Model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere.
(1986) In Applied and Environmental Microbiology 52(1). p.191-196- Abstract
An autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. Standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. The pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. The construction allowed a choice of membranes, which could be modified. The system was tested with a bacterium, isolated from rape plant roots (Brassica napus L.), that was inoculated on a hydrophilic membrane filter and allowed to develop into a biofilm. A defined medium with a... (More)
An autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. Standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. The pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. The construction allowed a choice of membranes, which could be modified. The system was tested with a bacterium, isolated from rape plant roots (Brassica napus L.), that was inoculated on a hydrophilic membrane filter and allowed to develop into a biofilm. A defined medium with a composition resembling that of natural rape root exudate was pumped through the membrane at 0.5 ml/h. Scanning electron microscopic examinations indicated that the inoculum formed microcolonies embedded in exopolymers evenly distributed over the membrane surface. The lipid composition and content of poly-beta-hydroxybutyrate in free-living and adhered cells were determined by gas chromatography. The bacterial consumption of amino acids in the exudate was also studied.
(Less)
- author
- Odham, G. ; Tunlid, A. LU ; Valeur, A. ; Sundin, P. and White, D. C.
- organization
- publishing date
- 1986-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied and Environmental Microbiology
- volume
- 52
- issue
- 1
- pages
- 191 - 196
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:0022757785
- pmid:11536565
- ISSN
- 0099-2240
- language
- English
- LU publication?
- yes
- id
- 809abe2b-c95f-4805-9ba1-ab092e2cfcdf
- date added to LUP
- 2019-10-23 17:32:08
- date last changed
- 2024-01-01 22:38:41
@article{809abe2b-c95f-4805-9ba1-ab092e2cfcdf,
abstract = {{<p>An autoclavable all-glass system for studying microbial dynamics at permeable surfaces is described. Standard hydrophobic or hydrophilic membranes (46-mm diameter) of various pore sizes were supported on a glass frit through which nutrient solutions were pumped by a peristaltic pump. The pump provided a precisely controlled flow at speeds of 0.5 to 500 ml of defined or natural cell exudates per h, which passed through the membrane into a receiving vessel. The construction allowed a choice of membranes, which could be modified. The system was tested with a bacterium, isolated from rape plant roots (Brassica napus L.), that was inoculated on a hydrophilic membrane filter and allowed to develop into a biofilm. A defined medium with a composition resembling that of natural rape root exudate was pumped through the membrane at 0.5 ml/h. Scanning electron microscopic examinations indicated that the inoculum formed microcolonies embedded in exopolymers evenly distributed over the membrane surface. The lipid composition and content of poly-beta-hydroxybutyrate in free-living and adhered cells were determined by gas chromatography. The bacterial consumption of amino acids in the exudate was also studied.</p>}},
author = {{Odham, G. and Tunlid, A. and Valeur, A. and Sundin, P. and White, D. C.}},
issn = {{0099-2240}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{191--196}},
publisher = {{American Society for Microbiology}},
series = {{Applied and Environmental Microbiology}},
title = {{Model system for studies of microbial dynamics at exuding surfaces such as the rhizosphere.}},
volume = {{52}},
year = {{1986}},
}