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Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi

Prachayasittikul, V; Lawung, R LU and Bulow, L LU (2000) In The Southeast Asian journal of tropical medicine and public health 31(1). p.4-80
Abstract

Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred... (More)

Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred resistance to various beta-lactam antibiotics including penicillin G, carbenicillin, piperacillin, cefazolin, cefoperazone, ampicillin-sulbactam, and amoxicillin-clavulanate but not to cefoxitin. Co-resistance to streptomycin, chloramphenicol and tetracycline was not detected. Beta-lactamase gene was located on the major pCb fragment of EcoRI and AatII cutting.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Cloning, Molecular, Drug Resistance, Microbial, Drug Resistance, Multiple, Escherichia coli, Haemophilus ducreyi, Plasmids, Transformation, Bacterial, beta-Lactam Resistance, beta-Lactamases
in
The Southeast Asian journal of tropical medicine and public health
volume
31
issue
1
pages
5 pages
publisher
Southeast Asian Ministers of Education Organisation
external identifiers
  • scopus:0034157052
ISSN
0125-1562
language
English
LU publication?
yes
id
80e7d688-22d6-4306-853d-a5530cfac6f1
alternative location
http://www.tm.mahidol.ac.th/seameo/2000_31_1/16-2397.pdf
date added to LUP
2016-04-18 15:58:27
date last changed
2017-05-02 18:09:49
@article{80e7d688-22d6-4306-853d-a5530cfac6f1,
  abstract     = {<p>Chancroid caused by Haemophilus ducreyi has been described as a significantly predisposing factor of HIV heterosexual transmission in an endemic region of both diseases. The fastidious, H. ducreyi has been reported world wide with various antimicrobial susceptibility patterns. A high tendency of drug resistances has generally been found among isolates derived in Thailand. In this study, the plasmids of H. ducreyi were isolated and analysed from 63 clinically derived organisms. Twenty-nine out of 63 isolates (46%) revealed the same plasmid profiles. Plasmid DNA was further cloned into Escherichia coli and transformants were selected. A 3.6 kb plasmid (pCb) carrying ampicillin resistance was subsequently identified. The pCb conferred resistance to various beta-lactam antibiotics including penicillin G, carbenicillin, piperacillin, cefazolin, cefoperazone, ampicillin-sulbactam, and amoxicillin-clavulanate but not to cefoxitin. Co-resistance to streptomycin, chloramphenicol and tetracycline was not detected. Beta-lactamase gene was located on the major pCb fragment of EcoRI and AatII cutting.</p>},
  author       = {Prachayasittikul, V and Lawung, R and Bulow, L},
  issn         = {0125-1562},
  keyword      = {Cloning, Molecular,Drug Resistance, Microbial,Drug Resistance, Multiple,Escherichia coli,Haemophilus ducreyi,Plasmids,Transformation, Bacterial,beta-Lactam Resistance,beta-Lactamases},
  language     = {eng},
  number       = {1},
  pages        = {4--80},
  publisher    = {Southeast Asian Ministers of Education Organisation},
  series       = {The Southeast Asian journal of tropical medicine and public health},
  title        = {Episome profiles and mobilizable beta-lactamase plasmid in Haemophilus ducreyi},
  volume       = {31},
  year         = {2000},
}