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Combinations of maggot excretions/secretions and antibiotics are effective against Staphylococcus aureus biofilms and the bacteria derived therefrom

van der Plas, Mariena J.A. LU ; Dambrot, Cheryl; Dogterom-Ballering, Heleen C.M.; Kruithof, Simone; van Dissel, Jaap T. and Nibbering, Peter H. (2010) In Journal of Antimicrobial Chemotherapy 65(5). p.917-923
Abstract

Objectives: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. Previously we reported that maggot excretions/secretions (ES) break down Staphylococcus aureus biofilms but do not kill the bacteria. As many antibiotics are not effective against biofilms we assessed the effect of combinations of ES and antibiotics on S. aureus biofilms and on the survival of the bacteria released from the biofilms. Methods: Effects of ES, antibiotics (vancomycin, daptomycin or clindamycin) and combinations thereof on S. aureus ATCC 29213 biofilms and bacterial viability were determined using microtitre plates and in vitro killing assays. Results: Vancomycin and daptomycin dose-dependently enhanced biofilm formation,... (More)

Objectives: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. Previously we reported that maggot excretions/secretions (ES) break down Staphylococcus aureus biofilms but do not kill the bacteria. As many antibiotics are not effective against biofilms we assessed the effect of combinations of ES and antibiotics on S. aureus biofilms and on the survival of the bacteria released from the biofilms. Methods: Effects of ES, antibiotics (vancomycin, daptomycin or clindamycin) and combinations thereof on S. aureus ATCC 29213 biofilms and bacterial viability were determined using microtitre plates and in vitro killing assays. Results: Vancomycin and daptomycin dose-dependently enhanced biofilm formation, whereas clindamycin reduced S. aureus biofilm size. Adding ES to antibiotic incubations caused a complete biofilm breakdown. After a lag time the bacteria derived from biofilms became susceptible to vancomycin and clindamycin, provided that the medium was refreshed. Daptomycin dose-dependently eliminated the biofilm-derived bacteria immediately. Furthermore, it was significantly more effective against bacteria derived from ES-exposed biofilms than those from control biofilms. ES did not affect the activity of the antibiotics against log-phase S. aureus. Conclusions: Combinations of maggot ES and antibiotics eliminate S. aureus biofilms and the bacteria derived therefrom.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Bacterial killing, Clindamycin, Daptomycin, Lucilia sericata, Vancomycin
in
Journal of Antimicrobial Chemotherapy
volume
65
issue
5
pages
7 pages
publisher
Oxford University Press
external identifiers
  • scopus:77953702881
ISSN
0305-7453
DOI
10.1093/jac/dkq042
language
English
LU publication?
no
id
80992c7f-5b72-411e-986c-d2ab852f1dec
date added to LUP
2018-01-15 10:55:05
date last changed
2018-09-30 04:48:28
@article{80992c7f-5b72-411e-986c-d2ab852f1dec,
  abstract     = {<p>Objectives: Maggots of the blowfly Lucilia sericata are used for the treatment of chronic wounds. Previously we reported that maggot excretions/secretions (ES) break down Staphylococcus aureus biofilms but do not kill the bacteria. As many antibiotics are not effective against biofilms we assessed the effect of combinations of ES and antibiotics on S. aureus biofilms and on the survival of the bacteria released from the biofilms. Methods: Effects of ES, antibiotics (vancomycin, daptomycin or clindamycin) and combinations thereof on S. aureus ATCC 29213 biofilms and bacterial viability were determined using microtitre plates and in vitro killing assays. Results: Vancomycin and daptomycin dose-dependently enhanced biofilm formation, whereas clindamycin reduced S. aureus biofilm size. Adding ES to antibiotic incubations caused a complete biofilm breakdown. After a lag time the bacteria derived from biofilms became susceptible to vancomycin and clindamycin, provided that the medium was refreshed. Daptomycin dose-dependently eliminated the biofilm-derived bacteria immediately. Furthermore, it was significantly more effective against bacteria derived from ES-exposed biofilms than those from control biofilms. ES did not affect the activity of the antibiotics against log-phase S. aureus. Conclusions: Combinations of maggot ES and antibiotics eliminate S. aureus biofilms and the bacteria derived therefrom.</p>},
  articleno    = {dkq042},
  author       = {van der Plas, Mariena J.A. and Dambrot, Cheryl and Dogterom-Ballering, Heleen C.M. and Kruithof, Simone and van Dissel, Jaap T. and Nibbering, Peter H.},
  issn         = {0305-7453},
  keyword      = {Bacterial killing,Clindamycin,Daptomycin,Lucilia sericata,Vancomycin},
  language     = {eng},
  month        = {02},
  number       = {5},
  pages        = {917--923},
  publisher    = {Oxford University Press},
  series       = {Journal of Antimicrobial Chemotherapy},
  title        = {Combinations of maggot excretions/secretions and antibiotics are effective against Staphylococcus aureus biofilms and the bacteria derived therefrom},
  url          = {http://dx.doi.org/10.1093/jac/dkq042},
  volume       = {65},
  year         = {2010},
}