemented.},
  author       = {Wingren, Christer and Borrebaeck, Carl},
  issn         = {0958-1669},
  language     = {eng},
  number       = {1},
  pages        = {55--61},
  publisher    = {Elsevier},
  series       = {Current Opinion in Biotechnology},
  title        = {antibody microarray analysis of directly labelled proteomes},
  url          = {http://dx.doi.org/10.1016/j.copbio.2007.11.010},
  volume       = {19},
  year         = {2008},
}