Advanced

Unusual Dynamics of Concentration Fluctuations in Solutions of Weakly Attractive Globular Proteins.

Bucciarelli, Saskia LU ; Casal-Dujat, Lucia LU ; De Michele, Cristiano; Sciortino, Francesco; Dhont, Jan; Bergenholtz, Johan LU ; Farago, Bela; Schurtenberger, Peter LU and Stradner, Anna LU (2015) In The Journal of Physical Chemistry Letters 6(22). p.4470-4474
Abstract
The globular protein γB-crystallin exhibits a complex phase behavior, where liquid-liquid phase separation characterized by a critical volume fraction ϕc = 0.154 and a critical temperature Tc = 291.8 K coexists with dynamical arrest on all length scales at volume fractions around ϕ ≈ 0.3-0.35, and an arrest line that extends well into the unstable region below the spinodal. However, although the static properties such as the osmotic compressibility and the static correlation length are in quantitative agreement with predictions for binary liquid mixtures, this is not the case for the dynamics of concentration fluctuations described by the dynamic structure factor S(q,t). Using a combination of dynamic light scattering and neutron spin echo... (More)
The globular protein γB-crystallin exhibits a complex phase behavior, where liquid-liquid phase separation characterized by a critical volume fraction ϕc = 0.154 and a critical temperature Tc = 291.8 K coexists with dynamical arrest on all length scales at volume fractions around ϕ ≈ 0.3-0.35, and an arrest line that extends well into the unstable region below the spinodal. However, although the static properties such as the osmotic compressibility and the static correlation length are in quantitative agreement with predictions for binary liquid mixtures, this is not the case for the dynamics of concentration fluctuations described by the dynamic structure factor S(q,t). Using a combination of dynamic light scattering and neutron spin echo measurements, we demonstrate that the competition between critical slowing down and dynamical arrest results in a much more complex wave vector dependence of S(q,t) than previously anticipated. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
The Journal of Physical Chemistry Letters
volume
6
issue
22
pages
4470 - 4474
publisher
The American Chemical Society
external identifiers
  • pmid:26505877
  • wos:000365460700004
  • scopus:84947969262
ISSN
1948-7185
DOI
10.1021/acs.jpclett.5b02092
language
English
LU publication?
yes
id
f48b2f53-49f2-4bd9-ac71-13cafe50f2b8 (old id 8148219)
date added to LUP
2015-11-18 16:59:59
date last changed
2017-07-02 03:55:53
@article{f48b2f53-49f2-4bd9-ac71-13cafe50f2b8,
  abstract     = {The globular protein γB-crystallin exhibits a complex phase behavior, where liquid-liquid phase separation characterized by a critical volume fraction ϕc = 0.154 and a critical temperature Tc = 291.8 K coexists with dynamical arrest on all length scales at volume fractions around ϕ ≈ 0.3-0.35, and an arrest line that extends well into the unstable region below the spinodal. However, although the static properties such as the osmotic compressibility and the static correlation length are in quantitative agreement with predictions for binary liquid mixtures, this is not the case for the dynamics of concentration fluctuations described by the dynamic structure factor S(q,t). Using a combination of dynamic light scattering and neutron spin echo measurements, we demonstrate that the competition between critical slowing down and dynamical arrest results in a much more complex wave vector dependence of S(q,t) than previously anticipated.},
  author       = {Bucciarelli, Saskia and Casal-Dujat, Lucia and De Michele, Cristiano and Sciortino, Francesco and Dhont, Jan and Bergenholtz, Johan and Farago, Bela and Schurtenberger, Peter and Stradner, Anna},
  issn         = {1948-7185},
  language     = {eng},
  number       = {22},
  pages        = {4470--4474},
  publisher    = {The American Chemical Society},
  series       = {The Journal of Physical Chemistry Letters},
  title        = {Unusual Dynamics of Concentration Fluctuations in Solutions of Weakly Attractive Globular Proteins.},
  url          = {http://dx.doi.org/10.1021/acs.jpclett.5b02092},
  volume       = {6},
  year         = {2015},
}